Home LiteratureArticle Details
PMID: 6253949 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of a 5S ribosomal RNA gene in the sea urchin Lytechinus variegatus.

Nucleic acids research ·Vol. 8 ·No. 8 ·1980-04-25 ·Pages 1839-53

Lu AL, Steege DA, Stafford DW

Abstract

The nucleotide sequence of a cloned DNA fragment corresponding to a gene for 5S ribosomal RNA from the sea urchin Lytechinus variegatus has been determined. This sequence is representative of the dominant species of 5S rRNA labelled in vivo with 32pO4 during the cleavage stage of Lytechninus embryonic development.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Molecular Weight Oligodeoxyribonucleotides/analysis Plasmids RNA, Ribosomal/biosynthesis Sea Urchins
Chemicals
DNA, Recombinant Oligodeoxyribonucleotides RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lu A L
Steege D A
Stafford D W
References (38)
38 references, click to expand
  1. Purification and some characteristics of 5S DNA from Xenopus laevis.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3175-9 PMID: 5289255
  2. Minor components in transfer RNA: their characterization, location, and function.
    Prog Nucleic Acid Res Mol Biol. 1972;12:49-85 PMID: 4557059
  3. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  4. Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis.
    Nat New Biol. 1973 Jan 3;241(105):7-12 PMID: 4512331
  5. General method for the isolation of plasmid deoxyribonucleic acid.
    J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
  6. Physical linkage of the 5 S cistrons to the 18 S and 28 S ribosomal RNA cistrons in Saccharomyces cerevisiae.
    J Mol Biol. 1973 Sep 25;79(3):521-30 PMID: 4585976
  7. Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes.
    J Mol Biol. 1973 Dec 15;81(3):419-23 PMID: 4588280
  8. The structure and evolution of ribosomal and 5S DNAs in Xenopus laevis and Xenopus mulleri.
    Cold Spring Harb Symp Quant Biol. 1974;38:501-5 PMID: 4524770
  9. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.
    Biochemistry. 1974 Aug 13;13(17):3606-15 PMID: 4858491
  10. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  11. Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2525-9 PMID: 1058472
  12. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  13. Genetic analysis of the 5S RNA genes in Drosophila melanogaster.
    Genetics. 1975 Nov;81(3):515-23 PMID: 812776
  14. Sequences of 5S ribosomal RNA from Xenopus mulleri and the evolution of 5S gene-coding sequences.
    Cell. 1976 Aug;8(4):485-93 PMID: 986255
  15. EcoRI endonuclease. Physical and catalytic properties of the homogenous enzyme.
    J Biol Chem. 1976 Oct 10;251(19):5866-74 PMID: 786985
  16. A general method for isolation of high molecular weight DNA from eukaryotes.
    Nucleic Acids Res. 1976 Sep;3(9):2303-8 PMID: 987581
  17. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit.
    Cell. 1976 Nov;9(3):431-8 PMID: 1033038
  18. Organization of the ribosomal RNA genes of Dictyostelium discoideum: mapping of the nontranscribed spacer regions.
    Cell. 1976 Dec;9(4 Pt 1):605-13 PMID: 1034500
  19. Utilization of the guanylyltransferase and methyltransferases of vaccinia virus to modify and identify the 5'-terminals of heterologous RNA species.
    Biochem Biophys Res Commun. 1977 Jan 24;74(2):374-83 PMID: 836293
  20. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  21. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  22. Nucleotide sequence of the yeast 5S ribosomal RNA gene and adjacent putative control regions.
    Nature. 1977 Jun 16;267(5612):641-3 PMID: 327335
  23. Promotor region for yeast 5S ribosomal RNA.
    Nature. 1977 Jun 16;267(5612):643-5 PMID: 327336
  24. A simple, general procedure for purifying restriction endonucleases.
    Nucleic Acids Res. 1977 Aug;4(8):2561-72 PMID: 909783
  25. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.
    J Biol Chem. 1977 Nov 25;252(22):8118-25 PMID: 334774
  26. Studies of low molecular weight RNA from cells infected with adenovirus 2. I. The sequences at the 3' end of VA-RNA I.
    J Biol Chem. 1977 Dec 25;252(24):9032-42 PMID: 318160
  27. Computer analysis of nucleic acid regulatory sequences.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4401-5 PMID: 270683
  28. The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region.
    Cell. 1978 Apr;13(4):717-25 PMID: 566164
  29. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  30. Adenovirus DNA-directed transcription of 5.5S RNA in vitro.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2175-9 PMID: 276858
  31. A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
    Cell. 1978 Nov;15(3):1077-86 PMID: 569551
  32. Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.
    Cell. 1978 Dec;15(4):1145-56 PMID: 264240
  33. Collection of published 5S and 5.8S RNA sequences and their precursors.
    Nucleic Acids Res. 1979 Jan;6(1):r29-r44 PMID: 424283
  34. Transcription of cloned Xenopus 5S RNA genes by X. laevis RNA polymerase III in reconstituted systems.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):136-40 PMID: 284325
  35. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):381-5 PMID: 284354
  36. Metabolism of low molecular weight ribonucleic acids in early sea urchin embryos.
    Biochemistry. 1979 Apr 3;18(7):1353-60 PMID: 427117
  37. Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
    J Biol Chem. 1979 Jul 10;254(13):6163-73 PMID: 447704
  38. Direct chemical method for sequencing RNA.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760-4 PMID: 377283
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1980-04-25
Pages
1839-53
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324039
Subset
IM
Grants
NCI NIH HHS · 1P01-CA-14236-05 · United States
NIAID NIH HHS · AI 14882 · United States
NIGMS NIH HHS · GM 24233 · United States
Databases
GENBANK
M10468
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]