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PMID: 11788771 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants.

Plant physiology ·Vol. 128 ·No. 1 ·2002-01-00 ·Pages 256-70

Chico JM, Raíces M, Téllez-Iñón MT, Ulloa RM

Abstract

A full-length cDNA clone (LeCDPK1) from tomato (Lycopersicon esculentum) encoding a calcium-dependent protein kinase (CDPK) was isolated by screening a cDNA library from tomato cell cultures exposed to Cladosporium fulvum elicitor preparations. The predicted amino acid sequence of the cDNA reveals a high degree of similarity with other members of the CDPK family. LeCDPK1 has a putative N-terminal myristoylation sequence and presents a possible palmitoylation site. The in vitro translated protein conserves the biochemical properties of a member of the CDPK family. In addition, CDPK activity was detected in soluble and particulate extracts of tomato leaves. Basal levels of LeCDPK1 mRNA were detected by northern-blot analysis in roots, stems, leaves, and flowers of tomato plants. The expression of LeCDPK1 was rapidly and transiently enhanced in detached tomato leaves treated with pathogen elicitors and H2O2. Moreover, when tomato greenhouse plants were subjected to mechanical wounding, a transient increase of LeCDPK1 steady-state mRNA levels was detected locally at the site of the injury and systemically in distant non-wounded leaves. The increase observed in LeCDPK1 mRNA upon wounding correlates with an increase in the amount and in the activity of a soluble CDPK detected in extracts of tomato leaves, suggesting that this kinase is part of physiological plant defense mechanisms against biotic or abiotic attacks.

MeSH Terms
Adaptation, Physiological Amino Acid Sequence Blotting, Northern Blotting, Southern Blotting, Western Calcium-Binding Proteins/genetics,isolation & purification,metabolism Cloning, Molecular Colletotrichum/growth & development Gene Expression Regulation, Enzymologic Immunity, Innate/genetics Lycopersicon esculentum/enzymology,genetics Molecular Sequence Data Plant Diseases/microbiology Plant Leaves/enzymology,genetics Protein Kinases/genetics,isolation & purification,metabolism RNA, Messenger/genetics,metabolism Sequence Homology, Amino Acid Signal Transduction
Chemicals
Calcium-Binding Proteins RNA, Messenger Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chico José Manuel
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Vuelta de Obligado 2490 2(do) piso, 1428 Buenos Aires, Argentina.
Raíces Marcela
Téllez-Iñón María Teresa
Ulloa Rita María
References (54)
54 references, click to expand
  1. Oligosaccharide signalling in plants.
    Annu Rev Cell Biol. 1987;3:295-317 PMID: 3318878
  2. Expression of the Arabidopsis abi1-1 mutant allele inhibits proteinase inhibitor wound-induction in tomato.
    Plant J. 1998 Sep;15(6):765-71 PMID: 9807815
  3. Octadecanoid Precursors of Jasmonic Acid Activate the Synthesis of Wound-Inducible Proteinase Inhibitors.
    Plant Cell. 1992 Feb;4(2):129-134 PMID: 12297644
  4. Identification of Ser-543 as the major regulatory phosphorylation site in spinach leaf nitrate reductase.
    Plant Cell. 1996 Mar;8(3):505-17 PMID: 8721752
  5. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  6. Involvement of the octadecanoid pathway and protein phosphorylation in fungal elicitor-induced expression of terpenoid indole alkaloid biosynthetic genes in catharanthus roseus
    Plant Physiol. 1999 Apr;119(4):1289-96 PMID: 10198087
  7. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  8. Rapid Avr9- and Cf-9 -dependent activation of MAP kinases in tobacco cell cultures and leaves: convergence of resistance gene, elicitor, wound, and salicylate responses.
    Plant Cell. 1999 Feb;11(2):273-87 PMID: 9927644
  9. Transcriptional control of plant genes responsive to pathogens.
    Curr Opin Plant Biol. 1998 Aug;1(4):311-5 PMID: 10066598
  10. Rapid isolation of high molecular weight plant DNA.
    Nucleic Acids Res. 1980 Oct 10;8(19):4321-5 PMID: 7433111
  11. Molecular characterization of StCDPK1, a calcium-dependent protein kinase from Solanum tuberosum that is induced at the onset of tuber development.
    Plant Mol Biol. 2001 Jul;46(5):591-601 PMID: 11516152
  12. Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity.
    Cell. 1998 Mar 20;92(6):773-84 PMID: 9529253
  13. Cloning, expression and N-terminal myristoylation of CpCPK1, a calcium-dependent protein kinase from zucchini (Cucurbita pepo L.).
    Plant Mol Biol. 1999 Jan;39(2):199-208 PMID: 10080688
  14. Characterization of an Arabidopsis lipoxygenase gene responsive to methyl jasmonate and wounding.
    Plant Physiol. 1993 Dec;103(4):1133-7 PMID: 8290626
  15. Changes in Calcium-Dependent Protein Kinase Activity during in Vitro Tuberization in Potato.
    Plant Physiol. 1996 Dec;112(4):1541-1550 PMID: 12226463
  16. Molecular genetics of plant disease resistance.
    Science. 1995 May 5;268(5211):661-7 PMID: 7732374
  17. Detection of several mRNA species in rice phloem sap.
    Plant Cell Physiol. 1998 Aug;39(8):895-7 PMID: 9787465
  18. Modulation of plasma membrane H+-ATPase activity differentially activates wound and pathogen defense responses in tomato plants.
    Plant Cell. 1999 Feb;11(2):263-72 PMID: 9927643
  19. Signal transduction in the wound response of tomato plants.
    Philos Trans R Soc Lond B Biol Sci. 1998 Sep 29;353(1374):1495-510 PMID: 9800210
  20. A gene encoding a chloroplast-targeted lipoxygenase in tomato leaves is transiently induced by wounding, systemin, and methyl jasmonate.
    Plant Physiol. 1997 Jul;114(3):1085-93 PMID: 9232884
  21. Hydrogen peroxide-induced gene expression in Arabidopsis thaliana.
    Free Radic Biol Med. 2000 Mar 1;28(5):773-8 PMID: 10754273
  22. Resistance gene-dependent activation of a calcium-dependent protein kinase in the plant defense response.
    Plant Cell. 2000 May;12(5):803-16 PMID: 10810151
  23. General roles of abscisic and jasmonic acids in gene activation as a result of mechanical wounding.
    Plant Cell. 1992 Sep;4(9):1157-70 PMID: 1392612
  24. H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response.
    Cell. 1994 Nov 18;79(4):583-93 PMID: 7954825
  25. Receptor-mediated activation of a MAP kinase in pathogen defense of plants.
    Science. 1997 Jun 27;276(5321):2054-7 PMID: 9197271
  26. Map-based cloning of a protein kinase gene conferring disease resistance in tomato.
    Science. 1993 Nov 26;262(5138):1432-6 PMID: 7902614
  27. Resistance response physiology and signal transduction.
    Curr Opin Plant Biol. 1998 Aug;1(4):305-10 PMID: 10066609
  28. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants.
    Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2940-5 PMID: 10717008
  29. Wound-Induced Proteinase Inhibitor in Plant Leaves: A Possible Defense Mechanism against Insects.
    Science. 1972 Feb 18;175(4023):776-7 PMID: 17836138
  30. Plant paralog to viral movement protein that potentiates transport of mRNA into the phloem.
    Science. 1999 Jan 1;283(5398):94-8 PMID: 9872750
  31. Characterization of eight new members of the calmodulin-like domain protein kinase gene family from Arabidopsis thaliana.
    Plant Mol Biol. 1996 May;31(2):405-12 PMID: 8756605
  32. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  33. Defense-related proteins in higher plants.
    Annu Rev Biochem. 1990;59:873-907 PMID: 2197993
  34. A polypeptide from tomato leaves induces wound-inducible proteinase inhibitor proteins.
    Science. 1991 Aug 23;253(5022):895-7 PMID: 17751827
  35. Reversible protein phosphorylation regulates jasmonic acid-dependent and -independent wound signal transduction pathways in Arabidopsis thaliana.
    Plant J. 1998 Jan;13(2):153-65 PMID: 9680973
  36. Wound signalling in plants.
    J Exp Bot. 2001 Jan;52(354):1-9 PMID: 11181708
  37. Signal recognition and transduction involved in plant disease resistance.
    Essays Biochem. 1997;32:87-99 PMID: 9493013
  38. Reactive oxygen species and the regulation of cell death by the Bcl-2 gene family.
    Biochim Biophys Acta. 1995 May 24;1271(1):63-6 PMID: 7599227
  39. A stress-induced calcium-dependent protein kinase from Mesembryanthemum crystallinum phosphorylates a two-component pseudo-response regulator.
    Plant J. 2000 Dec;24(5):679-91 PMID: 11123806
  40. Phloem long-distance transport of CmNACP mRNA: implications for supracellular regulation in plants.
    Development. 1999 Oct;126(20):4405-19 PMID: 10498677
  41. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21.
    Science. 1995 Dec 15;270(5243):1804-6 PMID: 8525370
  42. Signal perception and transduction in plant defense responses.
    Genes Dev. 1997 Jul 1;11(13):1621-39 PMID: 9224713
  43. Receptor-mediated increase in cytoplasmic free calcium required for activation of pathogen defense in parsley.
    Plant Cell. 2000 Aug;12(8):1425-40 PMID: 10948260
  44. Induction of 12-oxo-phytodienoic acid in wounded plants and elicited plant cell cultures.
    Plant Physiol. 1997 Nov;115(3):1057-64 PMID: 9390438
  45. The differential expression of wound-inducible lipoxygenase genes in soybean leaves.
    Plant Physiol. 1996 Jan;110(1):287-99 PMID: 8587989
  46. Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding, systemin, and methyl jasmonate.
    Plant Cell. 2001 Jan;13(1):179-91 PMID: 11158538
  47. Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves.
    Proc Natl Acad Sci U S A. 1990 Oct;87(19):7713-6 PMID: 11607107
  48. Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway.
    Proc Natl Acad Sci U S A. 1999 May 25;96(11):6553-7 PMID: 10339626
  49. A wound- and systemin-inducible polygalacturonase in tomato leaves.
    Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1756-60 PMID: 9990097
  50. Activation of the tobacco SIP kinase by both a cell wall-derived carbohydrate elicitor and purified proteinaceous elicitins from Phytophthora spp.
    Plant Cell. 1998 Mar;10(3):435-50 PMID: 9501116
  51. Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9851-5 PMID: 16594093
  52. The octadecanoid signalling pathway in plants mediates a response to ultraviolet radiation.
    Nature. 1996 Oct 31;383(6603):826-9 PMID: 8893008
  53. Membrane localization of a rice calcium-dependent protein kinase (CDPK) is mediated by myristoylation and palmitoylation.
    Plant J. 2000 Nov;24(4):429-35 PMID: 11115124
  54. Characterization and localization of a wound-inducible type I serine-carboxypeptidase from leaves of tomato plants (Lycopersicon esculentum Mill.).
    Planta. 2001 Jan;212(2):222-30 PMID: 11216843
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.128.1.256
Published
2002-01-00
Pages
256-70
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC148989
Subset
IM
Databases
GENBANK
AF363784
External Links
PubMed source DOI: 10.1104/pp.128.1.256 PMC Full Text View journal details
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