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PMID: 11854417 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Golgi vesicle proteins are linked to the assembly of an actin complex defined by mAbp1.

Molecular biology of the cell ·Vol. 13 ·No. 2 ·2002-02-00 ·Pages 621-31

Fucini RV, Chen JL, Sharma C, Kessels MM, Stamnes M

Abstract

Recent studies indicate that regulation of the actin cytoskeleton is important for protein trafficking, but its precise role is unclear. We have characterized the ARF1-dependent assembly of actin on the Golgi apparatus. Actin recruitment involves Cdc42/Rac and requires the activation of the Arp2/3 complex. Although the actin-binding proteins mAbp1 (SH3p7) and drebrin share sequence homology, they are differentially segregated into two distinct ARF-dependent actin complexes. The binding of Cdc42 and mAbp1, which localize to the Golgi apparatus, but not drebrin, is blocked by occupation of the p23 cargo-protein-binding site on coatomer. Exogenously expressed mAbp1 is mislocalized and inhibits Golgi transport in whole cells. The ability of ARF, vesicle-coat proteins, and cargo to direct the assembly of cytoskeletal structures helps explain how only a handful of vesicle types can mediate the numerous trafficking steps in the cell.

MeSH Terms
ADP-Ribosylation Factor 1/physiology Actins/physiology Animals Cattle Cytoskeleton/physiology Golgi Apparatus/physiology Macromolecular Substances Membrane Proteins/physiology Microfilament Proteins/physiology Rats Secretory Vesicles/physiology cdc42 GTP-Binding Protein/physiology rho GTP-Binding Proteins/physiology src Homology Domains
Chemicals
Actins Dbnl protein, rat Macromolecular Substances Membrane Proteins Microfilament Proteins ADP-Ribosylation Factor 1 cdc42 GTP-Binding Protein rho GTP-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fucini Raymond V
Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA.
Chen Ji-Long
Sharma Catherine
Kessels Michael M
Stamnes Mark
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.01-11-0547
Published
2002-02-00
Pages
621-31
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC65654
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.01-11-0547 PMC Full Text View journal details
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