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PMID: 11861847 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Physical association of the K3 protein of gamma-2 herpesvirus 68 with major histocompatibility complex class I molecules with impaired peptide and beta(2)-microglobulin assembly.

Journal of virology ·Vol. 76 ·No. 6 ·2002-03-00 ·Pages 2796-803

Yu YY, Harris MR, Lybarger L, Kimpler LA, Myers NB, Virgin HW, Hansen TH

Abstract

To persist in the presence of an active immune system, viruses encode proteins that decrease expression of major histocompatibility complex class I molecules by using a variety of mechanisms. For example, murine gamma-2 herpesvirus 68 expresses the K3 protein, which causes the rapid turnover of nascent class I molecules. In this report we show that certain mouse class I alleles are more susceptible than others to K3-mediated down regulation. Prior to their rapid degradation, class I molecules in K3-expressing cells exhibit impaired assembly with beta(2)-microglobulin. Furthermore, K3 is detected predominantly in association with class I molecules lacking assembly with high-affinity peptides, including class I molecules associated with the peptide loading complex TAP/tapasin/calreticulin. The detection of K3 with class I assembly intermediates raises the possibility that molecular chaperones involved in class I assembly are involved in K3-mediated class I regulation.

MeSH Terms
Animals Down-Regulation Gammaherpesvirinae/pathogenicity,physiology Herpesviridae Infections/virology Histocompatibility Antigens Class I/chemistry,genetics,physiology L Cells Mice Peptides/metabolism Protein Folding Viral Proteins/physiology beta 2-Microglobulin/metabolism
Chemicals
Histocompatibility Antigens Class I Peptides Viral Proteins beta 2-Microglobulin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Yu Y Y Lawrence
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Harris Michael R
Lybarger Lonnie
Kimpler Lisa A
Myers Nancy B
Virgin Herbert W
Hansen Ted H
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.76.6.2796-2803.2002
Published
2002-03-00
Pages
2796-803
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC135993
Subset
IM
Grants
NIAID NIH HHS · R01 AI042793 · United States
NHLBI NIH HHS · R01 HL060090 · United States
NIAID NIH HHS · AI01498 · United States
NIAID NIH HHS · AI16789 · United States
NIAID NIH HHS · AI46553 · United States
NIAID NIH HHS · AI45019 · United States
NCI NIH HHS · CA74730 · United States
NHLBI NIH HHS · HL60090 · United States
NIAID NIH HHS · T32 AI007163 · United States
NIAID NIH HHS · AI07163 · United States
NCI NIH HHS · R01 CA074730 · United States
NIAID NIH HHS · R01 AI046553 · United States
NIAID NIH HHS · R21 AI042793 · United States
NIAID NIH HHS · AI42793 · United States
NIAID NIH HHS · R01 AI045019 · United States
External Links
PubMed source DOI: 10.1128/jvi.76.6.2796-2803.2002 PMC Full Text View journal details
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