Home LiteratureArticle Details
PMID: 11879637 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Conserved organization of centromeric chromatin in flies and humans.

Developmental cell ·Vol. 2 ·No. 3 ·2002-03-00 ·Pages 319-30

Blower MD, Sullivan BA, Karpen GH

Abstract

Recent studies have highlighted the importance of centromere-specific histone H3-like (CENP-A) proteins in centromere function. We show that Drosophila CID and human CENP-A appear at metaphase as a three-dimensional structure that lacks histone H3. However, blocks of CID/CENP-A and H3 nucleosomes are linearly interspersed on extended chromatin fibers, and CID is close to H3 nucleosomes in polynucleosomal preparations. When CID is depleted by RNAi, it is replaced by H3, demonstrating flexibility of centromeric chromatin organization. Finally, contrary to models proposing that H3 and CID/CENP-A nucleosomes are replicated at different times in S phase, we show that interspersed H3 and CID/CENP-A chromatin are replicated concurrently during S phase in humans and flies. We propose that the unique structural arrangement of CID/CENP-A and H3 nucleosomes presents centromeric chromatin to the poleward face of the condensing mitotic chromosome.

MeSH Terms
Animals Autoantigens Centromere Protein A Chromatin/chemistry,physiology Chromosomal Proteins, Non-Histone/analysis Chromosomes/chemistry,physiology DNA-Binding Proteins Drosophila Drosophila Proteins Evolution, Molecular Histones/analysis,metabolism Humans Kinetochores/chemistry,physiology Metaphase/physiology Nucleosomes/chemistry Phosphorylation Protein Structure, Tertiary
Chemicals
Autoantigens CENPA protein, human Centromere Protein A Chromatin Chromosomal Proteins, Non-Histone Cid protein, Drosophila DNA-Binding Proteins Drosophila Proteins Histones Nucleosomes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blower Michael D
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Sullivan Beth A
Karpen Gary H
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Article Info
Journal
Developmental cell
Abbr.
Dev Cell
ISSN
1534-5807
DOI
10.1016/s1534-5807(02)00135-1
Published
2002-03-00
Pages
319-30
Language
English
Region
United States
NLM ID
101120028
PMCID
PMC3192492
Subset
IM
Grants
NIGMS NIH HHS · R01 GM054549-04 · United States
NIGMS NIH HHS · 5R01 GM54549 · United States
External Links
PubMed source DOI: 10.1016/s1534-5807(02)00135-1 PMC Full Text View journal details
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