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PMID: 11884628 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential expression and requirements for Schizosaccharomyces pombe RAD52 homologs in DNA repair and recombination.

Nucleic acids research ·Vol. 30 ·No. 6 ·2002-03-15 ·Pages 1316-24

van den Bosch M, Zonneveld JB, Vreeken K, de Vries FA, Lohman PH, Pastink A

Abstract

In fission yeast two RAD52 homologs have been identified, rad22A(+) and rad22B(+). Two-hybrid experiments and GST pull-down assays revealed physical interaction between Rad22A and Rad22B, which is dependent on the N-terminal regions. Interaction with Rhp51 is dependent on the C-terminal parts of either protein. Both Rad22A and Rad22B also interact with RPA. The expression of rad22B(+) in mitotically dividing cells is very low in comparison with rad22A(+) but is strongly enhanced after induction of meiosis, in contrast to rad22A(+). Rad22B mutant cells are not hypersensitive to DNA-damaging agents (X-rays, UV and cisplatin) and display normal levels of recombination. In these respects the Schizosaccharomyces pombe rad22B mutant resembles the weak phenotype of vertebrate cells deficient for RAD52. Mutation of rad22A(+) leads to severe sensitivity to DNA-damaging agents and to defects in recombination. In a rad22Arad22B double mutant a further increase in sensitivity to DNA-damaging agents and additional mitotic recombination defects were observed. The data presented here indicate that Rad22A and Rad22B have overlapping roles in repair and recombination, although specialized functions for each protein cannot be excluded.

MeSH Terms
DNA Helicases/metabolism DNA Repair DNA, Fungal/genetics DNA-Binding Proteins/genetics,metabolism,physiology Fungal Proteins/genetics,metabolism,physiology Meiosis Mutation Phenotype Rad51 Recombinase Rad52 DNA Repair and Recombination Protein Recombination, Genetic Schizosaccharomyces/genetics,metabolism Schizosaccharomyces pombe Proteins/genetics,metabolism,physiology Two-Hybrid System Techniques
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins RHP51 protein, S pombe Rad22B protein, S pombe Rad52 DNA Repair and Recombination Protein Schizosaccharomyces pombe Proteins rad52 protein, S pombe Rad51 Recombinase DNA Helicases Rhp54 protein, S pombe
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
van den Bosch Michael
MGC Department of Radiation Genetics and Chemical Mutagenesis, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands.
Zonneveld José B M
Vreeken Kees
de Vries Femke A T
Lohman Paul H M
Pastink Albert
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/30.6.1316
Published
2002-03-15
Pages
1316-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC101351
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/30.6.1316 PMC Full Text View journal details
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