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PMID: 11891305 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Hydrogen peroxide homeostasis: activation of plant catalase by calcium/calmodulin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 6 ·2002-03-19 ·Pages 4097-102

Yang T, Poovaiah BW

Abstract

Environmental stimuli such as UV, pathogen attack, and gravity can induce rapid changes in hydrogen peroxide (H(2)O(2)) levels, leading to a variety of physiological responses in plants. Catalase, which is involved in the degradation of H(2)O(2) into water and oxygen, is the major H(2)O(2)-scavenging enzyme in all aerobic organisms. A close interaction exists between intracellular H(2)O(2) and cytosolic calcium in response to biotic and abiotic stresses. Studies indicate that an increase in cytosolic calcium boosts the generation of H(2)O(2). Here we report that calmodulin (CaM), a ubiquitous calcium-binding protein, binds to and activates some plant catalases in the presence of calcium, but calcium/CaM does not have any effect on bacterial, fungal, bovine, or human catalase. These results document that calcium/CaM can down-regulate H(2)O(2) levels in plants by stimulating the catalytic activity of plant catalase. Furthermore, these results provide evidence indicating that calcium has dual functions in regulating H(2)O(2) homeostasis, which in turn influences redox signaling in response to environmental signals in plants.

Keywords
NASA Discipline Plant Biology Non-NASA Center
MeSH Terms
Amino Acid Sequence Arabidopsis/drug effects,enzymology,genetics Arabidopsis Proteins/chemistry,genetics,isolation & purification,metabolism Calcium/pharmacology Calcium-Calmodulin-Dependent Protein Kinases/metabolism Catalase/chemistry,genetics,isolation & purification,metabolism Catalysis Cloning, Molecular Enzyme Activation Homeostasis Hydrogen Peroxide/metabolism Models, Biological Molecular Sequence Data Peroxisomes/enzymology Plant Leaves/enzymology Protein Binding Sequence Alignment Tobacco/enzymology
Chemicals
Arabidopsis Proteins Hydrogen Peroxide Catalase Calcium-Calmodulin-Dependent Protein Kinases Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yang T
Laboratory of Plant Molecular Biology and Physiology, Department of Horticulture, Washington State University, Pullman, WA 99164-6414, USA.
Poovaiah B W
Investigators
1 investigators, click to expand
Poovaiah B W
WA St U, Pullman
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.052564899
Published
2002-03-19
Epub
2002-00-12
Pages
4097-102
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC122654
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.052564899 PMC Full Text View journal details
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