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PMID: 11892807 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Torsional stress induces left-handed helical stretches in DNA of natural base sequence: circular dichroism and antibody binding.

The EMBO journal ·Vol. 2 ·No. 9 ·1983-00-00 ·Pages 1531-5

Di Capua E, Stasiak A, Koller T, Brahms S, Thomae R, Pohl FM

Abstract

Above a threshold of torsional stress, the c.d. spectrum of covalently closed circular DNA of natural base sequence acquires a Z-like contribution and antibodies raised against Z-DNA are bound. Mapping of the antibody binding sites by electron microscopy reveals sites which correlate with stretches enriched in alternating purine-pyrimidine sequences and GC base pairs.

MeSH Terms
Animals Antibodies Base Sequence Binding Sites Circular Dichroism DNA, Circular/chemistry,genetics,immunology,ultrastructure Globins/genetics In Vitro Techniques Mice Microscopy, Electron Nucleic Acid Conformation Plasmids/chemistry,genetics,immunology,ultrastructure Rabbits Stress, Mechanical
Chemicals
Antibodies DNA, Circular Globins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Di Capua E
Institut für Zellbiologie, ETH, Hönggerberg, Zürich, Switzerland.
Stasiak A
Koller T
Brahms S
Thomae R
Pohl F M
References (21)
21 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1531-5
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555318
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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