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PMID: 7188247 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Visualization of Z sequences in form V of pBR322 by immuno-electron microscopy.

The EMBO journal ·Vol. 1 ·No. 10 ·1982-00-00 ·Pages 1149-53

Lang MC, Malfoy B, Freund AM, Daune M, Leng M

Abstract

Form V DNA has been prepared from pBR322 DNA by annealing covalently closed complementary single strands. Specific rabbit antibodies to Z-DNA were shown by radioimmunoassay and electron microscopy to react with form V DNA of pBR322. The bound antibodies were visualized either directly (on synthetic polynucleotides in Z-form), or after reaction with goat anti-rabbit immunoglobulin labeled with ferritin (on form V DNA).

MeSH Terms
Antigen-Antibody Complex Base Sequence DNA Immune Sera Microscopy, Electron Nucleic Acid Conformation Plasmids Polydeoxyribonucleotides Radioimmunoassay
Chemicals
Antigen-Antibody Complex Immune Sera Polydeoxyribonucleotides poly(dC-dG) DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lang M C
Malfoy B
Freund A M
Daune M
Leng M
References (24)
24 references, click to expand
  1. A new preparation method for dark-field electron microscopy of biomacromolecules.
    J Ultrastruct Res. 1971 Apr;35(1):147-67 PMID: 4931423
  2. Right-handed and left-handed DNA: studies of B- and Z-DNA by using proton nuclear Overhauser effect and P NMR.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1413-7 PMID: 6951185
  3. Polymorphism of a synthetic DNA in solution.
    Nature. 1976 Mar 25;260(5549):365-6 PMID: 1256581
  4. Ion effects on ligand-nucleic acid interactions.
    J Mol Biol. 1976 Oct 25;107(2):145-58 PMID: 1003464
  5. Preparation and characterization of form V DNA, the duplex DNA resulting from association of complementary, circular single-stranded DNA.
    J Mol Biol. 1979 Jun 15;131(1):21-40 PMID: 490644
  6. Molecular structure of a left-handed double helical DNA fragment at atomic resolution.
    Nature. 1979 Dec 13;282(5740):680-6 PMID: 514347
  7. Electron microscopic visualization of N-acetoxy-N-2-acetylaminofluorene binding sites in ColE1 DNA by means of specific antibodies.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6076-80 PMID: 293703
  8. Left-handed DNA helices.
    Nature. 1980 Feb 21;283(5749):743-5 PMID: 7354864
  9. High-salt d(CpGpCpG), a left-handed Z' DNA double helix.
    Nature. 1980 Aug 7;286(5773):567-73 PMID: 7402336
  10. Salt-dependent dynamic structure of poly(dG-dC) x poly(dG-dC).
    Nature. 1980 Nov 27;288(5789):413-4 PMID: 6253836
  11. Electron microscopy of the reactions of anti-poly A. poly U and anti-poly I. poly C antibodies with synthetic polynucleotide complexes and natural nucleic acids.
    Nucleic Acids Res. 1980 Apr 25;8(8):1805-22 PMID: 7433130
  12. The tetramer d(CpGpCpG) crystallizes as a left-handed double helix.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4016-20 PMID: 6933447
  13. Left-handed deoxyribonucleic acid double helix in solution.
    Biochemistry. 1981 Mar 31;20(7):2036-41 PMID: 7225372
  14. Double helical DNA: conformations, physical properties, and interactions with ligands.
    Annu Rev Biochem. 1981;50:997-1024 PMID: 7023371
  15. Changes in the helical repeat of poly(dG-m5dC) . poly(dG-m5dC) and poly(dG-dC) . poly(dG-dC) associated with the B-Z transition.
    Nature. 1981 Sep 17-23;293(5829):233-5 PMID: 7278982
  16. Antiserum to Z-DNA.
    FEBS Lett. 1981 Sep 14;132(1):45-8 PMID: 6170528
  17. The high salt form of poly(dG-dC).poly(dG-dC) is left-handed Z-DNA: Raman spectra of crystals and solutions.
    Nucleic Acids Res. 1981 Oct 24;9(20):5443-57 PMID: 7301594
  18. Structure of a B-DNA dodecamer. II. Influence of base sequence on helix structure.
    J Mol Biol. 1981 Jul 15;149(4):761-86 PMID: 6273591
  19. Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes.
    Nature. 1981 Dec 3;294(5840):417-22 PMID: 6796893
  20. The B goes to Z transition of poly(dG-dC) . poly(dG-dC) modified by some platinum derivatives.
    Nucleic Acids Res. 1981 Nov 11;9(21):5659-69 PMID: 7198223
  21. Solution structural studies of the A and Z forms of DNA.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6808-11 PMID: 6947255
  22. Comparison of poly(dG-dC).poly(dG-dC) conformations in oriented films and in solution.
    Proc Natl Acad Sci U S A. 1982 Jan;79(1):26-30 PMID: 6948301
  23. The molecular structure of d(ICpCpGpG), a fragment of right-handed double helical A-DNA.
    Nature. 1982 Jan 28;295(5847):294-9 PMID: 7057893
  24. Salt-induced co-operative conformational change of a synthetic DNA: equilibrium and kinetic studies with poly (dG-dC).
    J Mol Biol. 1972 Jun 28;67(3):375-96 PMID: 5045303
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
1149-53
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553181
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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