Home LiteratureArticle Details

PMID: 11894964 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12.

The EMBO journal ·Vol. 2 ·No. 3 ·1983-00-00 ·Pages 463-8

Kogoma T, von Meyenburg K

Abstract

The sdrA224 mutants of Escherichia coli K-12, capable of continued DNA replication in the absence of protein synthesis (stable DNA replication), tolerate inactivation of the dnaA gene by insertion of transposon Tn10. Furthermore, oriC, the origin of E. coli chromosome replication, can be deleted from the chromosome of sdrA mutants without loss of viability. The results suggest the presence of a second, normally repressed, initiation system for chromosome replication alternative to the 'normal' dnaA+ oriC+-dependent initiation mechanism.

MeSH Terms

Bacterial Proteins/genetics DNA Replication DNA, Bacterial/biosynthesis DNA-Binding Proteins/genetics Escherichia coli/genetics Genes, Viral Mutagenesis Replication Origin Transposases

Chemicals

Bacterial Proteins DNA, Bacterial DNA-Binding Proteins DnaA protein, Bacteria Transposases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kogoma T

Department of Microbiology, The Technical University of Denmark, Lyngby-Copenhagen.

von Meyenburg K

References (26)

26 references, click to expand

Characterization of the replication of Escherichia coli DNA in the absence of protein synthesis: stable DNA replication.
J Mol Biol. 1975 May 15;94(2):243-56 PMID: 1095769
Suppression of the DnaA phenotype by mutations in the rpoB cistron of ribonucleic acid polymerase in Salmonella typhimurium and Escherichia coli.
J Bacteriol. 1977 May;130(2):577-82 PMID: 400782
A novel Escherichia coli mutant capable of DNA replication in the absence of protein synthesis.
J Mol Biol. 1978 May 5;121(1):55-69 PMID: 351189
Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6.
Mol Gen Genet. 1978 Jun 14;162(2):121-37 PMID: 672900
Cloning of replication origins from the E. coli K12 chromosome.
Nature. 1978 Oct 12;275(5680):561-4 PMID: 357995
Nucleotide sequence of Escherichia coli K-12 replication origin.
Proc Natl Acad Sci U S A. 1979 Feb;76(2):575-9 PMID: 370832
Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.
Proc Natl Acad Sci U S A. 1979 Feb;76(2):580-4 PMID: 370833
Organization of structural and regulatory genes that mediate tetracycline resistance in transposon Tn10.
J Bacteriol. 1979 Jun;138(3):705-14 PMID: 378932
Origin of replication, oriC, of the Escherichia coli K12 chromosome: genetic mapping and minichromosome replication.
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:121-8 PMID: 383376
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
Characterization of the dnaA, gyrB and other genes in the dnaA region of the Escherichia coli chromosome on specialized transducing phages lambda tna.
Mol Gen Genet. 1979 Sep;175(2):135-44 PMID: 160000
Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.
Methods Enzymol. 1979;68:220-42 PMID: 94421
Induction of UV-resistant DNA replication in Escherichia coli: induced stable DNA replication as an SOS function.
Mol Gen Genet. 1979 Oct 2;176(1):1-9 PMID: 396443
Mutations in two unlinked genes are required to produce asparagine auxotrophy in Escherichia coli.
J Bacteriol. 1980 Apr;142(1):221-8 PMID: 6102983
Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl (DBM) - paper.
Nucleic Acids Res. 1980 Jan 25;8(2):299-317 PMID: 6158735
Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12.
J Bacteriol. 1981 Feb;145(2):904-13 PMID: 7007354
Identification of the dnaA and dnaN gene products of Escherichia coli.
Mol Gen Genet. 1980;180(2):267-73 PMID: 6258023
The dnaA gene product is not required during stable chromosome replication in Escherichia coli.
Mol Gen Genet. 1980;180(3):617-20 PMID: 7007826
Organization and transcription of the dnaA and dnaN genes of Escherichia coli.
Gene. 1981 Jan-Feb;13(1):47-55 PMID: 6453739
The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme of escherichia coli.
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5391-5 PMID: 6458041
DNA sequence organization of IS10-right of Tn10 and comparison with IS10-left.
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2608-12 PMID: 6283536
Suppressor mutations (rin) that specifically suppress the recA+ dependence of stable DNA replication in Escherichia coliK-12.
Mol Gen Genet. 1982;187(2):225-30 PMID: 6757658
Promoters of the atp operon coding for the membrane-bound ATP synthase of Escherichia coli mapped by Tn10 insertion mutations.
Mol Gen Genet. 1982;188(2):240-8 PMID: 6185823
The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12.
Nucleic Acids Res. 1982 Nov 25;10(22):7373-85 PMID: 6296774
The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli.
EMBO J. 1982;1(9):1043-8 PMID: 6329723
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736

Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1983-00-00

Pages

463-8

Language

English

Region

England

NLM ID

8208664

PMCID

PMC555155

Subset

Grants

NIGMS NIH HHS · GM22092 · United States

External Links

PubMed source PMC Full Text View journal details

Analysis Services

The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12.

Contact

WeChat Official Account