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PMID: 11907277 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The localization of the integral membrane protein Cps1p to the cell division site is dependent on the actomyosin ring and the septation-inducing network in Schizosaccharomyces pombe.

Molecular biology of the cell ·Vol. 13 ·No. 3 ·2002-03-00 ·Pages 989-1000

Liu J, Tang X, Wang H, Oliferenko S, Balasubramanian MK

Abstract

Schizosaccharomyces pombe cells divide by medial fission through the use of an actomyosin-based contractile ring. Constriction of the actomyosin ring is accompanied by the centripetal addition of new membranes and cell wall material. In this article, we characterize the mechanism responsible for the localization of Cps1p, a septum-synthesizing 1,3-beta-glucan synthase, to the division site during cytokinesis. We show that Cps1p is an integral membrane protein that localizes to the cell division site late in anaphase. Neither F-actin nor microtubules are essential for the initial assembly of Cps1p to the medial division site. F-actin, but not microtubules, is however important for the eventual incorporation of Cps1p into the actomyosin ring. Assembly of Cps1p into the cell division ring is also dependent on the septation-inducing network (SIN) proteins that regulate division septum formation after assembly of the actomyosin ring. Fluorescence-recovery after-photobleaching experiments reveal that Cps1p does not diffuse appreciably within the plasma membrane and is retained at the division site by a mechanism that does not depend on an intact F-actin cytoskeleton. We conclude that the actomyosin ring serves as a spatial cue for Cps1p localization, whereas the maintenance of Cps1p at the division site occurs by a novel F-actin- and microtubule-independent mechanism. Furthermore, we propose that the SIN proteins ensure localization of Cps1p at the appropriate point in the cell cycle.

MeSH Terms
Actins/metabolism Brefeldin A/pharmacology Cell Division/physiology DNA/metabolism Ethanol/pharmacology Glucosyltransferases/metabolism Green Fluorescent Proteins Indicators and Reagents/metabolism Luminescent Proteins/genetics,metabolism Membrane Proteins/metabolism Microtubules/metabolism Protein Synthesis Inhibitors/pharmacology Recombinant Fusion Proteins/genetics,metabolism Schizosaccharomyces/cytology,drug effects,physiology Schizosaccharomyces pombe Proteins/metabolism
Chemicals
Actins Indicators and Reagents Luminescent Proteins Membrane Proteins Protein Synthesis Inhibitors Recombinant Fusion Proteins Schizosaccharomyces pombe Proteins Green Fluorescent Proteins Brefeldin A Ethanol DNA Glucosyltransferases 1,3-beta-glucan synthase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Liu Jianhua
The Institute of Molecular Agrobiology, The National University of Singapore, Singapore 117604. [email protected]
Tang Xie
Wang Hongyan
Oliferenko Snezhana
Balasubramanian Mohan K
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.01-12-0581
Email
[email protected]
Published
2002-03-00
Pages
989-1000
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC99614
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.01-12-0581 PMC Full Text View journal details
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