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PMID: 11378383 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Completion of cytokinesis in C. elegans requires a brefeldin A-sensitive membrane accumulation at the cleavage furrow apex.

Current biology : CB ·Vol. 11 ·No. 10 ·2001-05-15 ·Pages 735-46

Skop AR, Bergmann D, Mohler WA, White JG

Abstract

The terminal phase of cytokinesis in eukaryotic cells involves breakage of the intercellular canal containing the spindle midzone and resealing of the daughter cells. Recent observations suggest that the spindle midzone is required for this process. In this study, we investigated the possibility that targeted secretion in the vicinity of the spindle midzone is required for the execution of the terminal phase of cytokinesis. We inhibited secretion in early C. elegans embryos by treatment with brefeldin A (BFA). Using 4D recordings of dividing cells, we showed that BFA induced stereotyped failures in the terminal phase of cytokinesis; although the furrow ingressed normally, after a few minutes the furrow completely regressed, even though spindle midzone and midbody microtubules appeared normal. In addition, using an FM1-43 membrane probe, we found that membrane accumulated locally at the apices of the late cleavage furrows that form the persisting intercellular canals between daughter cells. However, in BFA-treated embryos this membrane accumulation did not occur, which possibly accounts for the observed cleavage failures. We have shown that BFA disrupts the terminal phase of cytokinesis in the embryonic blastomeres of C. elegans. We observed that membrane accumulates at the apices of the late cleavage furrow by means of a BFA-sensitive mechanism. We suggest that this local membrane accumulation is necessary for the completion of cytokinesis and speculate that the spindle midzone region of animal cells is functionally equivalent to the phragmoplast of plants and acts to target secretion to the equatorial plane of a cleaving cell.

MeSH Terms
Animals Blastomeres/drug effects Brefeldin A/pharmacology Caenorhabditis elegans/embryology Cell Cycle/drug effects Cell Membrane/drug effects,metabolism Embryo, Nonmammalian/cytology Spindle Apparatus
Chemicals
Brefeldin A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Skop A R
Laboratory of Molecular Biology, University of Colorado, Boulder, CO 80309, USA.
Bergmann D
Mohler W A
White J G
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Article Info
Journal
Current biology : CB
Abbr.
Curr Biol
ISSN
0960-9822
DOI
10.1016/s0960-9822(01)00231-7
Published
2001-05-15
Pages
735-46
Language
English
Region
England
NLM ID
9107782
PMCID
PMC3733387
Subset
IM
Grants
NIGMS NIH HHS · F32 GM064159 · United States
NIGMS NIH HHS · R01 GM052454 · United States
NIGMS NIH HHS · R01 GM057583 · United States
NIGMS NIH HHS · GM-52454 · United States
External Links
PubMed source DOI: 10.1016/s0960-9822(01)00231-7 PMC Full Text View journal details
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