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PMID: 1194239 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Detection and quantitation of Staphylococcus aureus penicillinase plasmid deoxyribonucleic acid by reassociation kinetics.

Journal of bacteriology ·Vol. 124 ·No. 3 ·1975-12-00 ·Pages 1417-23

Rush M, Novick R, DeLap R

Abstract

The quantity of penicillinase plasmid deoxyribonucleic acid (DNA) in various strains of Staphylococcus aureus has been determined by DNA-DNA reassociation kinetics. Specifically, 32P- or 125I-labeled denatured probes of purified plasmid DNA were reassociated in the presence of denatured DNAs isolated from the bacterial strains in question. The number of plasmid copies per cell was calculated from the effect of the latter nucleic acid samples on the reassociation rate of the radiolabeled probe. Among the S. aureus strains examined were monoplasmid, diplasmid and replication-defective representatives, and the effect of temperature on wild-type plasmid content was also investigated.

MeSH Terms
Base Sequence DNA, Bacterial/analysis Extrachromosomal Inheritance Nucleic Acid Denaturation Nucleic Acid Hybridization Penicillinase/biosynthesis Plasmids Staphylococcus aureus/analysis,enzymology Temperature
Chemicals
DNA, Bacterial Penicillinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rush M
Novick R
DeLap R
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.124.3.1417-1423.1975
Published
1975-12-00
Pages
1417-23
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC236055
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.124.3.1417-1423.1975 PMC Full Text View journal details
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