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PMID: 119896 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Chromosomal mutations causing resistance to tetracycline in Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 177 ·No. 1 ·1979-00-00 ·Pages 23-9

Williams G, Smith I

Abstract

We have isolated, after ethylmethanesulfonate mutagenesis, several chromosomal mutations causing resistance to tetracycline in Bacillus subtilis. These mutations fall into two classics, tetA and tetB. 30 S ribosomal protein S10 shows an altered mobility on two-dimensional acrylamide gels in cells bearing the former type of mutation. Ribosomes from these cells show elevated levels of resistance to tetracycline in vitro as measured by polyuridine dependent polyphenylalanine synthesis. The tetA locus maps adjacent to the tuf gene in the B. subtilis ribosomal protein gene cluster. Cells with the tetB mutation do not show any altered ribosomal protein, and their ribosomes are as sensitive, in vitro, to tetracycline as ribosomes isolated from wild type cells. The tetB mutation has been mapped proximal to cysA14.

MeSH Terms
Bacillus subtilis/drug effects,genetics Chromosome Mapping Chromosomes, Bacterial/ultrastructure Drug Resistance, Microbial Ethyl Methanesulfonate Mutation Phenotype Ribosomal Proteins/genetics Tetracycline/pharmacology Transformation, Bacterial
Chemicals
Ribosomal Proteins Ethyl Methanesulfonate Tetracycline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Williams G
Smith I
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24 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267249
Published
1979-00-00
Pages
23-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267249
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