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PMID: 12006659 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Caenorhabditis elegans inositol 5-phosphatase homolog negatively regulates inositol 1,4,5-triphosphate signaling in ovulation.

Molecular biology of the cell ·Vol. 13 ·No. 5 ·2002-05-00 ·Pages 1641-51

Bui YK, Sternberg PW

Abstract

Ovulation in Caenorhabditis elegans requires inositol 1,4,5-triphosphate (IP(3)) signaling activated by the epidermal growth factor (EGF)-receptor homolog LET-23. We generated a deletion mutant of a type I 5-phosphatase, ipp-5, and found a novel ovulation phenotype whereby the spermatheca hyperextends to engulf two oocytes per ovulation cycle. The temporal and spatial expression of IPP-5 is consistent with its proposed inhibition of IP(3) signaling in the adult spermatheca. ipp-5 acts downstream of let-23, and interacts with let-23-mediated IP(3) signaling pathway genes. We infer that IPP-5 negatively regulates IP(3) signaling to ensure proper spermathecal contraction.

MeSH Terms
Amino Acid Sequence Animals Caenorhabditis elegans/enzymology,genetics,metabolism Caenorhabditis elegans Proteins Epidermal Growth Factor ErbB Receptors/metabolism Helminth Proteins/metabolism Inositol 1,4,5-Trisphosphate/metabolism Inositol Polyphosphate 5-Phosphatases Molecular Sequence Data Ovulation/metabolism Phosphoric Monoester Hydrolases/genetics,metabolism Sequence Alignment Sequence Analysis, DNA Sequence Deletion Signal Transduction/physiology
Chemicals
Caenorhabditis elegans Proteins Helminth Proteins Lin-3 protein, C elegans Epidermal Growth Factor Inositol 1,4,5-Trisphosphate ErbB Receptors let-23 protein, C elegans Phosphoric Monoester Hydrolases Inositol Polyphosphate 5-Phosphatases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bui Yen Kim
Howard Hughes Medical Institute and Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Sternberg Paul W
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.02-01-0008
Published
2002-05-00
Pages
1641-51
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC111133
Subset
IM
Grants
NIGMS NIH HHS · T32 GM007737 · United States
NIGMS NIH HHS · 5T32GM07737 · United States
External Links
PubMed source DOI: 10.1091/mbc.02-01-0008 PMC Full Text View journal details
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