Home LiteratureArticle Details
PMID: 1201686 Published · ppublish English Journal Article

Independent replication of the ribosomal RNA genes in the polytrophic-meroistic ovaries of Calliphora erythrocephala, Drosophila hydei, and Sarcophaga barbata.

Chromosoma ·Vol. 53 ·No. 2 ·1975-11-24 ·Pages 131-40

Renkawitz R, Kunz W

Abstract

By filter saturation hybridizations the ribosomal (r)DNA contents of the ovaries Calliphora erythrocephala, Drosophila hydei, and Sarcophaga barbata have been measured in comparison to the rDNA percentages of their diploid brains. The measurements of the ovarian rDNA have been carried out on ovaries where the nurse cells in the distal egg chamber of the ovarioles had reached their highest ploidy level. The diploid rDNA content of each of the respective species was chosen as a 100% standard and the rDNA amounts of the ovaries were related to this 100% level. The results show that the ovaries of C. erythrocephala contain 135% rDNA whereas the rDNA contents in the ovaries of D. hydei and S. barbata are only 51% and 47%, respectively. Measurements carried out on isolated nuclei of the nurse cells and follicle cells in D. hydei show that both have a reduced rDNA content in comparison to the brains (45% and 70%, respectively). The data are discussed in relation to the problem of an rDNA amplification in the germ cells and an rDNA underreplication in polyploid nuclei.

MeSH Terms
Animals Brain/metabolism DNA/analysis DNA Replication Diploidy Diptera/metabolism Drosophila/metabolism Female Genes Nucleic Acid Hybridization Oocytes/metabolism Polyploidy RNA, Ribosomal/biosynthesis
Chemicals
RNA, Ribosomal DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Renkawitz R
Kunz W
References (30)
30 references, click to expand
  1. Ribosomal RNA genes in salivary gland and ovary of Rhynchosclara angelae.
    J Cell Biol. 1972 Aug;54(2):421-6 PMID: 5040866
  2. [Structure and function of oocyte chromosomes nucleoli and as well as the extra DNA during oogenesis in panoistic and meroistic insects].
    Chromosoma. 1967;23(2):214-54 PMID: 4875166
  3. Rapid equilibrium isopycnic CsC1 gradients.
    Biochim Biophys Acta. 1969 Mar 18;179(1):136-44 PMID: 4894516
  4. Amplication of ribosomal cistrons in the heterochromatin of acheta.
    Genetics. 1969;61(1):Suppl:145-59 PMID: 5345377
  5. [Analysis of the RNA fractions of mouse liver: comparison of the extraction methods of Kirby and Georgiev].
    Z Naturforsch B. 1971 Mar;26(3):235-43 PMID: 4396548
  6. Multiple nucleoli and enhanced nucleolar activity in the nurse cells of the insect ovary.
    Chromosoma. 1969;27(2):178-97 PMID: 5364941
  7. Regulation of ribosomal RNA synthesis and its bearing on the bobbed phenotype in Drosophila melanogaster.
    Dev Biol. 1970 Jul;22(3):495-512 PMID: 5463671
  8. Fine structural changes in theDrosophila oocyte nucleus during a short period of RNA synthesis : An autoradiographic and ultrastructural study of RNA synthesis in the oocyte nucleus ofDrosophila.
    Wilhelm Roux Arch Entwickl Mech Org. 1970 Mar;165(1):8-25 PMID: 28304458
  9. Variations in the number of the Y chromosomal rRNA genes in Drosophila hydei.
    Genetics. 1976 Jan;82(1):25-34 PMID: 814041
  10. Gene amplification in the oocytes of Dytiscid water beetles.
    Chromosoma. 1969;26(2):169-87 PMID: 5800663
  11. Size, conformation and purity of chloroplast DNA of some higher plants.
    Biochim Biophys Acta. 1975 Jan 20;378(2):305-17 PMID: 1092350
  12. Differential synthesis of the genes for ribosomal RNA during amphibian oögenesis.
    Proc Natl Acad Sci U S A. 1968 Jun;60(2):553-60 PMID: 5248812
  13. Mass preparation of nuclei from the larval salivary glands of Drosophila hydei.
    J Cell Biol. 1968 Aug;38(2):369-76 PMID: 5664209
  14. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.
    J Mol Biol. 1965 Feb;11:373-90 PMID: 14290352
  15. Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
    Science. 1968 Apr 19;160(3825):272-80 PMID: 4867987
  16. Under-replication of ribosomal cistrons in polytene chromosomes of Rhynchosciara.
    J Cell Biol. 1974 Jul;62(1):215-22 PMID: 4407049
  17. Localization of amplified ribosomal DNA in the oocyte of Xenopus laevis.
    Biochim Biophys Acta. 1968 Aug 23;166(1):274-6 PMID: 5698061
  18. Independent control of ribosomal gene replication in polytene chromosomes of Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1359-63 PMID: 4197090
  19. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
    Biochem J. 1967 Jan;102(1):251-7 PMID: 5339944
  20. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
    J Mol Biol. 1965 Jul;12(3):829-42 PMID: 4955314
  21. [Metabolic transport in the ovary of Pyrrhocoris apertus L. Autoradiographic studies on the metabolic transport from nutritive cells to the oocyte in the firebug Pyrrhocoris apertus L. (Heteroptera)].
    Z Zellforsch Mikrosk Anat. 1972;123(3):395-410 PMID: 5008777
  22. Preparative gel electrophoresis of liver polyribosomal RNA.
    Anal Biochem. 1972 Feb;45(2):645-52 PMID: 5060612
  23. Differential replication of satellite DNA in polyploid tissues of Drosophila virilis.
    Chromosoma. 1975;50(2):175-9 PMID: 1132286
  24. Different satellite deoxyribonucleic acids of guinea pig and ox.
    Biochemistry. 1970 Mar 31;9(7):1565-71 PMID: 5436146
  25. [Study of a non deleted bobbed mutant (author's transl)].
    Mol Gen Genet. 1974;135(3):213-29 PMID: 4218302
  26. Reduced polyteny of ribosomal RNA cistrons in giant chromosomes of Drosophila hydei.
    Nat New Biol. 1971 Sep 15;233(37):70-2 PMID: 5286999
  27. A massive system of microtubules associated with cytoplasmic movement in telotrophic ovarioles.
    J Cell Sci. 1970 Mar;6(2):431-49 PMID: 5463247
  28. Underreplication of satellite dnas in polyploid ovarian tissue of Drosophila virilis.
    Chromosoma. 1975;49(4):375-82 PMID: 1132282
  29. [RNA-Synthesis in telotrophic meroistic ovarioles of dysdercus intermedius DIST. (heteroptera, pyrrhoc.)].
    Wilhelm Roux Arch Entwickl Mech Org. 1973 Jun;172(2):83-130 PMID: 28304586
  30. [Synthesis, intercellular transport, and analysis of ribonucleic acids in the ovum of the housefly Musca domestica].
    J Cell Biol. 1963 Feb;16:436-40 PMID: 13968182
Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00333041
Published
1975-11-24
Pages
131-40
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00333041 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2