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PMID: 12022229 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast Pescadillo is required for multiple activities during 60S ribosomal subunit synthesis.

RNA (New York, N.Y.) ·Vol. 8 ·No. 5 ·2002-05-00 ·Pages 626-36

Oeffinger M, Leung A, Lamond A, Tollervey D, Lueng A

Abstract

The Pescadillo protein was identified via a developmental defect and implicated in cell cycle progression. Here we report that human Pescadillo and its yeast homolog (Yph1p or Nop7p) are localized to the nucleolus. Depletion of Nop7p leads to nuclear accumulation of pre-60S particles, indicating a defect in subunit export, and it interacts genetically with a tagged form of the ribosomal protein Rpl25p, consistent with a role in subunit assembly. Two pre-rRNA processing pathways generate alternative forms of the 5.8S rRNA, designated 5.8S(L) and 5.8Ss. In cells depleted for Nop7p, the 27SA3 pre-rRNA accumulated, whereas later processing intermediates and the mature 5.8Ss rRNA were depleted. Less depletion was seen for the 5.8S(L) pathway. TAP-tagged Nop7p coprecipitated precursors to both 5.8S(L) and 5.8Ss but not the mature rRNAs. We conclude that Nop7p is required for efficient exonucleolytic processing of the 27SA3 pre-rRNA and has additional functions in 60S subunit assembly and transport. Nop7p is a component of at least three different pre-60S particles, and we propose that it carries out distinct functions in each of these complexes.

MeSH Terms
Base Sequence Cell Nucleolus/metabolism Humans In Vitro Techniques Models, Biological Nuclear Proteins/metabolism Proteins/metabolism RNA Precursors/genetics,metabolism RNA Processing, Post-Transcriptional RNA, Fungal/genetics,metabolism Recombinant Fusion Proteins/metabolism Ribosomes/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/metabolism
Chemicals
NOP7 protein, S cerevisiae Nuclear Proteins Proteins RNA Precursors RNA, Fungal Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Oeffinger Marlene
Wellcome Trust Centre for Cell Biology, University of Edinburgh, United Kingdom.
Leung Anthony
Lamond Angus
Tollervey David
Lueng Anthony
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1017/s1355838202020022
Published
2002-05-00
Pages
626-36
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1370283
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1017/s1355838202020022 PMC Full Text View journal details
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