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PMID: 8039506 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synthetic lethality with fibrillarin identifies NOP77p, a nucleolar protein required for pre-rRNA processing and modification.

The EMBO journal ·Vol. 13 ·No. 13 ·1994-07-01 ·Pages 3136-48

Bergès T, Petfalski E, Tollervey D, Hurt EC

Abstract

The nucleolar protein fibrillarin (encoded by the NOP1 gene in yeast), is required for many post-transcriptional steps in yeast ribosome synthesis. A screen for mutations showing synthetic lethality with a temperature sensitive nop1-5 allele led to the identification of the NOP77 gene. NOP77 is essential for viability and encodes a nucleolar protein with a predicted molecular weight of 77 kDa. Depletion of NOP77p impairs both the processing and methylation of the pre-rRNA. The processing defect is greatest for the pathway leading to 25S rRNA synthesis, and is distinctly different from that observed for mutations in other nucleolar components. NOP77p contains three canonical RNA recognition motifs (RRMs), suggesting that it is an RNA binding protein. The NOP77 allele which complements the synthetic lethal nop1 strains has an alanine at position 308, predicted to lie in helix alpha 1 of RRM3, whereas the non-complementing nop77-1 allele contains a proline at the corresponding position. We propose that NOP77p mediates specific interactions between NOP1p and the pre-rRNA.

Related Genes
NOP77
MeSH Terms
Amino Acid Sequence Base Sequence Chromosomal Proteins, Non-Histone/genetics Cloning, Molecular DNA, Fungal Fungal Proteins/genetics,metabolism Genes, Fungal Genes, Lethal Methylation Molecular Sequence Data Mutation Nuclear Proteins/chemistry,genetics,metabolism,physiology RNA Precursors/metabolism RNA Processing, Post-Transcriptional RNA, Fungal/metabolism RNA, Ribosomal/metabolism RNA-Binding Proteins/chemistry,genetics,physiology Ribonucleoproteins, Small Nuclear/metabolism Ribonucleoproteins, Small Nucleolar Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins
Chemicals
Chromosomal Proteins, Non-Histone DNA, Fungal Fungal Proteins NOP1 protein, S cerevisiae NOP4 protein, S cerevisiae Nuclear Proteins RNA Precursors RNA, Fungal RNA, Ribosomal RNA-Binding Proteins Ribonucleoproteins, Small Nuclear Ribonucleoproteins, Small Nucleolar Saccharomyces cerevisiae Proteins fibrillarin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bergès T
EMBL, Heidelberg, Germany.
Petfalski E
Tollervey D
Hurt E C
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48 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-07-01
Pages
3136-48
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395205
Subset
IM
Databases
GENBANK
X76245
External Links
PubMed source PMC Full Text View journal details
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