cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 3 ·1991-02-01 ·Pages 931-5

Aris JP, Blobel G

Abstract

We have isolated a 1.1-kilobase cDNA clone that encodes human fibrillarin by screening a hepatoma library in parallel with DNA probes derived from the fibrillarin genes of Saccharomyces cerevisiae (NOP1) and Xenopus laevis. RNA blot analysis indicates that the corresponding mRNA is approximately 1300 nucleotides in length. Human fibrillarin expressed in vitro migrates on SDS gels as a 36-kDa protein that is specifically immunoprecipitated by antisera from humans with scleroderma autoimmune disease. Human fibrillarin contains an amino-terminal repetitive domain approximately 75-80 amino acids in length that is rich in glycine and arginine residues and is similar to amino-terminal domains in the yeast and Xenopus fibrillarins. The occurrence of a putative RNA-binding domain and an RNP consensus sequence within the protein is consistent with the association of fibrillarin with small nucleolar RNAs. Protein sequence alignments show that 67% of amino acids from human fibrillarin are identical to those in yeast fibrillarin and that 81% are identical to those in Xenopus fibrillarin. This identity suggests the evolutionary conservation of an important function early in the pathway for ribosome biosynthesis.

MeSH Terms

Amino Acid Sequence Animals Autoantibodies/immunology Base Sequence Carcinoma, Hepatocellular Cell Line Chromosomal Proteins, Non-Histone/genetics,immunology Cloning, Molecular DNA, Neoplasm/genetics,isolation & purification Gene Library HeLa Cells/metabolism Humans Liver Neoplasms Molecular Sequence Data Rats Sequence Homology, Nucleic Acid Xenopus laevis

Chemicals

Autoantibodies Chromosomal Proteins, Non-Histone DNA, Neoplasm fibrillarin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Aris J P

Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021.

Blobel G

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31 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.88.3.931

Published

1991-02-01

Pages

931-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC50928

Subset

Databases

GENBANK

M59849, M73267, M73268, M73269, S60069, S60071, S60073, S60076, S60079, S60081

External Links

PubMed source DOI: 10.1073/pnas.88.3.931 PMC Full Text View journal details

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cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera.

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