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PMID: 1825809 Published · ppublish English Journal Article

The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast.

The EMBO journal ·Vol. 10 ·No. 3 ·1991-03-00 ·Pages 573-83

Tollervey D, Lehtonen H, Carmo-Fonseca M, Hurt EC

Abstract

The yeast snoRNP protein, NOP1, is structurally and functionally homologous to vertebrate fibrillarin and is essential for viability. A conditionally lethal allele was constructed by placing NOP1 expression under the control of a GAL promoter. Growth on glucose medium results in the depletion of NOP1 over several generations, during which cell growth is progressively impaired. Pulse labelling of proteins shows that NOP1 depleted strains are greatly impaired in the production of cytoplasmic ribosomes, and they have a reduced level of rRNA. Northern hybridization and pulse-chase labelling of pre-rRNA show a progressive impairment of all pre-rRNA processing steps. The pathway leading to 18S rRNA is particularly affected. Methylation of pre-rRNA is concomitantly impaired and unmethylated pre-rRNA accumulates and is not processed over long periods. NOP1 depletion does not prevent the accumulation of seven snoRNAs tested including U3; the levels of two species, U14 and snR190, decline. The snoRNAs synthesized in the absence of NOP1 retain TMG cap structures. Subnuclear fractionation and immunocytochemistry indicate that they continue to be localized in the nucleolus.

MeSH Terms
Base Sequence Blotting, Northern Chromosomal Proteins, Non-Histone/metabolism DNA, Ribosomal/genetics Fungal Proteins/genetics,metabolism Genes, Fungal Genes, Lethal Molecular Sequence Data Nuclear Proteins/genetics,metabolism Nucleic Acid Hybridization Oligonucleotide Probes Promoter Regions, Genetic RNA Precursors/genetics RNA, Ribosomal/genetics RNA, Small Nuclear/genetics,isolation & purification Ribonucleoproteins/metabolism Ribonucleoproteins, Small Nuclear Ribonucleoproteins, Small Nucleolar Ribosomes/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins
Chemicals
Chromosomal Proteins, Non-Histone DNA, Ribosomal Fungal Proteins NOP1 protein, S cerevisiae Nuclear Proteins Oligonucleotide Probes RNA Precursors RNA, Ribosomal RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear Ribonucleoproteins, Small Nucleolar Saccharomyces cerevisiae Proteins fibrillarin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tollervey D
EMBL, Heidelberg, Germany.
Lehtonen H
Carmo-Fonseca M
Hurt E C
References (41)
41 references, click to expand
  1. Identification of a yeast snRNP protein and detection of snRNP-snRNP interactions.
    Cell. 1987 Dec 24;51(6):1019-26 PMID: 2961458
  2. The effects of methionine deprivation on ribosome synthesis in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1527-34 PMID: 5237886
  3. Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
    Cell. 1986 Sep 12;46(6):905-11 PMID: 2944599
  4. A subset of yeast snRNA's contains functional binding sites for the highly conserved Sm antigen.
    Science. 1987 Jan 16;235(4786):328-31 PMID: 2948278
  5. Structural analysis of the human U3 ribonucleoprotein particle reveal a conserved sequence available for base pairing with pre-rRNA.
    Mol Cell Biol. 1987 Aug;7(8):2899-913 PMID: 2959855
  6. Fungal small nuclear ribonucleoproteins share properties with plant and vertebrate U-snRNPs.
    EMBO J. 1987 Feb;6(2):469-76 PMID: 2953599
  7. Low molecular weight RNA associated with 28 s nucleolar RNA.
    J Mol Biol. 1970 Feb 14;47(3):505-15 PMID: 5418169
  8. SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs.
    J Cell Biol. 1990 Nov;111(5 Pt 1):1741-51 PMID: 2121740
  9. Nuclear function and organization: the potential of immunochemical approaches.
    Int Rev Cytol. 1988;110:27-92 PMID: 3053500
  10. Nucleolar and nuclear envelope proteins of the yeast Saccharomyces cerevisiae.
    Eur J Cell Biol. 1988 Aug;46(3):554-63 PMID: 3053175
  11. Deletion of a yeast small nuclear RNA gene impairs growth.
    EMBO J. 1985 Dec 30;4(13B):3873-8 PMID: 3004976
  12. U3 small nuclear RNA can be psoralen-cross-linked in vivo to the 5' external transcribed spacer of pre-ribosomal-RNA.
    Proc Natl Acad Sci U S A. 1989 Sep;86(17):6523-7 PMID: 2771939
  13. Characterization of an SNR gene locus in Saccharomyces cerevisiae that specifies both dispensible and essential small nuclear RNAs.
    Mol Cell Biol. 1988 Aug;8(8):3282-90 PMID: 2850487
  14. Sequence and genetic analysis of a dispensible 189 nucleotide snRNA from Saccharomyces cerevisiae.
    Nucleic Acids Res. 1988 Jun 24;16(12):5587-601 PMID: 3290853
  15. Purification and partial characterization of a nucleolar scleroderma antigen (Mr = 34,000; pI, 8.5) rich in NG,NG-dimethylarginine.
    J Biol Chem. 1985 Nov 15;260(26):14304-10 PMID: 2414294
  16. Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
    Cell. 1985 Jan;40(1):111-8 PMID: 2578319
  17. U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
    EMBO J. 1989 Oct;8(10):3113-9 PMID: 2531075
  18. PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle.
    Mol Cell Biol. 1989 Sep;9(9):3710-9 PMID: 2528687
  19. Isolation and characterization of yeast ribosomal RNA precursors and preribosomes.
    Methods Enzymol. 1989;180:96-109 PMID: 2693913
  20. A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability.
    EMBO J. 1989 Dec 20;8(13):4015-24 PMID: 2686980
  21. The 5' end of U3 snRNA can be crosslinked in vivo to the external transcribed spacer of rat ribosomal RNA precursors.
    J Mol Biol. 1989 Dec 5;210(3):497-512 PMID: 2614831
  22. Genetic analysis of small nuclear RNAs in Saccharomyces cerevisiae: viable sextuple mutant.
    Mol Cell Biol. 1988 Aug;8(8):3150-9 PMID: 2905424
  23. A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA.
    EMBO J. 1987 Dec 20;6(13):4169-75 PMID: 3327689
  24. Saccharomyces cerevisiae has a U1-like small nuclear RNA with unexpected properties.
    Science. 1987 Sep 18;237(4821):1484-7 PMID: 3306922
  25. The yeast homologue of U3 snRNA.
    EMBO J. 1987 Jul;6(7):2145-55 PMID: 3308452
  26. The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing.
    Cell. 1990 Mar 23;60(6):897-908 PMID: 2156625
  27. Unexpected flexibility in an evolutionarily conserved protein-RNA interaction: genetic analysis of the Sm binding site.
    EMBO J. 1990 Aug;9(8):2555-61 PMID: 2142451
  28. The role of small nucleolar ribonucleoproteins in ribosome synthesis.
    Mol Biol Rep. 1990;14(2-3):103-6 PMID: 2141891
  29. Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease.
    Mol Cell Biol. 1990 Jan;10(1):430-4 PMID: 2136767
  30. Differential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus.
    Mol Cell Biol. 1990 Jul;10(7):3524-34 PMID: 2192258
  31. Functional and dynamic aspects of the mammalian nucleolus.
    Bioessays. 1990 Jan;12(1):14-21 PMID: 2181998
  32. Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae.
    Mol Cell Biol. 1990 Mar;10(3):1145-52 PMID: 2406561
  33. Isolation and sequencing of NOP1. A yeast gene encoding a nucleolar protein homologous to a human autoimmune antigen.
    J Biol Chem. 1990 Feb 5;265(4):2209-15 PMID: 2298745
  34. A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7410-4 PMID: 6760197
  35. A U4-like small nuclear RNA is dispensable in yeast.
    Cell. 1983 Dec;35(3 Pt 2):753-62 PMID: 6197184
  36. The structure of mammalian small nuclear ribonucleoproteins. Identification of multiple protein components reactive with anti-(U1)ribonucleoprotein and anti-Sm autoantibodies.
    J Biol Chem. 1984 May 10;259(9):5907-14 PMID: 6232278
  37. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  38. Isolation and characterization of rabbit anti-m3 2,2,7G antibodies.
    Nucleic Acids Res. 1982 Nov 25;10(22):7103-13 PMID: 7155893
  39. Identification of NG, NG-dimethylarginine in a nuclear protein from the lower eukaryote physarum polycephalum homologous to the major proteins of mammalian 40S ribonucleoprotein particles.
    Biochem Biophys Res Commun. 1977 Jan 24;74(2):621-9 PMID: 556939
  40. Nucleolar function of the dense crescent in the yeast nucleus. A biochemical and ultrastructural study.
    Exp Cell Res. 1973 Aug;80(2):313-21 PMID: 4745379
  41. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera.
    Biol Cell. 1985;54(2):123-33 PMID: 2933102
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-03-00
Pages
573-83
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452687
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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