Home LiteratureArticle Details
PMID: 2192258 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Differential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus.

Molecular and cellular biology ·Vol. 10 ·No. 7 ·1990-07-00 ·Pages 3524-34

Potashkin JA, Derby RJ, Spector DL

Abstract

The yeast cell nucleus has previously been shown to be divided into two regions by a variety of microscopic approaches. We used antibodies specific for the 2,2,7-trimethylguanosine cap structure of small nuclear ribonucleic acids (snRNAs) and for a protein component of small nuclear ribonucleoprotein particles to identify the distribution of small nuclear ribonucleoprotein particles within the yeast cell nucleus. These studies were performed with the fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae. By using immunofluorescence microscopy and immunoelectron microscopy, most of the abundant snRNAs were localized to the portion of the nucleus which has heretofore been referred to as the nucleolus. This distribution of snRNAs is different from that found in mammalian cells and suggests that the nucleolar portion of the yeast nucleus contains functional domains in addition to those associated with RNA polymerase I activity.

MeSH Terms
Antibodies Cell Nucleus/metabolism,ultrastructure Fluorescent Antibody Technique Guanosine/analogs & derivatives,analysis Immunohistochemistry Microscopy, Electron RNA Precursors/genetics RNA Processing, Post-Transcriptional RNA, Small Nuclear/analysis,genetics Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Schizosaccharomyces/genetics,metabolism,ultrastructure Spheroplasts/metabolism,ultrastructure
Chemicals
Antibodies RNA Precursors RNA, Small Nuclear Guanosine N(2),N(2),7-trimethylguanosine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Potashkin J A
Cold Spring Harbor Laboratory, New York 11724.
Derby R J
Spector D L
References (50)
50 references, click to expand
  1. The morphological relationship in electron microscopy between NOR-silver proteins and intranucleolar chromatin.
    Chromosoma. 1982;85(4):461-73 PMID: 6181942
  2. A fiber apparatus in the nucleus of the yeast cell.
    J Cell Biol. 1966 Apr;29(1):129-51 PMID: 5331666
  3. Yeast contains small nuclear RNAs encoded by single copy genes.
    Cell. 1983 Dec;35(3 Pt 2):743-51 PMID: 6197183
  4. Immunoelectron microscopic localization of snRNPs.
    Biol Cell. 1983;49(1):1-10 PMID: 6230127
  5. Further evidence that phosphoprotein C23 (110 kD/pI 5.1) is the nucleolar silver staining protein.
    Exp Cell Res. 1984 May;152(1):260-5 PMID: 6201373
  6. Nucleolar structure.
    Int Rev Cytol. 1984;87:107-58 PMID: 6201455
  7. 5'-Terminal caps of snRNAs are reactive with antibodies specific for 2,2,7-trimethylguanosine in whole cells and nuclear matrices. Double-label immunofluorescent studies with anti-m3G antibodies and with anti-RNP and anti-Sm autoantibodies.
    Exp Cell Res. 1984 Oct;154(2):548-60 PMID: 6207038
  8. Colocalization of U1 and U2 small nuclear RNPs by immunocytochemistry.
    Biol Cell. 1984;51(1):109-12 PMID: 6207877
  9. Multiple states of U3 RNA in Novikoff hepatoma nucleoli.
    Biochemistry. 1984 Nov 6;23(23):5421-5 PMID: 6210104
  10. Anchorage of the nucleolus in the pore complex-lamina by a DNA-bearing structure masked in situ in rat liver nuclei.
    Biol Cell. 1984;52(2):91-101 PMID: 6241495
  11. Low molecular weight RNA associated with 28 s nucleolar RNA.
    J Mol Biol. 1970 Feb 14;47(3):505-15 PMID: 5418169
  12. Biochemical and electron microscopic study of isolated yeast nuclei.
    Exp Cell Res. 1970 May;60(2):148-56 PMID: 5424309
  13. Mitosis in the fission yeast Schizosaccharomyces pombe: a comparative study with light and electron microscopy.
    J Cell Sci. 1971 Sep;9(2):475-507 PMID: 4108061
  14. Electron microscopic autoradiographic study of RNA synthesis in yeast nucleus.
    Exp Cell Res. 1972 Jan;70(1):140-4 PMID: 5008396
  15. Mixed connective tissue disease--an apparently distinct rheumatic disease syndrome associated with a specific antibody to an extractable nuclear antigen (ENA).
    Am J Med. 1972 Feb;52(2):148-59 PMID: 4621694
  16. Nucleolar function of the dense crescent in the yeast nucleus. A biochemical and ultrastructural study.
    Exp Cell Res. 1973 Aug;80(2):313-21 PMID: 4745379
  17. Differential staining of the satellite regions of human acrocentric chromosomes.
    Experientia. 1975 Feb 15;31(2):260-2 PMID: 46207
  18. Chromatin behaviour during the mitotic cell cycle of Saccharomyces cerevisiae.
    J Cell Sci. 1977 Apr;24:81-93 PMID: 330549
  19. The nature of the Ag-staining of nucleolus organizer regions. Electron- and light-microscopic studies on human cells in interphase, mitosis, and meiosis.
    Cytogenet Cell Genet. 1978;20(1-6):24-39 PMID: 77208
  20. Onset of nucleolus organizer activity in early mouse embryogenesis and evidence for its regulation.
    Exp Cell Res. 1978 Jul;114(2):263-8 PMID: 567122
  21. Silver staining of NORs in electron microscopy.
    Exp Cell Res. 1979 Oct 15;123(2):449-52 PMID: 91530
  22. Identification of a silver binding protein associated with the cytological silver staining of actively transcribing nucleolar regions.
    Cell Biol Int Rep. 1979 Oct;3(7):615-22 PMID: 93980
  23. Evidence from studies on segregated nucleoli that nucleolar silver staining proteins C23 and B23 are in the fibrillar component.
    Exp Cell Res. 1980 Jun;127(2):285-91 PMID: 6155282
  24. Ultrastructural localization of Ag-NOR stained proteins in the nucleolus during the cell cycle and in other nucleolar structures.
    Chromosoma. 1980;79(3):349-62 PMID: 6156811
  25. Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2737-41 PMID: 6789322
  26. Patterns of silver staining on NORs of prematurely condensed muntjac chromosomes following RNA inhibition.
    Exp Cell Res. 1981 Oct;135(2):373-8 PMID: 6171437
  27. Argyrophilic nuclear and nucleolar proteins of Xenopus laevis oocytes identified by gel electrophoresis.
    Exp Cell Res. 1982 Feb;137(2):341-51 PMID: 6173244
  28. Alterations in chromatin conformation are accompanied by reorganization of nonchromatin domains that contain U-snRNP protein p28 and nuclear protein p107.
    J Cell Biol. 1985 Aug;101(2):560-7 PMID: 2991302
  29. Nucleologenesis: composition and fate of prenucleolar bodies.
    Chromosoma. 1985;92(5):330-6 PMID: 3902398
  30. Purification and partial characterization of a nucleolar scleroderma antigen (Mr = 34,000; pI, 8.5) rich in NG,NG-dimethylarginine.
    J Biol Chem. 1985 Nov 15;260(26):14304-10 PMID: 2414294
  31. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera.
    Biol Cell. 1985;54(2):123-33 PMID: 2933102
  32. Intranuclear localization of snRNP antigens.
    J Cell Biol. 1986 Jan;102(1):137-44 PMID: 2934400
  33. Mitosis in the fission yeast Schizosaccharomyces pombe as revealed by freeze-substitution electron microscopy.
    J Cell Sci. 1986 Feb;80:253-68 PMID: 3522614
  34. The nucleolus and the nucleolar organizer regions.
    Biol Cell. 1986;56(3):189-205 PMID: 2943350
  35. Small nuclear RNAs from Saccharomyces cerevisiae: unexpected diversity in abundance, size, and molecular complexity.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8097-101 PMID: 3534883
  36. Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins.
    J Cell Biol. 1986 Dec;103(6 Pt 1):2103-12 PMID: 3536958
  37. A monoclonal anti-double stranded DNA antibody from an autoimmune MRL/Mp-lpr/lpr mouse: specificity and idiotype in serum immunoglobulins.
    Immunol Lett. 1986 Nov 17;14(1):53-8 PMID: 3492443
  38. Monoclonal autoantibody from a (New Zealand black x New Zealand white)F1 mouse and some human scleroderma sera target an Mr 34,000 nucleolar protein of the U3 RNP particle.
    Arthritis Rheum. 1987 Jul;30(7):793-800 PMID: 2441711
  39. Distribution of the 70K U1 RNA-associated protein during interphase and mitosis. Correlation with other U RNP particles and proteins of the nuclear matrix.
    J Cell Sci. 1986 Dec;86:173-90 PMID: 2958489
  40. Structural analysis of the human U3 ribonucleoprotein particle reveal a conserved sequence available for base pairing with pre-rRNA.
    Mol Cell Biol. 1987 Aug;7(8):2899-913 PMID: 2959855
  41. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein.
    J Cell Biol. 1988 Jul;107(1):17-31 PMID: 3292539
  42. RNA11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus.
    Mol Cell Biol. 1988 Jun;8(6):2379-93 PMID: 3043176
  43. Pre-mRNA splicing by complementation with purified human U1, U2, U4/U6 and U5 snRNPs.
    Nucleic Acids Res. 1988 Oct 25;16(20):9415-29 PMID: 3141901
  44. U2 small nuclear RNA is remarkably conserved between Schizosaccharomyces pombe and mammals.
    Mol Cell Biol. 1988 Dec;8(12):5575-80 PMID: 3244367
  45. U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
    EMBO J. 1989 Oct;8(10):3113-9 PMID: 2531075
  46. Higher order nuclear organization: three-dimensional distribution of small nuclear ribonucleoprotein particles.
    Proc Natl Acad Sci U S A. 1990 Jan;87(1):147-51 PMID: 2136950
  47. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus.
    Nature. 1990 Feb 1;343(6257):437-41 PMID: 2137203
  48. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
    J Cell Biol. 1963 Apr;17:208-12 PMID: 13986422
  49. SnRNAs, SnRNPs, and RNA processing.
    Annu Rev Biochem. 1982;51:617-54 PMID: 6180681
  50. Electron microscopic localization of silver staining NOR-proteins in rat liver nucleoli upon D-galactosamine block of transcription.
    Eur J Cell Biol. 1982 Oct;28(2):272-7 PMID: 6184230
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.7.3524-3534.1990
Published
1990-07-00
Pages
3524-34
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360787
Subset
IM
Grants
NIGMS NIH HHS · R01 GM042694 · United States
NCI NIH HHS · 5P30 CA45508-03 · United States
NIGMS NIH HHS · GM42694 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.7.3524-3534.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2