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PMID: 3536958 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins.

The Journal of cell biology ·Vol. 103 ·No. 6 Pt 1 ·1986-12-00 ·Pages 2103-12

Last RL, Woolford JL

Abstract

Temperature-sensitive mutations in the RNA2 through RNA11 genes of yeast prevent the processing of nuclear pre-mRNAs. We have raised antisera that detect the RNA2 and RNA3 proteins in immunoblots of extracts of yeast containing high copy number RNA2 and RNA3 plasmids. Subcellular fractionation of yeast cells that overproduce the RNA2 and RNA3 proteins has revealed that these proteins are enriched in nuclear fractions. Indirect immunofluorescence results have indicated that these proteins are localized in yeast nuclei. These localization results are consistent with the fact that these genes have a role in processing yeast pre-mRNA.

MeSH Terms
Cell Nucleus/metabolism,ultrastructure Fluorescent Antibody Technique Fungal Proteins/analysis,genetics Genes Genes, Fungal Immune Sera Mutation Nucleic Acid Precursors/genetics Plasmids RNA Precursors RNA Processing, Post-Transcriptional RNA, Messenger/genetics Saccharomyces cerevisiae/genetics
Chemicals
Fungal Proteins Immune Sera Nucleic Acid Precursors RNA Precursors RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Last R L
Woolford J L
References (45)
45 references, click to expand
  1. Macromolecule synthesis in temperature-sensitive mutants of yeast.
    J Bacteriol. 1967 May;93(5):1662-70 PMID: 5337848
  2. Yeast mRNA splicing in vitro.
    J Biol Chem. 1985 Nov 25;260(27):14780-92 PMID: 2997224
  3. The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA.
    Methods Cell Biol. 1975;12:335-51 PMID: 1105070
  4. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  5. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  6. Labeling of proteins with beta-galactosidase by gene fusion. Identification of a cytoplasmic membrane component of the Escherichia coli maltose transport system.
    J Biol Chem. 1980 Jan 10;255(1):168-74 PMID: 6765939
  7. Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6 PMID: 7001447
  8. Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae.
    Nucleic Acids Res. 1980 Mar 11;8(5):1043-59 PMID: 7003553
  9. The effect of temperature-sensitive RNA mutants on the transcription products from cloned ribosomal protein genes of yeast.
    Cell. 1981 Jun;24(3):679-86 PMID: 7018694
  10. The genes for fifteen ribosomal proteins of Saccharomyces cerevisiae.
    J Biol Chem. 1981 Oct 10;256(19):10176-83 PMID: 6268628
  11. Human growth hormone DNA sequence and mRNA structure: possible alternative splicing.
    Nucleic Acids Res. 1981 Aug 11;9(15):3719-30 PMID: 6269091
  12. Adenovirus gene expression: control at multiple steps of mRNA biogenesis.
    Cell. 1982 Jan;28(1):1-2 PMID: 7066982
  13. Isolation and characterization of a proteinaceous subnuclear fraction composed of nuclear matrix, peripheral lamina, and nuclear pore complexes from embryos of Drosophila melanogaster.
    J Cell Biol. 1982 Mar;92(3):674-86 PMID: 6177701
  14. A suppressor of temperature-sensitive rna mutations that affect mRNA metabolism in Saccharomyces cerevisiae.
    Mol Cell Biol. 1982 May;2(5):571-77 PMID: 7050675
  15. Open reading frame cloning: identification, cloning, and expression of open reading frame DNA.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6598-602 PMID: 6815653
  16. Molecular cloning and analysis of the CRY1 gene: a yeast ribosomal protein gene.
    Nucleic Acids Res. 1983 Jan 25;11(2):403-20 PMID: 6338478
  17. Polyadenylylated nuclear RNA contains branches.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):950-4 PMID: 6573664
  18. Open reading frame expression vectors: a general method for antigen production in Escherichia coli using protein fusions to beta-galactosidase.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4432-6 PMID: 6308625
  19. Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts.
    Cell. 1983 Nov;35(1):89-99 PMID: 6194902
  20. India ink staining of proteins on nitrocellulose paper.
    Anal Biochem. 1983 Aug;133(1):157-62 PMID: 6195938
  21. The rna2 mutation of yeast affects the processing of actin mRNA as well as ribosomal protein mRNAs.
    Mol Gen Genet. 1983;192(1-2):101-3 PMID: 6358792
  22. Three different fibronectin mRNAs arise by alternative splicing within the coding region.
    Cell. 1983 Dec;35(2 Pt 1):421-31 PMID: 6317187
  23. Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae.
    J Cell Biol. 1984 Mar;98(3):934-45 PMID: 6365931
  24. Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro.
    Cell. 1984 Apr;36(4):993-1005 PMID: 6323033
  25. Physical change in cytoplasmic messenger ribonucleoproteins in cells treated with inhibitors of mRNA transcription.
    Mol Cell Biol. 1984 Mar;4(3):415-23 PMID: 6717428
  26. Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate.
    Cell. 1984 Jun;37(2):415-27 PMID: 6722880
  27. The yeast MATa1 gene contains two introns.
    EMBO J. 1984 May;3(5):1061-5 PMID: 6329735
  28. Isolation and initial characterization of residual nuclear structures from yeast.
    Exp Cell Res. 1984 Aug;153(2):374-88 PMID: 6203771
  29. Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots.
    J Cell Biol. 1984 Jul;99(1 Pt 1):20-8 PMID: 6203917
  30. Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.
    Cell. 1984 Aug;38(1):317-31 PMID: 6088074
  31. Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors.
    Science. 1984 Aug 31;225(4665):898-903 PMID: 6206566
  32. Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):5951-5 PMID: 6091123
  33. Fast skeletal muscle myosin light chains 1 and 3 are produced from a single gene by a combined process of differential RNA transcription and splicing.
    J Biol Chem. 1984 Nov 10;259(21):13595-604 PMID: 6092382
  34. Isolation and characterization of the RNA2+, RNA4+, and RNA11+ genes of Saccharomyces cerevisiae.
    J Bacteriol. 1984 Dec;160(3):1093-100 PMID: 6094499
  35. Functions of purified E1A protein microinjected into mammalian cells.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6988-92 PMID: 6334304
  36. In vivo splicing products of the rabbit beta-globin pre-mRNA.
    Cell. 1984 Dec;39(3 Pt 2):589-602 PMID: 6096012
  37. In vivo characterization of yeast mRNA processing intermediates.
    Cell. 1984 Dec;39(3 Pt 2):603-10 PMID: 6096013
  38. Lariat structures are in vivo intermediates in yeast pre-mRNA splicing.
    Cell. 1984 Dec;39(3 Pt 2):611-21 PMID: 6096014
  39. Isolation and characterization of the RNA2, RNA3, and RNA11 genes of Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Nov;4(11):2396-405 PMID: 6083441
  40. Cloning of the RNA2 gene of Saccharomyces cerevisiae.
    EMBO J. 1984 Dec 1;3(12):2825-30 PMID: 6098448
  41. The "spliceosome": yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction.
    Science. 1985 May 24;228(4702):963-7 PMID: 3890181
  42. A multicomponent complex is involved in the splicing of messenger RNA precursors.
    Cell. 1985 Aug;42(1):345-53 PMID: 3160482
  43. Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences.
    Cell. 1985 Aug;42(1):355-67 PMID: 3160483
  44. Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro.
    Cell. 1985 Oct;42(3):725-36 PMID: 2996774
  45. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.103.6.2103
Published
1986-12-00
Pages
2103-12
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114587
Subset
IM
Grants
NCI NIH HHS · CA-01000 · United States
NIGMS NIH HHS · GM-08067 · United States
NIGMS NIH HHS · GM-28301 · United States
External Links
PubMed source DOI: 10.1083/jcb.103.6.2103 PMC Full Text View journal details
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