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PMID: 6094499 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of the RNA2+, RNA4+, and RNA11+ genes of Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 160 ·No. 3 ·1984-12-00 ·Pages 1093-100

Soltyk A, Tropak M, Friesen JD

Abstract

We used genetic complementation to isolate DNA fragments that encode the Saccharomyces cerevisiae genes RNA2+, RNA4+, and RNA11+ and to localize the genes on the cloned DNA fragments. RNA blot-hybridization analyses coupled with genetic analyses indicated the RNA2+ is coded by a 3.0-kilobase (kb) transcript, RNA4+ is coded by a 1.6-kb transcript, and RNA11+ is coded by a 1.3-kb or a 1.7-kb transcript or both; none of the cloned genes contains detectable introns. All three genes were transcribed into messages of very low abundance (approximately 20 times lower than a ribosomal protein message). DNA blot-hybridization revealed that all cloned genes are represented only once in the yeast chromosome. mRNA for RNA2+ and RNA4+ is produced in approximate proportion to gene dosage, whereas RNA11+ transcription appears to be not nearly so dependent on gene dosage. On a medium-copy plasmid (5 to 10 copies per cell), each cloned gene complemented mutations only in its own gene, indicating that each gene encodes a unique function. Genetic analysis by integrative transformation indicated that we cloned the RNA2+, RNA4+, and RNA11+ structural genes and not second-site suppressors.

MeSH Terms
Base Sequence Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes DNA, Fungal/isolation & purification Genes, Fungal Nucleic Acid Hybridization Plasmids RNA, Fungal/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
DNA, Fungal RNA, Fungal DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Soltyk A
Tropak M
Friesen J D
References (52)
52 references, click to expand
  1. Are snRNPs involved in splicing?
    Nature. 1980 Jan 10;283(5743):220-4 PMID: 7350545
  2. Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease.
    Cell. 1983 Feb;32(2):525-36 PMID: 6186398
  3. Positive regulation in the general amino acid control of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5374-8 PMID: 6351059
  4. Detection and utilization of poly(A) sequences in messenger RNA.
    Methods Cell Biol. 1973;7:53-65 PMID: 4798608
  5. The yeast MATa1 gene contains two introns.
    EMBO J. 1984 May;3(5):1061-5 PMID: 6329735
  6. The presence of a defective LEU2 gene on 2 mu DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number.
    J Bacteriol. 1983 Nov;156(2):625-35 PMID: 6313610
  7. Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4403-7 PMID: 6308621
  8. Ribosomal RNA transcription in a mutant of Saccharomyces cerevisiae defective in ribosomal protein synthesis.
    Mol Gen Genet. 1978 May 3;161(2):221-3 PMID: 353511
  9. The role of cytoplasmic membranes in controlling the transport of nuclear messenger RNA and initiation of protein synthesis.
    Proc Natl Acad Sci U S A. 1974 Jul;71(7):2658-62 PMID: 4605329
  10. The genes for fifteen ribosomal proteins of Saccharomyces cerevisiae.
    J Biol Chem. 1981 Oct 10;256(19):10176-83 PMID: 6268628
  11. The effect of temperature-sensitive RNA mutants on the transcription products from cloned ribosomal protein genes of yeast.
    Cell. 1981 Jun;24(3):679-86 PMID: 7018694
  12. Coordinate regulation of the synthesis of eukaryotic ribosomal proteins.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1547-51 PMID: 775493
  13. Isolation and initial characterization of residual nuclear structures from yeast.
    Exp Cell Res. 1984 Aug;153(2):374-88 PMID: 6203771
  14. Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6 PMID: 7001447
  15. Regulation of a set of abundant mRNA sequences.
    Cell. 1977 Mar;10(3):463-7 PMID: 321130
  16. Genetic map of Saccharomyces cerevisiae.
    Microbiol Rev. 1980 Dec;44(4):519-71 PMID: 7010111
  17. Identification and characterization of the packaging proteins of core 40S hnRNP particles.
    Cell. 1977 May;11(1):127-38 PMID: 872217
  18. Temperature-sensitive mutants of yeast exhibiting a rapid inhibition of protein synthesis.
    J Bacteriol. 1968 Nov;96(5):1664-71 PMID: 5726307
  19. The structure of the gene coding for the phosphorylated ribosomal protein S10 in yeast.
    Nucleic Acids Res. 1982 Oct 11;10(19):5869-78 PMID: 6292856
  20. Mapping of trichodermin resistance in Saccharomyces cerevisiae: a genetic locus for a component of the 60S ribsomal subunit.
    Genetics. 1976 Aug;83(4):667-73 PMID: 786781
  21. Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes.
    Genetics. 1973 May;74(1):33-54 PMID: 17248609
  22. The rna2 mutation of yeast affects the processing of actin mRNA as well as ribosomal protein mRNAs.
    Mol Gen Genet. 1983;192(1-2):101-3 PMID: 6358792
  23. A yeast mutant which accumulates precursor tRNAs.
    Cell. 1978 Jun;14(2):211-9 PMID: 352536
  24. Functional organization in the nucleus.
    Int Rev Cytol. 1982;79:165-214 PMID: 6185451
  25. Identification of ten genes that control ribosome formation in yeast.
    Mol Gen Genet. 1970;109(1):42-56 PMID: 5488085
  26. Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron.
    Cell. 1980 Nov;22(2 Pt 2):333-48 PMID: 7004642
  27. Further evidence that the rna2 mutation of Saccharomyces cerevisiae affects mRNA processing.
    Mol Cell Biol. 1982 Oct;2(10):1205-11 PMID: 6757717
  28. Mechanism of action of a yeast RNA ligase in tRNA splicing.
    Cell. 1983 Feb;32(2):537-46 PMID: 6297798
  29. Temperature-sensitive yeast mutant defective in ribonucleic acid production.
    J Bacteriol. 1969 Sep;99(3):807-14 PMID: 5370278
  30. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  31. Chimeric plasmids for cloning of deoxyribonucleic acid sequences in Saccharomyces cerevisiae.
    J Bacteriol. 1979 Oct;140(1):73-82 PMID: 387728
  32. Yeast DNA plasmids.
    Annu Rev Microbiol. 1983;37:253-76 PMID: 6357054
  33. Yeast contains small nuclear RNAs encoded by single copy genes.
    Cell. 1983 Dec;35(3 Pt 2):743-51 PMID: 6197183
  34. Nucleotide sequence of the tcml gene (ribosomal protein L3) of Saccharomyces cerevisiae.
    J Bacteriol. 1983 Jul;155(1):8-14 PMID: 6305925
  35. Molecular cloning and analysis of the CRY1 gene: a yeast ribosomal protein gene.
    Nucleic Acids Res. 1983 Jan 25;11(2):403-20 PMID: 6338478
  36. Rapid DNA isolations for enzymatic and hybridization analysis.
    Methods Enzymol. 1980;65(1):404-11 PMID: 6246361
  37. Purification and properties of poliovirus double-stranded ribonucleic acid.
    J Mol Biol. 1966 Jul;18(3):421-8 PMID: 4290740
  38. A suppressor of temperature-sensitive rna mutations that affect mRNA metabolism in Saccharomyces cerevisiae.
    Mol Cell Biol. 1982 May;2(5):571-77 PMID: 7050675
  39. Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.
    Cell. 1983 Apr;32(4):1279-86 PMID: 6301690
  40. Splicing and the formation of stable RNA.
    Cell. 1979 Dec;18(4):1299-302 PMID: 229971
  41. Splicing of yeast tRNA precursors: a two-stage reaction.
    Cell. 1979 Sep;18(1):27-35 PMID: 389431
  42. Nuclear RNA-protein interactions and messenger RNA processing.
    J Cell Biol. 1983 Nov;97(5 Pt 1):1321-6 PMID: 6355116
  43. Heterogeneity and territorial organization of the nuclear matrix and related structures.
    Int Rev Cytol. 1983;83:135-82 PMID: 6358101
  44. Transcription and processing of intervening sequences in yeast tRNA genes.
    Cell. 1978 Jun;14(2):221-36 PMID: 352537
  45. Nucleotide sequence deletions within the coding region for small-t antigen of simian virus 40.
    J Virol. 1979 Jun;30(3):674-82 PMID: 225536
  46. Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2546-50 PMID: 6994099
  47. The synthesis of eucaryotic ribosomal proteins in vitro.
    Cell. 1977 May;11(1):201-12 PMID: 326416
  48. Yeast transformation: a model system for the study of recombination.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354-8 PMID: 6273866
  49. The new yeast genetics.
    Nature. 1983 Sep 29-Oct 5;305(5933):391-7 PMID: 6353245
  50. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.
    Gene. 1979 Dec;8(1):17-24 PMID: 395030
  51. Characterization of the non-histone nuclear proteins associated with rapidly labeled heterogeneous nuclear RNA.
    J Biol Chem. 1977 Oct 25;252(20):7307-22 PMID: 903364
  52. Two-dimensinal gel electrophoresis of rat liver nuclear washes, nuclear matrix, and hnRNA proteins.
    J Cell Biol. 1980 Jul;86(1):135-55 PMID: 7419573
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.3.1093-1100.1984
Published
1984-12-00
Pages
1093-100
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215824
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.160.3.1093-1100.1984 PMC Full Text View journal details
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