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PMID: 6994099 Published · ppublish English Journal Article

Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 5 ·1980-05-00 ·Pages 2546-50

Gallwitz D, Sures I

Abstract

The complete nucleotide sequence of the actin gene from Saccharomyces cerevisiae has been determined. The coding region is interrupted by a 304-base-pair intervening sequence that is located within the triplet coding for amino acid 4. DNA sequences of the intron-exon junctions are similar to those found in higher eukaryotes and can be aligned such that the intron starts with the dinucleotide 5'-G-T-3' and ends with 5'-A-G-3'. Regions fo homology within the sequences upstream from the initiation codon and those following the termination codon have been detected between the yeast iso-1-cytochrome c gene and the actin gene. As deduced from the nucleotide sequence, yeast actin has 374 amino acid residues. Its primary structure, especially the NH2-terminal third of the protein, is highly conserved during evolution.

MeSH Terms
Actins/genetics Amino Acids/analysis Base Sequence Codon DNA, Recombinant Genes Nucleic Acid Precursors/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics
Chemicals
Actins Amino Acids Codon DNA, Recombinant Nucleic Acid Precursors RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gallwitz D
Sures I
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.5.2546
Published
1980-05-00
Pages
2546-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349438
Subset
IM
Databases
GENBANK
J01310, L00026
External Links
PubMed source DOI: 10.1073/pnas.77.5.2546 PMC Full Text View journal details
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