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PMID: 6269091 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human growth hormone DNA sequence and mRNA structure: possible alternative splicing.

Nucleic acids research ·Vol. 9 ·No. 15 ·1981-08-11 ·Pages 3719-30

DeNoto FM, Moore DD, Goodman HM

Abstract

We have determined the complete sequence of the human growth hormone (hGH) gene and the position of the mature 5' end of the hGH mRNA within the sequence. Comparison of this sequence with that of a cloned hGH cDNA shows that the gene is interrupted by four intervening sequences. S1 mapping shows that one of these intervening sequences has two different 3' splice sites. These alternate splicing pathways generate hGH peptides of different sizes which are found in normal pituitaries. Comparison of sequences near the 5' end of the hGH mRNA with a similar region of the alpha subunit of the human glycoprotein hormones reveals an unexpected region of homology between these otherwise unrelated peptide hormones.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant Genes Growth Hormone/genetics Humans Pituitary Gland/analysis RNA, Messenger/genetics
Chemicals
DNA, Recombinant RNA, Messenger Growth Hormone DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
DeNoto F M
Moore D D
Goodman H M
References (30)
30 references, click to expand
  1. Sequences of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene reduplication.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):866-70 PMID: 5279528
  2. The regulation of growth by endocrines.
    Annu Rev Physiol. 1975;37:211-44 PMID: 804843
  3. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  4. Construction and analysis of recombinant DNA for human chorionic somatomammotropin.
    Nature. 1977 Dec 8;270(5637):494-9 PMID: 593368
  5. Why genes in pieces?
    Nature. 1978 Feb 9;271(5645):501 PMID: 622185
  6. Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1485-9 PMID: 418414
  7. Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4853-7 PMID: 283395
  8. Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segments.
    Nature. 1979 Feb 22;277(5698):627-33 PMID: 106304
  9. Human growth hormone: additional members of the complex.
    Endocrinology. 1979 May;104(5):1256-65 PMID: 35338
  10. The ovalbumin gene: cloning and molecular organization of the entire natural gene.
    Proc Natl Acad Sci U S A. 1979 May;76(5):2253-7 PMID: 287066
  11. BKV splice sequences based on analysis of preferred donor and acceptor sites.
    Nucleic Acids Res. 1979 Jul 25;6(10):3387-98 PMID: 225729
  12. Molecular cloning and nucleotide sequence of the human growth hormone structural gene.
    Nucleic Acids Res. 1979 Sep 25;7(2):305-20 PMID: 386281
  13. Isolation and characterization of a cloned rat insulin gene.
    Cell. 1979 Oct;18(2):533-43 PMID: 498283
  14. The structure and evolution of the two nonallelic rat preproinsulin genes.
    Cell. 1979 Oct;18(2):545-58 PMID: 498284
  15. Structure of genes for human growth hormone and chorionic somatomammotropin.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4294-8 PMID: 291965
  16. Are snRNPs involved in splicing?
    Nature. 1980 Jan 10;283(5743):220-4 PMID: 7350545
  17. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  18. The complete sequence of a chromosomal mouse alpha--globin gene reveals elements conserved throughout vertebrate evolution.
    Cell. 1979 Nov;18(3):875-82 PMID: 519760
  19. Sequence of the human insulin gene.
    Nature. 1980 Mar 6;284(5751):26-32 PMID: 6243748
  20. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  21. The 20,000-dalton variant of human growth hormone: location of the amino acid deletions.
    Biochem Biophys Res Commun. 1980 Jan 29;92(2):511-6 PMID: 7356479
  22. Structure and genomic organization of the mouse dihydrofolate reductase gene.
    Cell. 1980 Feb;19(2):355-64 PMID: 6244105
  23. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  24. Characterization of globin domains: heme binding to the central exon product.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1384-8 PMID: 6929492
  25. A mechanism for RNA splicing.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1877-9 PMID: 6246511
  26. Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3' ends.
    Cell. 1980 Jun;20(2):293-301 PMID: 6771018
  27. Two mRNAs can be produced from a single immunoglobulin mu gene by alternative RNA processing pathways.
    Cell. 1980 Jun;20(2):313-9 PMID: 6771020
  28. Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2546-50 PMID: 6994099
  29. Organization and sequence studies of the 17-piece chicken conalbumin gene.
    Nature. 1979 Dec 6;282(5739):567-74 PMID: 551292
  30. Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6 PMID: 7001447
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.15.3719
Published
1981-08-11
Pages
3719-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327387
Subset
IM
Grants
NIADDK NIH HHS · AM19997 · United States
NCI NIH HHS · CA14026 · United States
Databases
GENBANK
J00148, K00612
External Links
PubMed source DOI: 10.1093/nar/9.15.3719 PMC Full Text View journal details
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