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PMID: 291965 Published · ppublish English Journal Article

Structure of genes for human growth hormone and chorionic somatomammotropin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 9 ·1979-09-00 ·Pages 4294-8

Fiddes JC, Seeburg PH, DeNoto FM, Hallewell RA, Baxter JD, Goodman HM

Abstract

A 2.6-kilobase (kb) EcoRI restriction endonuclease fragment containing human growth hormone (hGH; somatotropin) gene sequences and a 2.8-kb EcoRI fragment containing human chorionic somatomammotropin (hCS; choriomammotropin) gene sequences have been identified by hybridization to cloned cDNA. Human DNA was cleaved with EcoRI and fractionated by preparative agarose gel electrophoresis; DNA in the size range 2--3 kb was ligated to lambda gt WES.lambda B DNA and viable recombinant bacteriophage were recovered by in vitro packaging. After infection of Escherichia coli and screening of phage plaques, single isolates of hGH and hCS gene sequences were obtained. Restriction endonuclease mapping showed that the hGH gene contains three intervening sequences interrupting the coding sequence. Partial DNA sequence analysis of the hGH gene, obtained by the chain termination method, confirmed the location of the intervening sequences and the identity of the fragment.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Recombinant Genes Growth Hormone/genetics Humans Nucleic Acid Precursors/genetics Placental Lactogen/genetics RNA, Messenger/genetics Transcription, Genetic
Chemicals
DNA, Recombinant Nucleic Acid Precursors RNA, Messenger Growth Hormone Placental Lactogen
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fiddes J C
Seeburg P H
DeNoto F M
Hallewell R A
Baxter J D
Goodman H M
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.9.4294
Published
1979-09-00
Pages
4294-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411560
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.9.4294 PMC Full Text View journal details
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