The use of exonuclease III for preparing single stranded DNA for use as a template in the chain terminator sequencing method.

Nucleic acids research ·Vol. 6 ·No. 3 ·1979-03-00 ·Pages 831-48

Smith AJ

Abstract

Exonuclease III, which degrades DNA 3' leads to 5' and is specific for duplex DNA, can be used to prepare single stranded DNA from linear duplexes. This is shown to be suitable as a template for use with the chain terminator DNA sequencing method of Sanger et al. [1]. Strategies are discussed for preparing single stranded DNA templates by this method and, in particular, its application to the sequence analysis of DNA cloned in plasmid vectors.

MeSH Terms

Base Sequence DNA, Single-Stranded/biosynthesis Deoxyribonucleases Exodeoxyribonucleases Exonucleases Oligodeoxyribonucleotides/analysis Templates, Genetic

Chemicals

DNA, Single-Stranded Oligodeoxyribonucleotides Deoxyribonucleases Exodeoxyribonucleases Exonucleases exodeoxyribonuclease III

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Smith A J

References (9)

9 references, click to expand

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DNA sequencing with chain-terminating inhibitors.
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A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
Is there a correction mechanism in the 5S multigene system?
Nature. 1978 Oct 12;275(5680):556-8 PMID: 567752
The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. I. The AT-rich spacer.
Cell. 1978 Apr;13(4):701-16 PMID: 566163
The use of thin acrylamide gels for DNA sequencing.
FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA.
J Mol Biol. 1974 Aug 15;87(3):377-407 PMID: 4374546
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.3.831

Published

1979-03-00

Pages

831-48

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327737

Subset

External Links

PubMed source DOI: 10.1093/nar/6.3.831 PMC Full Text View journal details

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The use of exonuclease III for preparing single stranded DNA for use as a template in the chain terminator sequencing method.

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