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PMID: 273235 Published · ppublish English Journal Article

Intervening sequence of DNA identified in the structural portion of a mouse beta-globin gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 2 ·1978-02-00 ·Pages 725-9

Tilghman SM, Tiemeier DC, Seidman JG, Peterlin BM, Sullivan M, Maizel JV, Leder P

Abstract

The unusual electron microscopic appearance of a hybrid formed between 9S mouse beta-globin mRNA and its corresponding cloned gene segment is caused by at least one, and possibly two, intervening sequences of DNA that interrupt the mouse beta-globin gene. Such an interpretation is consistent with a paradoxical restriction site pattern previously noted in this gene and with the nucleotide sequence of that portion of the gene that spans both structural and intervening sequences. The large intervening sequence, approximately 550 base pairs in length, occurs in the structural globin sequence and immediately follows the beta-globin codon corresponding to amino acid 104. A smaller, putative intervening sequence is located close to the 5' end of the beta-globin-coding sequence but may reside beyond its initiation codon. The beta-globin gene thus appears to be encoded in two, and possibly three, discontinuous segments.

MeSH Terms
Animals Base Sequence Chromosome Mapping DNA Restriction Enzymes Genes Globins/biosynthesis Mice Microscopy, Electron
Chemicals
Globins DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Tilghman S M
Tiemeier D C
Seidman J G
Peterlin B M
Sullivan M
Maizel J V
Leder P
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.2.725
Published
1978-02-00
Pages
725-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411329
Subset
IM
Databases
GENBANK
M10688
External Links
PubMed source DOI: 10.1073/pnas.75.2.725 PMC Full Text View journal details
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