Packaging recombinant DNA molecules into bacteriophage particles in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 8 ·1977-08-00 ·Pages 3259-63

Hohn B, Murray K

Abstract

Recombinant phage genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro. The process is efficient and nonselective and offers containment in initial stages of handling recombinant DNA. Ligase [poly(deoxyribonucleotide):poly-(deoxyribonucleotide) ligase (AMP-forming), EC 6.5.1.1] reaction products can recombine with endogenous phage DNA during packaging, but UV-irradiation eliminates the biological activity of the endogenous DNA.

MeSH Terms

Coliphages/metabolism,radiation effects DNA Ligases/metabolism DNA Restriction Enzymes DNA, Bacterial/metabolism DNA, Recombinant/metabolism,radiation effects DNA, Viral/metabolism Escherichia coli/metabolism,radiation effects Genotype Mutation Species Specificity Transfection Ultraviolet Rays

Chemicals

DNA, Bacterial DNA, Recombinant DNA, Viral DNA Restriction Enzymes DNA Ligases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hohn B

Murray K

References (28)

28 references, click to expand

Capsid transformation during packaging of bacteriophage lambdaDNA.
Philos Trans R Soc Lond B Biol Sci. 1976 Nov 30;276(943):51-61 PMID: 13435
In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1.
Gene. 1977 May;1(3-4):255-80 PMID: 338419
Functional expression of cloned yeast DNA in Escherichia coli.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):487-91 PMID: 322128
Lambdoid phages that simplify the recovery of in vitro recombinants.
Mol Gen Genet. 1977 Jan 7;150(1):53-61 PMID: 319344
Replication and transcription of eukaryotic DNA in Escherichia coli.
Proc Natl Acad Sci U S A. 1974 May;71(5):1743-7 PMID: 4600264
Excision of prophage lambda in a cell-free system.
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2188-92 PMID: 1094457
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids.
Cell. 1975 Oct;6(2):231-44 PMID: 1102112
In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase.
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3416-20 PMID: 1103146
Restriction endonucleases in the analysis and restructuring of dna molecules.
Annu Rev Biochem. 1975;44:273-93 PMID: 166604
Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments.
Nature. 1974 Oct 11;251(5475):476-81 PMID: 4608939
Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli.
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1030-4 PMID: 4598290
Activity of empty, headlike particles for packaging of DNA of bacteriophage lambda in vitro.
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2372-6 PMID: 4601587
In vitro genetic recombination of bacteriophage lambda.
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2496-9 PMID: 4526306
Head assembly steps controlled by genes F and W in bacteriophage lambda.
J Mol Biol. 1972 Mar 14;64(3):551-63 PMID: 5022189
Calcium-dependent bacteriophage DNA infection.
J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage.
Proc Natl Acad Sci U S A. 1967 Apr;57(4):1021-8 PMID: 5340583
The construction in vitro of transducing derivatives of phage lambda.
Mol Gen Genet. 1976 Jul 23;146(2):199-207 PMID: 785220
Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli.
Nature. 1975 Jun 12;255(5509):533-8 PMID: 1095932
Tandem genetic duplications in phage lambda. III. The frequency of duplication mutants in two derivatives of phage lambda is independent of known recombination systems.
J Mol Biol. 1975 Jan 15;91(2):133-46 PMID: 1102698
Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.
J Mol Biol. 1975 Nov 5;98(3):551-64 PMID: 1104875
Petit lambda, a family of particles from coliphage lambda infected cells.
J Mol Biol. 1975 Oct 15;98(1):107-20 PMID: 1104865
Functional genetic expression of eukaryotic DNA in Escherichia coli.
Proc Natl Acad Sci U S A. 1976 May;73(5):1471-5 PMID: 775490
Dependence of transfection efficiency of calcium treated Escherichia coli cells on bacterial genotype and form of Lambda DNA.
Mol Gen Genet. 1976 Feb 27;144(1):83-6 PMID: 772416
Reduction of possible hazards in the preparation of recombinant plasmid DNA.
Nature. 1976 Jun 3;261(5559):428-9 PMID: 778626
Integrative recombination of bacteriophage lambda DNA in vitro.
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1072-6 PMID: 1055366
DNA as substrate for packaging into bacteriophage lambda, in vitro.
J Mol Biol. 1975 Oct 15;98(1):93-106 PMID: 1195386

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.8.3259

Published

1977-08-00

Pages

3259-63

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC431522

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.8.3259 PMC Full Text View journal details

Analysis Services

Packaging recombinant DNA molecules into bacteriophage particles in vitro.

Contact

WeChat Official Account