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PMID: 3244367 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

U2 small nuclear RNA is remarkably conserved between Schizosaccharomyces pombe and mammals.

Molecular and cellular biology ·Vol. 8 ·No. 12 ·1988-12-00 ·Pages 5575-80

Brennwald P, Porter G, Wise JA

Abstract

We report the molecular cloning and sequencing of the most abundant trimethylguanosine-capped small nuclear RNA from the fission yeast Schizosaccharomyces pombe, a highly conserved homolog of mammalian U2 small nuclear RNA. This RNA is 186 nucleotides in length, just 2 nucleotides shorter than its human counterpart; this is in contrast to Saccharomyces cerevisiae U2, which is 1,175 nucleotides long. Moreover, the secondary structure of Schizosaccharomyces pombe U2 is virtually identical to that of mammalian U2, including the 3' half of the RNA, which shows limited primary sequence identity. Northern (RNA) blot analysis revealed that the size of this RNA is conserved not only in fission yeasts but in many organisms, including other ascomycetes.

MeSH Terms
Animals Base Sequence Genes, Fungal Humans Molecular Sequence Data Nucleic Acid Conformation Plants/genetics RNA, Small Nuclear/genetics Restriction Mapping Saccharomycetales/genetics Schizosaccharomyces/genetics Sequence Homology, Nucleic Acid
Chemicals
RNA, Small Nuclear
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brennwald P
Department of Biochemistry, University of Illinois, Urbana-Champaign 61801.
Porter G
Wise J A
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34 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.12.5575-5580.1988
Published
1988-12-00
Pages
5575-80
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365665
Subset
IM
Grants
NIGMS NIH HHS · GM 38070 · United States
Databases
GENBANK
M23361
External Links
PubMed source DOI: 10.1128/mcb.8.12.5575-5580.1988 PMC Full Text View journal details
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