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PMID: 6181942 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The morphological relationship in electron microscopy between NOR-silver proteins and intranucleolar chromatin.

Chromosoma ·Vol. 85 ·No. 4 ·1982-00-00 ·Pages 461-73

Hernandez-Verdun D, Derenzini M, Bouteille M

Abstract

The relative distribution of NOR proteins and chromatin fibers in the nucleoli was visualized in human cell line. The chromatin was revealed by a Feulgen-like procedure using osmium-ammine as DNA tracer. This selective staining was combined with NOR-silver staining. We provide morphological evidence for constant overlapping of the silver deposit sites with dispersed intranucleolar chromatin fibers. Silver stained proteins were sometimes observed in contact with the chromatin fibers, suggesting that at least some of the Ag-NOR proteins might be closely connected with the dispersed nucleolar DNA.

MeSH Terms
Cell Line Cell Nucleolus/ultrastructure Chromatin/ultrastructure Humans Microscopy, Electron/methods Nuclear Proteins Nucleophosmin Nucleoproteins/analysis Ribonucleoproteins/analysis Silver Proteins Staining and Labeling
Chemicals
Chromatin Nuclear Proteins Nucleoproteins Ribonucleoproteins Silver Proteins Nucleophosmin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hernandez-Verdun D
Derenzini M
Bouteille M
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42 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00327343
Published
1982-00-00
Pages
461-73
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327343 View journal details
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