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PMID: 88405 Published · ppublish English Journal Article

Correlated inhibition of ribosomal RNA synthesis and silver staining by actinomycin D.

Human genetics ·Vol. 47 ·No. 3 ·1979-04-05 ·Pages 329-33

Hofgärtner FJ, Krone W, Jain K

Abstract

Preferential inhibition of the synthesis of ribosomal RNA by low doses of actinomycin D was used to investigate the quantitative relationship between the intensity of silver staining of nucleoli and the rate of RNA-synthesis. The two parameters were found to be strongly correlated in human diploid fibroblasts.

MeSH Terms
Cell Nucleolus/drug effects Dactinomycin/pharmacology Fibroblasts/drug effects,ultrastructure Humans RNA, Ribosomal/antagonists & inhibitors,biosynthesis Silver Proteins Staining and Labeling
Chemicals
RNA, Ribosomal Silver Proteins Dactinomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hofgärtner F J
Krone W
Jain K
References (12)
12 references, click to expand
  1. THE EFFECT OF ACTINOMYCIN D ON THE FINE STRUCTURE OF THE NUCLEOLUS.
    J Ultrastruct Res. 1964 Apr;10:224-43 PMID: 14166291
  2. The nucleolar structure.
    Int Rev Cytol. 1976;44:1-28 PMID: 770368
  3. Evidence for postmeiotic expression of ribosomal RNA genes during male gametogenesis.
    Hum Genet. 1977 Oct 14;38(3):279-84 PMID: 914276
  4. Early alterations in amino acid pools and protein synthesis of diploid fibroblasts stimulated to synthesize DNA by addition of serum.
    J Cell Physiol. 1969 Oct;74(2):191-202 PMID: 5358255
  5. Onset of nucleolus organizer activity in early mouse embryogenesis and evidence for its regulation.
    Exp Cell Res. 1978 Jul;114(2):263-8 PMID: 567122
  6. Structure and synthesis of ribosomal RNA.
    Annu Rev Biochem. 1970;39:183-226 PMID: 4920820
  7. The nature of the Ag-staining of nucleolus organizer regions. Electron- and light-microscopic studies on human cells in interphase, mitosis, and meiosis.
    Cytogenet Cell Genet. 1978;20(1-6):24-39 PMID: 77208
  8. Activation of mouse ribosomal RNA genes at the 2-cell stage.
    Hum Genet. 1977 Aug 31;38(1):57-63 PMID: 903155
  9. NUCLEOLAR "CAPS" PRODUCED BY ACTINOMYCIN D.
    Cancer Res. 1964 Aug;24:1269-77 PMID: 14216161
  10. Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4531-5 PMID: 1070003
  11. SEPARATION AND SOME PROPERTIES OF DISTINCT CLASSES OF MEWLY-FORMED RIBONUCLEIC ACID FROM ANIMAL CELLS.
    Biochim Biophys Acta. 1964 Dec 16;91:678-80 PMID: 14262465
  12. Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells.
    Exp Cell Res. 1976 Sep;101(2):235-43 PMID: 61125
Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00321025
Published
1979-04-05
Pages
329-33
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00321025 View journal details
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