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PMID: 2406561 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 10 ·No. 3 ·1990-03-00 ·Pages 1145-52

Li HD, Zagorski J, Fournier MJ

Abstract

Repression of an essential nucleolar small nuclear RNA (snRNA) gene of Saccharomyces cerevisiae was shown to result in impaired production of 18S rRNA. The effect, observed for an snRNA species of 128 nucleotides (snR128), was evident within one generation after the onset of SNR128 gene repression and correlated well with depletion of the snRNA. The steady-state mass ratio of 18S RNA to 25S RNA decreased eightfold over the course of the analysis. Results from pulse-chase assays revealed the basis of the imbalance to be underaccumulation of 18S RNA and its 20S precursor. This effect appears to result from impairment of processing of the 35S rRNA transcript at sites that define the 20S species coupled with rapid turnover of unstable intermediates. Possible bases for the effects observed are discussed. A common U14 designation is proposed for the structurally related yeast snRNA and 4.5S hybRNAs from amphibians and mammals.

MeSH Terms
Base Sequence Cloning, Molecular Genetic Vectors Kinetics Molecular Sequence Data RNA Processing, Post-Transcriptional RNA, Fungal/metabolism RNA, Ribosomal/metabolism RNA, Ribosomal, 18S/metabolism RNA, Small Nuclear/physiology Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid
Chemicals
RNA, Fungal RNA, Ribosomal RNA, Ribosomal, 18S RNA, Small Nuclear
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Li H D
Department of Biochemistry, Lederle Graduate Research Center, University of Massachusetts, Amherst 01003.
Zagorski J
Fournier M J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.3.1145-1152.1990
Published
1990-03-00
Pages
1145-52
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360983
Subset
IM
Grants
NIGMS NIH HHS · GM19351 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.3.1145-1152.1990 PMC Full Text View journal details
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