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PMID: 2955216 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Both conserved signals on mammalian histone pre-mRNAs associate with small nuclear ribonucleoproteins during 3' end formation in vitro.

Molecular and cellular biology ·Vol. 7 ·No. 5 ·1987-05-00 ·Pages 1663-72

Mowry KL, Steitz JA

Abstract

Pre-mRNA substrates containing sequences from human and mouse histone genes are accurately processed in a HeLa cell nuclear extract to generate mature 3' termini. When in vitro processing reactions containing either human histone H3 or mouse histone H3 transcripts are treated with RNase T1 and probed with antibodies specific for the Sm protein determinants or for the trimethylguanosine cap structure unique to the U RNAs present in small nuclear ribonucleoproteins, RNA fragments that encompass the site of 3' end formation on the pre-mRNA transcript are selectively recovered. Several different interactions are detected: at time zero, the protected region contains the upstream conserved hairpin loop structure; at later times during the reaction, protection extends beyond the site of 3' end formation to include the downstream conserved sequence element and the 5' cap of the transcript is bound as well. Possible interactions between Sm small nuclear ribonucleoproteins and these conserved sequence elements in histone pre-mRNAs are discussed.

MeSH Terms
Autoantigens/immunology Binding Sites Histones/genetics Immunologic Techniques RNA Caps/immunology RNA Processing, Post-Transcriptional RNA, Messenger/genetics Ribonucleoproteins/physiology Ribonucleoproteins, Small Nuclear Time Factors snRNP Core Proteins
Chemicals
Autoantigens Histones RNA Caps RNA, Messenger Ribonucleoproteins Ribonucleoproteins, Small Nuclear snRNP Core Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mowry K L
Steitz J A
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44 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.5.1663-1672.1987
Published
1987-05-00
Pages
1663-72
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365266
Subset
IM
Grants
NCRR NIH HHS · 1 U41 RR-01685-03 · United States
NIGMS NIH HHS · GM 26154 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.5.1663-1672.1987 PMC Full Text View journal details
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