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PMID: 3015597 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Generation of histone mRNA 3' ends by endonucleolytic cleavage of the pre-mRNA in a snRNP-dependent in vitro reaction.

The EMBO journal ·Vol. 5 ·No. 6 ·1986-06-00 ·Pages 1319-26

Gick O, Krämer A, Keller W, Birnstiel ML

Abstract

Incubation of SP6 generated mouse histone H4 mRNA precursors in nuclear extracts of HeLa cells yields processed mRNA species which end on the 3' adenosine of the conserved terminal ACCA sequence not unlike ten different histone mRNAs isolated from sea urchin embryos which end either on the 3' C or A. In the presence of 20 mM EDTA, the cleaved off 3' spacer portions of the RNA transcripts are readily detected, hence the 3' ends of histone mRNAs arise as a consequence of endonucleolytic cleavage(s) of the precursor RNA. The in vitro cleavage reaction is specifically inhibited by human antisera of the Sm-serotype, but not by control sera and can be rescued by the addition of a preparation of partially purified small nuclear RNPs to the antibody-depleted extract. Interestingly, the snRNP preparation is sufficient to elicit 3' processing of pre-mRNA in the absence of added nuclear extract.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes Histones/genetics Mice Nucleic Acid Precursors/genetics RNA Precursors RNA, Messenger/genetics Ribonucleoproteins/genetics Ribonucleoproteins, Small Nuclear Sea Urchins Sequence Homology, Nucleic Acid Species Specificity Transcription, Genetic
Chemicals
Histones Nucleic Acid Precursors RNA Precursors RNA, Messenger Ribonucleoproteins Ribonucleoproteins, Small Nuclear DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gick O
Krämer A
Keller W
Birnstiel M L
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36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-06-00
Pages
1319-26
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166943
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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