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PMID: 2121740 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs.

The Journal of cell biology ·Vol. 111 ·No. 5 Pt 1 ·1990-11-00 ·Pages 1741-51

Clark MW, Yip ML, Campbell J, Abelson J

Abstract

SSB-1, the yeast single-strand RNA-binding protein, is demonstrated to be a yeast nucleolar-specific, silver-binding protein. In double-label immunofluorescence microscopy experiments antibodies to two other nucleolar proteins, RNA Pol I 190-kD and fibrillarin, were used to reveal the site of rRNA transcription; i.e., the fibrillar region of the nucleolus. SSB-1 colocalized with fibrillarin in a double-label immunofluorescence mapping experiment to the yeast nucleolus. SSB-1 is located, though, over a wider region of the nucleolus than the transcription site marker. Immunoprecipitations of yeast cell extracts with the SSB-1 antibody reveal that in 150 mM NaCl SSB-1 is bound to two small nuclear RNAs (snRNAs). These yeast snRNAs are snR10 and snR11, with snR10 being predominant. Since snR10 has been implicated in pre-rRNA processing, the association of SSB-1 and snR10 into a nucleolar snRNP particle indicates SSB-1 involvement in rRNA processing as well. Also, another yeast protein, SSB-36-kD, isolated by single-strand DNA chromatography, is shown to bind silver under the conditions used for nucleolar-specific staining. It is, most likely, another yeast nucleolar protein.

MeSH Terms
Antibodies Autoantibodies Autoantigens/analysis Carrier Proteins/analysis Chromosomal Proteins, Non-Histone/immunology DNA-Binding Proteins/analysis Fluorescent Antibody Technique Fungal Proteins/analysis Humans Microscopy, Fluorescence Molecular Weight Nucleolus Organizer Region/chemistry Precipitin Tests RNA Processing, Post-Transcriptional RNA, Fungal/metabolism RNA, Ribosomal/metabolism RNA, Small Nuclear/metabolism RNA-Binding Proteins Ribonucleoproteins Ribosomes/metabolism Saccharomyces cerevisiae/analysis,genetics Silver/metabolism Sodium Chloride
Chemicals
Antibodies Autoantibodies Autoantigens Carrier Proteins Chromosomal Proteins, Non-Histone DNA-Binding Proteins Fungal Proteins RNA, Fungal RNA, Ribosomal RNA, Small Nuclear RNA-Binding Proteins Ribonucleoproteins SS-B antigen fibrillarin Silver Sodium Chloride
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Clark M W
Division of Biology, California Institute of Technology, Pasadena 91125.
Yip M L
Campbell J
Abelson J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.5.1741
Published
1990-11-00
Pages
1741-51
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116348
Subset
IM
Grants
NIGMS NIH HHS · 5732 GM 1008-02 · United States
NIGMS NIH HHS · GM 25588 · United States
NIGMS NIH HHS · GM 3267 · United States
External Links
PubMed source DOI: 10.1083/jcb.111.5.1741 PMC Full Text View journal details
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