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PMID: 12024052 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A mammal-specific exon of WT1 is not required for development or fertility.

Molecular and cellular biology ·Vol. 22 ·No. 12 ·2002-06-00 ·Pages 4433-8

Natoli TA, McDonald A, Alberta JA, Taglienti ME, Housman DE, Kreidberg JA

Abstract

The WT1 tumor suppressor gene is a zinc finger-containing transcription factor which is required for development of the kidney and gonads. A mammal-specific alternative splicing event within this gene results in the presence or absence of a 17-amino-acid sequence within the WT1 protein. To determine the function of this sequence in vivo, gene targeting was utilized to specifically eliminate the exon encoding this sequence in mice. Mice lacking WT1 exon 5 develop normally. Adult mice lacking this exon are viable and fertile, and females are capable of lactation.

MeSH Terms
Alternative Splicing Animals Exons Female Fertility/genetics Male Mammals/genetics Mice Mice, Inbred C57BL Mice, Mutant Strains Sperm Count WT1 Proteins/genetics
Chemicals
WT1 Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Natoli Thomas A
Department of Medicine, The Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
McDonald Alice
Alberta Julia A
Taglienti Mary E
Housman David E
Kreidberg Jordan A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.22.12.4433-4438.2002
Published
2002-06-00
Pages
4433-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC133868
Subset
IM
Grants
NIDDK NIH HHS · R01 DK050118 · United States
NIDDK NIH HHS · DK50118 · United States
External Links
PubMed source DOI: 10.1128/MCB.22.12.4433-4438.2002 PMC Full Text View journal details
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