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PMID: 12032092 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A novel regulatory element determines the timing of Mos mRNA translation during Xenopus oocyte maturation.

The EMBO journal ·Vol. 21 ·No. 11 ·2002-06-03 ·Pages 2798-806

Charlesworth A, Ridge JA, King LA, MacNicol MC, MacNicol AM

Abstract

Progression through vertebrate oocyte maturation requires that pre-existing, maternally derived mRNAs be translated in a strict temporal order. The mechanism that controls the timing of oocyte mRNA translation is unknown. In this study we show that the early translational induction of the mRNA encoding the Mos proto-oncogene is mediated through a novel regulatory element within the 3' untranslated region of the Mos mRNA. This novel element is responsive to the MAP kinase signaling pathway and is distinct from the late acting, cdc2-responsive, cytoplasmic polyadenylation element. Our findings suggest that the timing of maternal mRNA translation is controlled through signal transduction pathways targeting distinct 3' UTR mRNA elements.

MeSH Terms
3' Untranslated Regions Animals Base Sequence Blotting, Northern Blotting, Western CDC2 Protein Kinase/metabolism Cytoplasm/metabolism Glutathione Transferase/metabolism MAP Kinase Signaling System Models, Biological Molecular Sequence Data Mutagenesis Oocytes/metabolism Plasmids/metabolism Polyadenylation Progesterone/metabolism Protein Biosynthesis Proto-Oncogene Proteins c-mos/metabolism,physiology RNA/metabolism RNA, Messenger/metabolism Recombinant Fusion Proteins/metabolism Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Time Factors Xenopus/metabolism
Chemicals
3' Untranslated Regions RNA, Messenger Recombinant Fusion Proteins Progesterone RNA Glutathione Transferase Proto-Oncogene Proteins c-mos CDC2 Protein Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Charlesworth Amanda
Department of Anatomy and Neurobiology, University of Arkansas for Medical Sciences, 4301 West Markham Street Slot 814, Little Rock, AR 72205, USA.
Ridge John A
King Leslie A
MacNicol Melanie C
MacNicol Angus M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/21.11.2798
Published
2002-06-03
Pages
2798-806
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125381
Subset
IM
Grants
NICHD NIH HHS · R01 HD035688 · United States
NICHD NIH HHS · HD 35688 · United States
External Links
PubMed source DOI: 10.1093/emboj/21.11.2798 PMC Full Text View journal details
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