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PMID: 12134073 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Small GTP-binding protein TC10 differentially regulates two distinct populations of filamentous actin in 3T3L1 adipocytes.

Molecular biology of the cell ·Vol. 13 ·No. 7 ·2002-07-00 ·Pages 2334-46

Kanzaki M, Watson RT, Hou JC, Stamnes M, Saltiel AR, Pessin JE

Abstract

TC10 is a member of the Rho family of small GTP-binding proteins that has previously been implicated in the regulation of insulin-stimulated GLUT4 translocation in adipocytes. In a manner similar to Cdc42-stimulated actin-based motility, we have observed that constitutively active TC10 (TC10/Q75L) can induce actin comet tails in Xenopus oocyte extracts in vitro and extensive actin polymerization in the perinuclear region when expressed in 3T3L1 adipocytes. In contrast, expression of TC10/Q75L completely disrupted adipocyte cortical actin, which was specific for TC10, because expression of constitutively active Cdc42 was without effect. The effect of TC10/Q75L to disrupt cortical actin was abrogated after deletion of the amino terminal extension (DeltaN-TC10/Q75L), whereas this deletion retained the ability to induce perinuclear actin polymerization. In addition, alteration of perinuclear actin by expression of TC10/Q75L, a dominant-interfering TC10/T31N mutant or a mutant N-WASP protein (N-WASP/DeltaVCA) reduced the rate of VSV G protein trafficking to the plasma membrane. Furthermore, TC10 directly bound to Golgi COPI coat proteins through a dilysine motif in the carboxyl terminal domain consistent with a role for TC10 regulating actin polymerization on membrane transport vesicles. Together, these data demonstrate that TC10 can differentially regulate two types of filamentous actin in adipocytes dependent on distinct functional domains and its subcellular compartmentalization.

MeSH Terms
3T3 Cells Actins/metabolism Adipocytes/drug effects,metabolism Amino Acid Motifs Animals Bacterial Proteins Bacterial Toxins/pharmacology Biological Transport/physiology Bridged Bicyclo Compounds, Heterocyclic/pharmacology CHO Cells Cell Nucleus/metabolism Coatomer Protein/metabolism Cricetinae Cytoplasmic Vesicles/metabolism Enzyme Activation Golgi Apparatus/metabolism Mice Microinjections Microscopy, Confocal Mutagenesis, Site-Directed Nerve Tissue Proteins/genetics,metabolism Oocytes/physiology Thiazoles/pharmacology Thiazolidines Time Factors Wiskott-Aldrich Syndrome Protein, Neuronal Xenopus laevis cdc42 GTP-Binding Protein/genetics,metabolism rho GTP-Binding Proteins/genetics,metabolism
Chemicals
Actins Bacterial Proteins Bacterial Toxins Bridged Bicyclo Compounds, Heterocyclic Coatomer Protein Nerve Tissue Proteins Thiazoles Thiazolidines Wasl protein, mouse Wiskott-Aldrich Syndrome Protein, Neuronal toxB protein, Clostridium difficile Rhoq protein, mouse cdc42 GTP-Binding Protein rho GTP-Binding Proteins latrunculin B
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kanzaki Makoto
Department of Physiology and Biophysics, The University of Iowa, Iowa City, Iowa 52242, USA.
Watson Robert T
Hou June Chunqiu
Stamnes Mark
Saltiel Alan R
Pessin Jeffrey E
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.01-10-0490
Published
2002-07-00
Pages
2334-46
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC117317
Subset
IM
Grants
NIDDK NIH HHS · R01 DK059291 · United States
NIDDK NIH HHS · DK-25295 · United States
NIDDK NIH HHS · DK-59291 · United States
External Links
PubMed source DOI: 10.1091/mbc.01-10-0490 PMC Full Text View journal details
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