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PMID: 12215514 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The regulation of actin organization by actin-depolymerizing factor in elongating pollen tubes.

The Plant cell ·Vol. 14 ·No. 9 ·2002-09-00 ·Pages 2175-90

Chen CY, Wong EI, Vidali L, Estavillo A, Hepler PK, Wu HM, Cheung AY

Abstract

Pollen tube elongation is a polarized cell growth process that transports the male gametes from the stigma to the ovary for fertilization inside the ovules. Actomyosin-driven intracellular trafficking and active actin remodeling in the apical and subapical regions of pollen tubes are both important aspects of this rapid tip growth process. Actin-depolymerizing factor (ADF) and cofilin are actin binding proteins that enhance the depolymerization of microfilaments at their minus, or slow-growing, ends. A pollen-specific ADF from tobacco, NtADF1, was used to dissect the role of ADF in pollen tube growth. Overexpression of NtADF1 resulted in the reduction of fine, axially oriented actin cables in transformed pollen tubes and in the inhibition of pollen tube growth in a dose-dependent manner. Thus, the proper regulation of actin turnover by NtADF1 is critical for pollen tube growth. When expressed at a moderate level in pollen tubes elongating in in vitro cultures, green fluorescent protein (GFP)-tagged NtADF1 (GFP-NtADF1) associated predominantly with a subapical actin mesh composed of short actin filaments and with long actin cables in the shank. Similar labeling patterns were observed for GFP-NtADF1-expressing pollen tubes elongating within the pistil. A Ser-6-to-Asp conversion abolished the interaction between NtADF1 and F-actin in elongating pollen tubes and reduced its inhibitory effect on pollen tube growth significantly, suggesting that phosphorylation at Ser-6 may be a prominent regulatory mechanism for this pollen ADF. As with some ADF/cofilin, the in vitro actin-depolymerizing activity of recombinant NtADF1 was enhanced by slightly alkaline conditions. Because a pH gradient is known to exist in the apical region of elongating pollen tubes, it seems plausible that the in vivo actin-depolymerizing activity of NtADF1, and thus its contribution to actin dynamics, may be regulated spatially by differential H(+) concentrations in the apical region of elongating pollen tubes.

MeSH Terms
Actins/genetics,metabolism Amino Acid Sequence Cell Division/physiology Cytoskeleton/metabolism DNA, Complementary/chemistry,genetics Gene Expression Regulation, Plant Green Fluorescent Proteins Lilium/genetics,metabolism Luminescent Proteins/genetics,metabolism Microfilament Proteins/chemistry,genetics,metabolism Molecular Sequence Data Plant Proteins/chemistry,genetics,metabolism Pollen/genetics,growth & development Protein Conformation Sequence Analysis, DNA Sequence Homology, Amino Acid Serine/metabolism Tobacco/genetics,metabolism
Chemicals
Actins DNA, Complementary Luminescent Proteins Microfilament Proteins Plant Proteins Green Fluorescent Proteins Serine
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Chen Christine Y
Molecular and Cell Biology Program, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Wong Eric I
Vidali Luis
Estavillo Athena
Hepler Peter K
Wu Hen-ming
Cheung Alice Y
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.003038
Published
2002-09-00
Pages
2175-90
Language
English
Region
England
NLM ID
9208688
PMCID
PMC150764
Subset
IM
Grants
NIGMS NIH HHS · GM52953 · United States
Databases
GENBANK
AY081941, AY081942
External Links
PubMed source DOI: 10.1105/tpc.003038 PMC Full Text View journal details
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