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PMID: 8425049 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Molecular cloning and characterization of anther-preferential cDNA encoding a putative actin-depolymerizing factor.

Plant molecular biology ·Vol. 21 ·No. 1 ·1993-01-00 ·Pages 39-45

Kim SR, Kim Y, An G

Abstract

A cDNA clone, LMP131A, which is preferentially expressed in mature anther was isolated from a lily cDNA library. Northern blot analysis and plaque hybridization experiments showed that the LMP131A mRNA is present at ca. 0.3% of the mRNA in mature pollen and is not detectable in carpel, petal, floral bud, leaf, or root. The clone contains an open reading frame of 139 amino acid residues which shows greater than 40% sequence identity in a 91 amino acid overlap to animal actin-depolymerizing factors (ADF), cofilin and destrin. The sequences at and near the actin-binding site are most conserved. Using the lily clone as a probe, a cDNA clone, BMP1, was isolated from a mature anther library of Brassica napus. The expression pattern of the BMP1 clone was the same as that of the lily clone. The Brassica anther-preferential clone contains an open reading frame which is 79% identical to the lily LMP131A protein. Southern blot analysis showed that there are one or a few copies of the putative ADF genes in B. napus and Arabidopsis thaliana.

MeSH Terms
Actin Depolymerizing Factors Actins/metabolism Amino Acid Sequence Animals Base Sequence Blotting, Northern Cloning, Molecular/methods DNA/genetics Destrin Gene Library Humans Microfilament Proteins/genetics Molecular Sequence Data Plants/genetics RNA, Messenger/genetics,metabolism Sequence Homology, Amino Acid
Chemicals
Actin Depolymerizing Factors Actins DSTN protein, human Destrin Microfilament Proteins RNA, Messenger DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kim S R
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340.
Kim Y
An G
References (34)
34 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00039616
Published
1993-01-00
Pages
39-45
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
Z14109, Z14110
External Links
PubMed source DOI: 10.1007/BF00039616 View journal details
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