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PMID: 12242347 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The TC10-interacting protein CIP4/2 is required for insulin-stimulated Glut4 translocation in 3T3L1 adipocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 20 ·2002-10-01 ·Pages 12835-40

Chang L, Adams RD, Saltiel AR

Abstract

The GTPase TC10 plays a critical role in insulin-stimulated glucose transport. We report here the identification of the TC10-interacting protein CIP4/2 (Cdc42-interacting protein 4/2) as an effector in this pathway. CIP4/2 localizes to an intracellular compartment under basal conditions and translocates to the plasma membrane on insulin stimulation. Overexpression of constitutively active TC10 brings CIP4/2 to the plasma membrane, whereas overexpression of an inhibitory form of TC10 blocks the translocation of CIP4/2 produced by insulin. Overexpression of mutant forms of CIP4/2 containing an N-terminal deletion or with diminished TC10 binding inhibits insulin-stimulated Glut4 translocation. These data suggest that CIP4/2 may play an important role in insulin-stimulated glucose transport as a downstream effector of TC10.

MeSH Terms
3T3 Cells Animals COS Cells Cell Line Cell Membrane/metabolism Cytoskeleton/metabolism DNA, Complementary/metabolism Gene Deletion Glucose Transporter Type 4 Glutathione Transferase/metabolism Immunoblotting Insulin/metabolism Mice Microscopy, Fluorescence Microtubule-Associated Proteins/metabolism,physiology Minor Histocompatibility Antigens Molecular Sequence Data Monosaccharide Transport Proteins/metabolism Muscle Proteins Precipitin Tests Protein Binding Protein Structure, Tertiary Protein Transport Recombinant Fusion Proteins/metabolism Time Factors Two-Hybrid System Techniques rho GTP-Binding Proteins/metabolism src Homology Domains
Chemicals
DNA, Complementary Glucose Transporter Type 4 Insulin Microtubule-Associated Proteins Minor Histocompatibility Antigens Monosaccharide Transport Proteins Muscle Proteins Recombinant Fusion Proteins Slc2a4 protein, mouse Trip10 protein, mouse Glutathione Transferase Rhoq protein, mouse rho GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chang Louise
Departments of Internal Medicine and Physiology, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.
Adams Rachael D
Saltiel Alan R
References (33)
33 references, click to expand
  1. The role of glucose metabolites in the activation and translocation of glycogen synthase by insulin in 3T3-L1 adipocytes.
    J Biol Chem. 1999 Sep 24;274(39):27497-504 PMID: 10488084
  2. A novel, multifuntional c-Cbl binding protein in insulin receptor signaling in 3T3-L1 adipocytes.
    Mol Cell Biol. 1998 Feb;18(2):872-9 PMID: 9447983
  3. Mutations of the serine phosphorylated in the protein phosphatase-1-binding motif in the skeletal muscle glycogen-targeting subunit.
    Biochem J. 2000 Feb 15;346 Pt 1:77-82 PMID: 10657242
  4. Cdc42-interacting protein 4 mediates binding of the Wiskott-Aldrich syndrome protein to microtubules.
    J Biol Chem. 2000 Mar 17;275(11):7854-61 PMID: 10713100
  5. CAP defines a second signalling pathway required for insulin-stimulated glucose transport.
    Nature. 2000 Sep 14;407(6801):202-7 PMID: 11001060
  6. Spatial compartmentalization of signal transduction in insulin action.
    Bioessays. 2001 Mar;23(3):215-22 PMID: 11223878
  7. Insulin-stimulated GLUT4 translocation requires the CAP-dependent activation of TC10.
    Nature. 2001 Apr 19;410(6831):944-8 PMID: 11309621
  8. The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways.
    Nature. 2001 May 10;411(6834):215-9 PMID: 11346801
  9. The sorbin homology domain: a motif for the targeting of proteins to lipid rafts.
    Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9098-103 PMID: 11481476
  10. Lipid raft microdomain compartmentalization of TC10 is required for insulin signaling and GLUT4 translocation.
    J Cell Biol. 2001 Aug 20;154(4):829-40 PMID: 11502760
  11. Insulin-stimulated GLUT4 translocation in adipocytes is dependent upon cortical actin remodeling.
    J Biol Chem. 2001 Nov 9;276(45):42436-44 PMID: 11546823
  12. Recent improvements to the SMART domain-based sequence annotation resource.
    Nucleic Acids Res. 2002 Jan 1;30(1):242-4 PMID: 11752305
  13. Insulin signaling pathways in time and space.
    Trends Cell Biol. 2002 Feb;12(2):65-71 PMID: 11849969
  14. Cloning and functional characterization of related TC10 isoforms, a subfamily of Rho proteins involved in insulin-stimulated glucose transport.
    J Biol Chem. 2002 Apr 12;277(15):13067-73 PMID: 11821390
  15. APS facilitates c-Cbl tyrosine phosphorylation and GLUT4 translocation in response to insulin in 3T3-L1 adipocytes.
    Mol Cell Biol. 2002 Jun;22(11):3599-609 PMID: 11997497
  16. Small GTP-binding protein TC10 differentially regulates two distinct populations of filamentous actin in 3T3L1 adipocytes.
    Mol Biol Cell. 2002 Jul;13(7):2334-46 PMID: 12134073
  17. SMART, a simple modular architecture research tool: identification of signaling domains.
    Proc Natl Acad Sci U S A. 1998 May 26;95(11):5857-64 PMID: 9600884
  18. Distinct cellular effects and interactions of the Rho-family GTPase TC10.
    Curr Biol. 1998 Oct 22;8(21):1151-60 PMID: 9799731
  19. Identification of inducible genes at the early stage of adipocyte differentiation of 3T3-L1 cells.
    Biochem Biophys Res Commun. 1999 Jan 19;254(2):299-305 PMID: 9918832
  20. Characterization of rac and cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPases.
    J Biol Chem. 1999 May 7;274(19):13198-204 PMID: 10224076
  21. Synip: a novel insulin-regulated syntaxin 4-binding protein mediating GLUT4 translocation in adipocytes.
    Mol Cell. 1999 Jun;3(6):751-60 PMID: 10394363
  22. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  23. Phosphatidylinositol-3-kinase in isolated rat adipocytes. Activation by insulin and subcellular distribution.
    J Biol Chem. 1992 Feb 15;267(5):3423-8 PMID: 1310686
  24. Insulin-stimulated phosphatidylinositol 3-kinase. Association with a 185-kDa tyrosine-phosphorylated protein (IRS-1) and localization in a low density membrane vesicle.
    J Biol Chem. 1993 Feb 25;268(6):4391-8 PMID: 8382701
  25. gp160, a tissue-specific marker for insulin-activated glucose transport.
    Proc Natl Acad Sci U S A. 1994 Aug 16;91(17):8017-21 PMID: 8058750
  26. Cloning and characterization of a novel insulin-regulated membrane aminopeptidase from Glut4 vesicles.
    J Biol Chem. 1995 Oct 6;270(40):23612-8 PMID: 7559527
  27. Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization.
    Cell. 1996 Mar 8;84(5):723-34 PMID: 8625410
  28. N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases.
    EMBO J. 1996 Oct 1;15(19):5326-35 PMID: 8895577
  29. PTG, a protein phosphatase 1-binding protein with a role in glycogen metabolism.
    Science. 1997 Mar 7;275(5305):1475-8 PMID: 9045612
  30. Syntaxin 4, VAMP2, and/or VAMP3/cellubrevin are functional target membrane and vesicle SNAP receptors for insulin-stimulated GLUT4 translocation in adipocytes.
    Mol Cell Biol. 1997 May;17(5):2425-35 PMID: 9111311
  31. Insulin stimulates tyrosine phosphorylation of the proto-oncogene product of c-Cbl in 3T3-L1 adipocytes.
    Biochem J. 1997 Jun 15;324 ( Pt 3):839-45 PMID: 9210408
  32. A Cdc42 target protein with homology to the non-kinase domain of FER has a potential role in regulating the actin cytoskeleton.
    Curr Biol. 1997 Jul 1;7(7):479-87 PMID: 9210375
  33. The structural basis of Rho effector recognition revealed by the crystal structure of human RhoA complexed with the effector domain of PKN/PRK1.
    Mol Cell. 1999 Nov;4(5):793-803 PMID: 10619026
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.202495599
Published
2002-10-01
Epub
2002-00-19
Pages
12835-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC130546
Subset
IM
Grants
NIDDK NIH HHS · R01 DK060591 · United States
NIDDK NIH HHS · R01 DK60591 · United States
Databases
GENBANK
AF502565
External Links
PubMed source DOI: 10.1073/pnas.202495599 PMC Full Text View journal details
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