Home LiteratureArticle Details
PMID: 12271073 Published · ppublish English Journal Article

Activation of K+ Channels in the Plasma Membrane of Arabidopsis by ATP Produced Photosynthetically.

The Plant cell ·Vol. 5 ·No. 4 ·1993-04-00 ·Pages 477-484

Spalding EP, Goldsmith M

Abstract

Light activates a K+ channel and transiently depolarizes the plasma membrane of Arabidopsis mesophyll cells. Genetically or chemically impairing photosynthesis abolished this electrical response to light. These results indicate that illuminated chloroplasts produce a factor that activated K+ channels in the plasma membrane. By patch clamping at the single-channel level, we have obtained evidence that ATP is one such factor. Application of 0.2 to 2 mM ATP to the cytoplasmic side of excised patches of membrane reversibly activated the type of channel that was activated by light in cell-attached patches. In addition, an outward-rectifying K+ channel and an outward-rectifying nonselective cation channel were similarly activated by ATP, whereas a nonselective stretch-activated channel was unaffected by this treatment. This novel mechanism for controlling the permeability of the plasma membrane to K+ may be important to photosynthetic metabolism.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Spalding E. P.
Department of Biology, Yale University, P.O. Box 6666, New Haven, Connecticut 06511.
Goldsmith MHM.
References (25)
25 references, click to expand
  1. Adenylate Levels, Energy Charge, and Phosphorylation Potential during Dark-Light and Light-Dark Transition in Chloroplasts, Mitochondria, and Cytosol of Mesophyll Protoplasts from Avena sativa L.
    Plant Physiol. 1982 Feb;69(2):448-55 PMID: 16662227
  2. Action potentials in Acetabularia: measurement and simulation of voltage-gated fluxes.
    J Membr Biol. 1991 Dec;124(3):265-73 PMID: 1664861
  3. A mechanosensitive channel in whole cells and in membrane patches of the fungus Uromyces.
    Science. 1991 Sep 20;253(5026):1415-7 PMID: 1716786
  4. Red light stimulates an electrogenic proton pump in Vicia guard cell protoplasts.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):436-40 PMID: 2829187
  5. A mechanosensitive ion channel in the yeast plasma membrane.
    Science. 1988 Nov 4;242(4879):762-5 PMID: 2460920
  6. Changes in the intracellular levels of ATP, ADP, AMP and P1 and regulatory function of the adenylate system in leaf cells during photosynthesis.
    Biochim Biophys Acta. 1965 May 25;102(1):39-54 PMID: 5833412
  7. Ion channels in Arabidopsis plasma membrane : transport characteristics and involvement in light-induced voltage changes.
    Plant Physiol. 1992 May;99(1):96-102 PMID: 16668890
  8. A mechanosensitive ion channel in Schizosaccharomyces pombe.
    EMBO J. 1992 Aug;11(8):2869-75 PMID: 1379173
  9. K Channels Are Responsible for an Inwardly Rectifying Current in the Plasma Membrane of Mesophyll Protoplasts of Avena sativa.
    Plant Physiol. 1992 Mar;98(3):1087-97 PMID: 16668731
  10. Stretch-activated chloride, potassium, and calcium channels coexisting in plasma membranes of guard cells of Vicia faba L.
    Planta. 1991 Dec;186(1):143-53 PMID: 11538499
  11. Glycolysis preferentially inhibits ATP-sensitive K+ channels in isolated guinea pig cardiac myocytes.
    Science. 1987 Oct 2;238(4823):67-9 PMID: 2443972
  12. ATP is a coupling modulator of parallel Na,K-ATPase-K-channel activity in the renal proximal tubule.
    Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6418-22 PMID: 1321439
  13. Voltage-dependent ATP-sensitive potassium channels of skeletal muscle membrane.
    Nature. 1985 Aug 22-28;316(6030):736-8 PMID: 2412127
  14. ATP-regulated K+ channels in cardiac muscle.
    Nature. 1983 Sep 8-14;305(5930):147-8 PMID: 6310409
  15. Involvement of ion channels and active transport in osmoregulation and signaling of higher plant cells.
    Trends Biochem Sci. 1989 May;14(5):187-92 PMID: 2475930
  16. Ca2+ and nucleotide dependent regulation of voltage dependent anion channels in the plasma membrane of guard cells.
    EMBO J. 1990 Dec;9(12):3889-92 PMID: 1701140
  17. ATP-Regulated Ion Channels in the Plasma Membrane of a Characeae Alga, Nitellopsis obtusa.
    Plant Physiol. 1990 May;93(1):343-6 PMID: 16667458
  18. Redox Transfer across the Inner Chloroplast Envelope Membrane.
    Plant Physiol. 1991 Apr;95(4):1131-7 PMID: 16668101
  19. Evidence for G-Protein Regulation of Inward K+ Channel Current in Guard Cells of Fava Bean.
    Plant Cell. 1991 Sep;3(9):1037-1044 PMID: 12324626
  20. Cloning and expression in yeast of a plant potassium ion transport system.
    Science. 1992 May 1;256(5057):663-5 PMID: 1585180
  21. Potassium Channels in Motor Cells of Samanea saman: A Patch-Clamp Study.
    Plant Physiol. 1988 Nov;88(3):643-8 PMID: 16666362
  22. Stromal pH and Photosynthesis Are Affected by Electroneutral K and H Exchange through Chloroplast Envelope Ion Channels.
    Plant Physiol. 1992 Feb;98(2):666-72 PMID: 16668693
  23. Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1992 May 1;89(9):3736-40 PMID: 1570292
  24. Ion channel gating in plants: physiological implications and integration for stomatal function.
    J Membr Biol. 1991 Nov;124(2):95-112 PMID: 1662287
  25. The role of ionic currents in establishing developmental pattern.
    Philos Trans R Soc Lond B Biol Sci. 1981 Oct 7;295(1078):553-66 PMID: 6117911
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.5.4.477
Published
1993-04-00
Pages
477-484
Language
English
Region
England
NLM ID
9208688
PMCID
PMC160286
External Links
PubMed source DOI: 10.1105/tpc.5.4.477 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2