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PMID: 1379173 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A mechanosensitive ion channel in Schizosaccharomyces pombe.

The EMBO journal ·Vol. 11 ·No. 8 ·1992-08-00 ·Pages 2869-75

Zhou XL, Kung C

Abstract

Protoplast protuberances (blebs) of Schizosaccharomyces pombe were examined using the patch-clamp technique. In addition to several voltage-gated ion channels, we encountered the activities of a mechanosensitive ion channel with a conductance of 180 pS. Microscopic currents of one or two units were observed in some excised patches and ensemble currents of several tens of units were observed in all blebs examined in whole-bleb configuration. This channel opens at pressures of cm Hg applied to whole blebs and it passes cations, including Ca2+. It is inactivated by membrane depolarizations and blocked by Gd3+. We discuss the possible functions of such a channel, including its activation upon cell cycle dependent cytoskeletal reorganizations.

MeSH Terms
Cell Membrane/physiology Gadolinium/pharmacology Ion Channels/drug effects,physiology Mechanoreceptors/physiology Membrane Potentials Models, Biological Pressure Protoplasts/physiology Schizosaccharomyces/physiology
Chemicals
Ion Channels Gadolinium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zhou X L
Laboratory of Molecular Biology, University of Wisconsin, Madison 53706.
Kung C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-08-00
Pages
2869-75
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556767
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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