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PMID: 12359879 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overexpression of a pattern-recognition receptor, peptidoglycan-recognition protein-LE, activates imd/relish-mediated antibacterial defense and the prophenoloxidase cascade in Drosophila larvae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 21 ·2002-10-15 ·Pages 13705-10

Takehana A, Katsuyama T, Yano T, Oshima Y, Takada H, Aigaki T, Kurata S

Abstract

In Drosophila, microbial infection activates an antimicrobial defense system involving the activation of proteolytic cascades in the hemolymph and intracellular signaling pathways, the immune deficiency (imd) and Toll pathways, in immune-responsive tissues. The mechanisms for microbial recognition are largely unknown. We report that, in larvae, the imd-mediated antibacterial defense is activated by peptidoglycan-recognition protein (PGRP)-LE, a PGRP-family member in Drosophila. Consistent with this, PGRP-LE binds to the diaminopimelic acid-type peptidoglycan, a cell-wall component of the bacteria capable of activating the imd pathway, but not to the lysine-type peptidoglycan. Moreover, PGRP-LE activates the prophenoloxidase cascade, a proteolytic cascade in the hemolymph. Therefore, PGRP-LE acts as a pattern-recognition receptor to the diaminopimelic acid-type peptidoglycan and activates both the proteolytic cascade and intracellular signaling in Drosophila immunity.

MeSH Terms
Animals Base Sequence Carrier Proteins/genetics,immunology,metabolism Catechol Oxidase/metabolism DNA/genetics Drosophila/genetics,growth & development,immunology,metabolism Drosophila Proteins/genetics,immunology,metabolism Enzyme Precursors/metabolism Gene Expression Genes, Insect Larva/immunology,metabolism Peptidoglycan/metabolism Signal Transduction Transcription Factors/genetics,immunology
Chemicals
Carrier Proteins Drosophila Proteins Enzyme Precursors Peptidoglycan Rel protein, Drosophila Transcription Factors imd protein, Drosophila peptidoglycan recognition protein DNA pro-phenoloxidase Catechol Oxidase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Takehana Aya
Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan.
Katsuyama Tomonori
Yano Tamaki
Oshima Yoshiteru
Takada Haruhiko
Aigaki Toshiro
Kurata Shoichiro
References (35)
35 references, click to expand
  1. Relish, a central factor in the control of humoral but not cellular immunity in Drosophila.
    Mol Cell. 1999 Nov;4(5):827-37 PMID: 10619029
  2. Immunoadjuvant activities of peptidoglycan subunits from the cell walls of Staphyloccus aureus and Lactobacillus plantarum.
    Biken J. 1975 Jun;18(2):93-103 PMID: 1180873
  3. A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13772-7 PMID: 11106397
  4. Tissue-specific inducible expression of antimicrobial peptide genes in Drosophila surface epithelia.
    Immunity. 2000 Nov;13(5):737-48 PMID: 11114385
  5. The imd gene is required for local Cecropin expression in Drosophila barrier epithelia.
    EMBO Rep. 2001 Mar;2(3):239-43 PMID: 11266367
  6. Drosophila immunity: two paths to NF-kappaB.
    Trends Immunol. 2001 May;22(5):260-4 PMID: 11323284
  7. Peptidoglycan recognition proteins: a novel family of four human innate immunity pattern recognition molecules.
    J Biol Chem. 2001 Sep 14;276(37):34686-94 PMID: 11461926
  8. The Drosophila immune response against Gram-negative bacteria is mediated by a peptidoglycan recognition protein.
    Nature. 2002 Apr 11;416(6881):640-4 PMID: 11912488
  9. Functional genomic analysis of phagocytosis and identification of a Drosophila receptor for E. coli.
    Nature. 2002 Apr 11;416(6881):644-8 PMID: 11912489
  10. Requirement for a peptidoglycan recognition protein (PGRP) in Relish activation and antibacterial immune responses in Drosophila.
    Science. 2002 Apr 12;296(5566):359-62 PMID: 11872802
  11. Evolution of the lectin-complement pathway and its role in innate immunity.
    Nat Rev Immunol. 2002 May;2(5):346-53 PMID: 12033740
  12. Peptidoglycan types of bacterial cell walls and their taxonomic implications.
    Bacteriol Rev. 1972 Dec;36(4):407-77 PMID: 4568761
  13. Mitogenic effects of bacterial cell walls, their fragments, and related synthetic compounds on thymocytes and splenocytes of guinea pigs.
    Infect Immun. 1979 Aug;25(2):645-52 PMID: 314932
  14. Approaching the asymptote? Evolution and revolution in immunology.
    Cold Spring Harb Symp Quant Biol. 1989;54 Pt 1:1-13 PMID: 2700931
  15. Insect immunity: developmental and inducible activity of the Drosophila diptericin promoter.
    EMBO J. 1992 Apr;11(4):1469-77 PMID: 1373375
  16. Immune reactions in Drosophila and other insects: a model for innate immunity.
    Trends Genet. 1993 May;9(5):178-83 PMID: 8337755
  17. Activation of a Drosophila Janus kinase (JAK) causes hematopoietic neoplasia and developmental defects.
    EMBO J. 1995 Jun 15;14(12):2857-65 PMID: 7796812
  18. Nucleotide sequence of the cDNA encoding the proenzyme of phenol oxidase A1 of Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7769-73 PMID: 7644493
  19. Molecular cloning of insect pro-phenol oxidase: a copper-containing protein homologous to arthropod hemocyanin.
    Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7774-8 PMID: 7644494
  20. A recessive mutation, immune deficiency (imd), defines two distinct control pathways in the Drosophila host defense.
    Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9465-9 PMID: 7568155
  21. The instructive role of innate immunity in the acquired immune response.
    Science. 1996 Apr 5;272(5258):50-3 PMID: 8600536
  22. Purification of a peptidoglycan recognition protein from hemolymph of the silkworm, Bombyx mori.
    J Biol Chem. 1996 Jun 7;271(23):13854-60 PMID: 8662762
  23. The dorsoventral regulatory gene cassette spätzle/Toll/cactus controls the potent antifungal response in Drosophila adults.
    Cell. 1996 Sep 20;86(6):973-83 PMID: 8808632
  24. A modular misexpression screen in Drosophila detecting tissue-specific phenotypes.
    Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12418-22 PMID: 8901596
  25. Drosophila host defense: differential induction of antimicrobial peptide genes after infection by various classes of microorganisms.
    Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14614-9 PMID: 9405661
  26. A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway.
    EMBO J. 1998 Aug 10;17(5):1217-27 PMID: 9482719
  27. New types of clotting factors and defense molecules found in horseshoe crab hemolymph: their structures and functions.
    J Biochem. 1998 Jan;123(1):1-15 PMID: 9504402
  28. Role of the prophenoloxidase-activating system in invertebrate immunity.
    Curr Opin Immunol. 1998 Feb;10(1):23-8 PMID: 9523106
  29. A peptidoglycan recognition protein in innate immunity conserved from insects to humans.
    Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10078-82 PMID: 9707603
  30. The gene search system. A method for efficient detection and rapid molecular identification of genes in Drosophila melanogaster.
    Genetics. 1999 Feb;151(2):725-37 PMID: 9927464
  31. Toll receptor-mediated Drosophila immune response requires Dif, an NF-kappaB factor.
    Genes Dev. 1999 Apr 1;13(7):792-7 PMID: 10197979
  32. Phylogenetic perspectives in innate immunity.
    Science. 1999 May 21;284(5418):1313-8 PMID: 10334979
  33. Constitutive activation of toll-mediated antifungal defense in serpin-deficient Drosophila.
    Science. 1999 Sep 17;285(5435):1917-9 PMID: 10489372
  34. Drosophila Toll is activated by Gram-positive bacteria through a circulating peptidoglycan recognition protein.
    Nature. 2001 Dec 13;414(6865):756-9 PMID: 11742401
  35. The phytopathogenic bacteria Erwinia carotovora infects Drosophila and activates an immune response.
    Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3376-81 PMID: 10725405
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.212301199
Published
2002-10-15
Epub
2002-00-01
Pages
13705-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC129750
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.212301199 PMC Full Text View journal details
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