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PMID: 12361981 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Higher order arrangement of the eukaryotic nuclear bodies.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 21 ·2002-10-15 ·Pages 13583-8

Wang IF, Reddy NM, Shen CK

Abstract

The nuclei of eukaryotic cells consist of discrete substructures. These substructures include the nuclear bodies, which have been implicated in a number of biological processes such as transcription and splicing. However, for most nuclear bodies, the details of involvement in these processes in relation to their three-dimensional distributions in the nucleus are still unclear. Through the analysis of TDP, a protein functional in both transcriptional repression and alternative splicing, we have identified a new category of nuclear bodies within which the TDP molecules reside. Remarkably, the TDP bodies (TBs) colocalize or overlap with several different types of nuclear bodies previously suggested to function in transcription or splicing. Of these nuclear bodies, the Gemini of coiled bodies (GEM) seems to associate with TB through the interaction between survival motor neuron (SMN) protein and TDP. Furthermore, TB sometimes appears to be the bridge of two or more of these other nuclear bodies. Our data suggest the existence of a hierarchy and possibly functional arrangement of the nuclear bodies within the eukaryotic nuclei.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Compartmentation Cell Nucleus Structures/metabolism,ultrastructure Coiled Bodies/metabolism,ultrastructure DNA, Complementary/genetics DNA-Binding Proteins/genetics,metabolism Humans Mice Models, Biological Molecular Sequence Data Nuclear Proteins/genetics,metabolism Protein Isoforms/genetics,metabolism RNA Splicing Recombinant Fusion Proteins/genetics,metabolism Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
DNA, Complementary DNA-Binding Proteins Nuclear Proteins Protein Isoforms Recombinant Fusion Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang I-Fan
Institute of Molecular Biology, Academia Sinica, National Taiwan University, Taipei, Taiwan 115, Republic of China.
Reddy Narsa M
Shen C-K James
References (34)
34 references, click to expand
  1. Identification and characterization of a spinal muscular atrophy-determining gene.
    Cell. 1995 Jan 13;80(1):155-65 PMID: 7813012
  2. Macromolecular domains within the cell nucleus.
    Annu Rev Cell Biol. 1993;9:265-315 PMID: 8280462
  3. Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs.
    J Virol. 1995 Jun;69(6):3584-96 PMID: 7745706
  4. Nuclear domains and the nuclear matrix.
    Int Rev Cytol. 1995;162A:151-89 PMID: 8575880
  5. The RNA 3' cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA.
    EMBO J. 1996 Jun 3;15(11):2883-92 PMID: 8654386
  6. A novel nuclear structure containing the survival of motor neurons protein.
    EMBO J. 1996 Jul 15;15(14):3555-65 PMID: 8670859
  7. Structure and function in the nucleus.
    Science. 1998 Apr 24;280(5363):547-53 PMID: 9554838
  8. Sex-lethal interactions with protein and RNA. Roles of glycine-rich and RNA binding domains.
    J Biol Chem. 1997 Aug 29;272(35):22227-35 PMID: 9268369
  9. Dynamic relocation of transcription and splicing factors dependent upon transcriptional activity.
    EMBO J. 1997 Mar 17;16(6):1401-12 PMID: 9135155
  10. Localization of nascent RNA and CREB binding protein with the PML-containing nuclear body.
    Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4991-6 PMID: 9560216
  11. The cellular organization of gene expression.
    Curr Opin Cell Biol. 1998 Jun;10(3):323-31 PMID: 9640532
  12. A novel function for SMN, the spinal muscular atrophy disease gene product, in pre-mRNA splicing.
    Cell. 1998 Nov 25;95(5):615-24 PMID: 9845364
  13. The Drosophila melanogaster 60A chromosomal division is extremely dense with functional genes: their sequences, genomic organization, and expression.
    Genomics. 1999 Apr 1;57(1):43-56 PMID: 10191082
  14. Nuclear bodies: multifaceted subdomains of the interchromatin space.
    Trends Cell Biol. 1999 Aug;9(8):302-9 PMID: 10407409
  15. Purification and biochemical characterization of interchromatin granule clusters.
    EMBO J. 1999 Aug 2;18(15):4308-20 PMID: 10428969
  16. Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins.
    Hum Mol Genet. 2000 Aug 12;9(13):1977-86 PMID: 10942426
  17. Transcriptional repression by Drosophila methyl-CpG-binding proteins.
    Mol Cell Biol. 2000 Oct;20(19):7401-9 PMID: 10982856
  18. Identification of a nuclear domain with deacetylase activity.
    Proc Natl Acad Sci U S A. 2000 Sep 12;97(19):10330-5 PMID: 10984530
  19. Structure and dynamics of hnRNP-labelled nuclear bodies induced by stress treatments.
    J Cell Sci. 2000 Nov;113 ( Pt 22):4043-53 PMID: 11058091
  20. Regulation of p53 activity in nuclear bodies by a specific PML isoform.
    EMBO J. 2000 Nov 15;19(22):6185-95 PMID: 11080164
  21. In vivo analysis of Cajal body movement, separation, and joining in live human cells.
    J Cell Biol. 2000 Dec 25;151(7):1561-74 PMID: 11134083
  22. An RNA recognition motif (RRM) is required for the localization of PTB-associated splicing factor (PSF) to subnuclear speckles.
    Exp Cell Res. 2001 Feb 1;263(1):131-44 PMID: 11161712
  23. Protein dynamics: implications for nuclear architecture and gene expression.
    Science. 2001 Feb 2;291(5505):843-7 PMID: 11225636
  24. Chromosome territories, nuclear architecture and gene regulation in mammalian cells.
    Nat Rev Genet. 2001 Apr;2(4):292-301 PMID: 11283701
  25. Nuclear factor TDP-43 and SR proteins promote in vitro and in vivo CFTR exon 9 skipping.
    EMBO J. 2001 Apr 2;20(7):1774-84 PMID: 11285240
  26. Characterization and functional implications of the RNA binding properties of nuclear factor TDP-43, a novel splicing regulator of CFTR exon 9.
    J Biol Chem. 2001 Sep 28;276(39):36337-43 PMID: 11470789
  27. Paraspeckles: a novel nuclear domain.
    Curr Biol. 2002 Jan 8;12(1):13-25 PMID: 11790299
  28. The role of PML in tumor suppression.
    Cell. 2002 Jan 25;108(2):165-70 PMID: 11832207
  29. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus.
    Nature. 1990 Feb 1;343(6257):437-41 PMID: 2137203
  30. Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin.
    J Exp Med. 1991 Jun 1;173(6):1407-19 PMID: 2033369
  31. In vivo detection of snRNP-rich organelles in the nuclei of mammalian cells.
    EMBO J. 1991 Jul;10(7):1863-73 PMID: 1710980
  32. Higher level organization of individual gene transcription and RNA splicing.
    Science. 1993 Feb 26;259(5099):1326-30 PMID: 8446901
  33. hnRNP proteins and the biogenesis of mRNA.
    Annu Rev Biochem. 1993;62:289-321 PMID: 8352591
  34. Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains.
    J Cell Biol. 1995 Apr;129(2):287-98 PMID: 7536746
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.212483099
Published
2002-10-15
Epub
2002-00-02
Pages
13583-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC129717
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.212483099 PMC Full Text View journal details
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