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PMID: 12417702 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

AtOPT3, a member of the oligopeptide transporter family, is essential for embryo development in Arabidopsis.

The Plant cell ·Vol. 14 ·No. 11 ·2002-11-00 ·Pages 2799-811

Stacey MG, Koh S, Becker J, Stacey G

Abstract

A T-DNA-tagged population of Arabidopsis was screened for mutations in AtOPT3, which encodes a member of the oligopeptide (OPT) family of peptide transporters, and a recessive mutant allele, opt3, was identified. Phenotypic analysis of opt3 showed that most homozygous embryos were arrested at or before the octant stage of embryo development and that none showed the usual periclinal division leading to the formation of the protoderm. This defective phenotype could be reversed by complementation with the full-length, wild-type AtOPT3 gene. A beta-glucuronidase (GUS) fusion to DNA sequences upstream of the putative AtOPT3 ATG start codon was constructed, and the expression pattern was assayed in transgenic plants. AtOPT3 was expressed in the vascular tissues of seedlings and mature plants as well as in pollen. Consistent with the function of AtOPT3 in embryogenesis, AtOPT3::GUS expression also was detected in developing embryos and in the maternal tissues of seeds. These data suggest a critical role for peptide transport in early embryo development.

MeSH Terms
Alleles Amino Acid Transport Systems/genetics,metabolism Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Carrier Proteins/genetics,metabolism Cell Division/genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genetic Complementation Test Mutation Phenotype Plants, Genetically Modified Pollen/genetics,growth & development,metabolism Seeds/genetics,growth & development,metabolism
Chemicals
Amino Acid Transport Systems Arabidopsis Proteins Carrier Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stacey Minviluz G
Department of Plant Microbiology and Pathology, University of Missouri, Columbia, Missouri 65211, USA.
Koh Serry
Becker Jeffrey
Stacey Gary
References (62)
62 references, click to expand
  1. Human intestinal H+/peptide cotransporter. Cloning, functional expression, and chromosomal localization.
    J Biol Chem. 1995 Mar 24;270(12):6456-63 PMID: 7896779
  2. TAP1-dependent peptide translocation in vitro is ATP dependent and peptide selective.
    Cell. 1993 Aug 13;74(3):577-84 PMID: 8348620
  3. The role of plant peptides in intercellular signalling.
    Curr Opin Plant Biol. 1999 Oct;2(5):365-8 PMID: 10508757
  4. An oligopeptide transport gene from Candida albicans.
    Microbiology. 1997 Feb;143 ( Pt 2):387-96 PMID: 9043116
  5. An oligopeptide transporter gene family in Arabidopsis.
    Plant Physiol. 2002 Jan;128(1):21-9 PMID: 11788749
  6. Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana.
    Genetics. 2001 Dec;159(4):1751-63 PMID: 11779812
  7. Phytosulfokine, sulfated peptides that induce the proliferation of single mesophyll cells of Asparagus officinalis L.
    Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7623-7 PMID: 8755525
  8. Peptide signals and their receptors in higher plants.
    Trends Plant Sci. 2001 Dec;6(12):573-7 PMID: 11738382
  9. An embryo-defective mutant of arabidopsis disrupted in the final step of biotin synthesis
    Plant Physiol. 1998 Mar;116(3):935-46 PMID: 9501126
  10. Cloning of an Arabidopsis histidine transporting protein related to nitrate and peptide transporters.
    FEBS Lett. 1994 Jun 27;347(2-3):185-9 PMID: 8033999
  11. Embryogenesis in Higher Plants: An Overview.
    Plant Cell. 1993 Oct;5(10):1361-1369 PMID: 12271035
  12. Apical-basal pattern formation in Arabidopsis embryogenesis.
    EMBO J. 2001 Jul 16;20(14):3609-16 PMID: 11447101
  13. Peptide transport in plants.
    Trends Plant Sci. 2002 Jun;7(6):257-63 PMID: 12049922
  14. The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation.
    Plant Mol Biol. 1994 Sep;25(6):989-94 PMID: 7919218
  15. Genetic and molecular characterization of embryonic mutants identified following seed transformation in Arabidopsis.
    Mol Gen Genet. 1993 Dec;241(5-6):504-14 PMID: 8264525
  16. Peptide transport by germinating barley embryos: Uptake of physiological di- and oligopeptides.
    Planta. 1978 Jan;138(3):211-5 PMID: 24414048
  17. Multidrug transporters in prokaryotic and eukaryotic cells: physiological functions and transport mechanisms.
    Mol Membr Biol. 2001 Jan-Mar;18(1):97-103 PMID: 11396617
  18. Gamma-glutamyl transpeptidase from kidney bean fruit. I. Purification and mechanism of action.
    Biochim Biophys Acta. 1967 Jan 11;132(1):15-26 PMID: 6030345
  19. Peptides and ATP binding cassette peptide transporters.
    Res Microbiol. 2001 Apr-May;152(3-4):245-58 PMID: 11421272
  20. Suspensor-derived polyembryony caused by altered expression of valyl-tRNA synthetase in the twn2 mutant of Arabidopsis.
    Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7349-55 PMID: 9207094
  21. The barley scutellar peptide transporter: biochemical characterization and localization to the plasma membrane.
    J Exp Bot. 2000 Jul;51(348):1201-9 PMID: 10937695
  22. H+ coupled active transport of bestatin via the dipeptide transport system in rabbit intestinal brush-border membranes.
    J Pharmacol Exp Ther. 1992 Feb;260(2):482-6 PMID: 1738097
  23. Cloning of a Candida albicans peptide transport gene.
    Microbiology. 1995 May;141 ( Pt 5):1147-56 PMID: 7773409
  24. Expression cloning of a mammalian proton-coupled oligopeptide transporter.
    Nature. 1994 Apr 7;368(6471):563-6 PMID: 8139693
  25. cDNA structure, genomic organization, and promoter analysis of the mouse intestinal peptide transporter PEPT1.
    Biochim Biophys Acta. 2000 Jun 21;1492(1):145-54 PMID: 11004485
  26. Multiplicity and regulation of genes encoding peptide transporters in Saccharomyces cerevisiae.
    Mol Membr Biol. 2001 Jan-Mar;18(1):105-12 PMID: 11396605
  27. Quorum sensing: a population-density component in the determination of bacterial phenotype.
    Trends Biochem Sci. 1996 Jun;21(6):214-9 PMID: 8744355
  28. TRANSPORTERS RESPONSIBLE FOR THE UPTAKE AND PARTITIONING OF NITROGENOUS SOLUTES.
    Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:659-688 PMID: 11337412
  29. Embryo-lethal mutants of Arabidopsis thaliana: Evidence for gametophytic expression of the mutant genes.
    Theor Appl Genet. 1982 Dec;63(4):381-6 PMID: 24270876
  30. Embryonic Lethals and T-DNA Insertional Mutagenesis in Arabidopsis.
    Plant Cell. 1991 Feb;3(2):149-157 PMID: 12324593
  31. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
    EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
  32. In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration.
    Methods Mol Biol. 1998;82:259-66 PMID: 9664431
  33. The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein.
    Nature. 1989 Aug 3;340(6232):400-4 PMID: 2569166
  34. Embryo-lethal mutants of Arabidopsis thaliana. A model system for genetic analysis of plant embryo development.
    Dev Biol. 1979 Sep;72(1):50-61 PMID: 510780
  35. Condensin and cohesin knockouts in Arabidopsis exhibit a titan seed phenotype.
    Plant J. 2002 Feb;29(4):405-15 PMID: 11846874
  36. The axr6 mutants of Arabidopsis thaliana define a gene involved in auxin response and early development.
    Development. 2000 Jan;127(1):23-32 PMID: 10654597
  37. Enkephalins are transported by a novel eukaryotic peptide uptake system.
    J Biol Chem. 2000 Feb 4;275(5):3037-41 PMID: 10652283
  38. H+ gradient-dependent transport of aminocephalosporins in rat intestinal brush-border membrane vesicles. Role of dipeptide transport system.
    Biochem Pharmacol. 1986 Jun 1;35(11):1781-6 PMID: 3718527
  39. Developmental control of H+/amino acid permease gene expression during seed development of Arabidopsis.
    Plant J. 1998 Jun;14(5):535-44 PMID: 9675899
  40. The titan mutants of Arabidopsis are disrupted in mitosis and cell cycle control during seed development.
    Plant J. 1998 Oct;16(1):21-31 PMID: 9807824
  41. Polarity and signalling in plant embryogenesis.
    J Exp Bot. 2000 Jun;51(347):971-83 PMID: 10948225
  42. Isolation and characterization of a Saccharomyces cerevisiae peptide transport gene.
    Mol Cell Biol. 1994 Jan;14(1):104-15 PMID: 8264579
  43. Antisense expression of the peptide transport gene AtPTR2-B delays flowering and arrests seed development in transgenic Arabidopsis plants.
    Plant Physiol. 1997 Jul;114(3):927-35 PMID: 9232875
  44. H+ coupled uphill transport of aminocephalosporins via the dipeptide transport system in rabbit intestinal brush-border membranes.
    J Biol Chem. 1986 Oct 25;261(30):14130-4 PMID: 3021727
  45. Peptides: new signalling molecules in plants.
    Trends Plant Sci. 2002 Feb;7(2):78-83 PMID: 11832279
  46. ABP1 is required for organized cell elongation and division in Arabidopsis embryogenesis.
    Genes Dev. 2001 Apr 1;15(7):902-11 PMID: 11297513
  47. Cloning of a second Arabidopsis peptide transport gene.
    Plant Physiol. 1996 Jan;110(1):171-8 PMID: 8587981
  48. Cytokinesis in the Arabidopsis embryo involves the syntaxin-related KNOLLE gene product.
    Cell. 1996 Jan 12;84(1):61-71 PMID: 8548827
  49. Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells.
    EMBO J. 1989 Dec 20;8(13):3973-84 PMID: 2686977
  50. Selective and ATP-dependent translocation of peptides by the MHC-encoded transporter.
    Science. 1993 Aug 6;261(5122):769-71 PMID: 8342042
  51. Inactivation of a glycyl-tRNA synthetase leads to an arrest in plant embryo development.
    Plant Cell. 1998 Aug;10(8):1277-94 PMID: 9707529
  52. Requirements for peptide binding to the human transporter associated with antigen processing revealed by peptide scans and complex peptide libraries.
    J Biol Chem. 1995 Aug 4;270(31):18512-6 PMID: 7543103
  53. Schizosaccharomyces pombe isp4 encodes a transporter representing a novel family of oligopeptide transporters.
    Mol Microbiol. 1998 May;28(4):729-41 PMID: 9643541
  54. Hgt1p, a high affinity glutathione transporter from the yeast Saccharomyces cerevisiae.
    J Biol Chem. 2000 May 5;275(18):13259-65 PMID: 10788431
  55. Cloning and functional characterisation of a peptide transporter expressed in the scutellum of barley grain during the early stages of germination.
    Plant J. 1998 Jul;15(2):221-9 PMID: 9721680
  56. The PTR family: a new group of peptide transporters.
    Mol Microbiol. 1995 Jun;16(5):825-34 PMID: 7476181
  57. An embryo-lethal mutant of Arabidopsis thaliana is a biotin auxotroph.
    Dev Biol. 1989 Jan;131(1):161-7 PMID: 2909401
  58. An Arabidopsis peptide transporter is a member of a new class of membrane transport proteins.
    Plant Cell. 1994 Sep;6(9):1289-99 PMID: 7919993
  59. Diversity of TITAN functions in Arabidopsis seed development.
    Plant Physiol. 2002 Jan;128(1):38-51 PMID: 11788751
  60. The TITAN5 gene of Arabidopsis encodes a protein related to the ADP ribosylation factor family of GTP binding proteins.
    Plant Cell. 2000 Aug;12(8):1379-92 PMID: 10948257
  61. Differential recognition of beta -lactam antibiotics by intestinal and renal peptide transporters, PEPT 1 and PEPT 2.
    J Biol Chem. 1995 Oct 27;270(43):25672-7 PMID: 7592745
  62. A critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana.
    Genes Dev. 2000 Jun 15;14(12):1485-97 PMID: 10859167
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.005629
Published
2002-11-00
Pages
2799-811
Language
English
Region
England
NLM ID
9208688
PMCID
PMC152728
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.005629 PMC Full Text View journal details
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