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PMID: 12477786 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Hyphal tip-associated localization of Cdc42 is F-actin dependent in Candida albicans.

Eukaryotic cell ·Vol. 1 ·No. 6 ·2002-12-00 ·Pages 856-64

Hazan I, Liu H

Abstract

The rho-type GTPase Cdc42 is important for the establishment and maintenance of eukaryotic cell polarity. To examine whether Cdc42 is regulated during the yeast-to-hypha transition in Candida albicans, we constructed a green fluorescence protein (GFP)-Cdc42 fusion under the ACT1 promoter and observed its localization in live C. albicans cells. As in Saccharomyces cerevisiae, GFP-Cdc42 was observed around the entire periphery of the cell. In yeast-form cells of C. albicans, it clustered to the tips and sides of small buds as well as to the mother-daughter neck region of large-budded cells. Upon hyphal induction, GFP-Cdc42 clustered to the site of hyphal evagination and remained at the tips of the hyphae. This temporal and spatial localization of Cdc42 suggests that its activity is regulated during the yeast-to-hypha transition. In addition to the accumulation at the hyphal tip, GFP-Cdc42 was also seen as a band within the hyphal tube in cells that had undergone nuclear separation. With the F-actin-assembly inhibitor latrunculin A, we found that GFP-Cdc42 accumulation at the bud site in yeast-form cells is F-actin independent, whereas GFP-Cdc42 accumulation at the hyphal tip requires F-actin. Furthermore, disruption of the F-actin cytoskeleton impaired the transcriptional induction of hypha-specific genes. Therefore, hypha formation resembles mating in Saccharomyces cerevisiae in that both require F-actin for GFP-Cdc42 localization and efficient signaling.

MeSH Terms
Actins/metabolism Blotting, Northern Bridged Bicyclo Compounds, Heterocyclic/pharmacology Candida albicans/metabolism Cell Cycle Cytoskeleton/metabolism DNA Primers/pharmacology Genes, Fungal Green Fluorescent Proteins Hyphae/metabolism Luminescent Proteins/metabolism Marine Toxins/pharmacology Models, Genetic Plasmids/metabolism Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/metabolism Signal Transduction Thiazoles/pharmacology Thiazolidines Time Factors Transcription, Genetic cdc42 GTP-Binding Protein/chemistry,metabolism
Chemicals
Actins Bridged Bicyclo Compounds, Heterocyclic DNA Primers Luminescent Proteins Marine Toxins Recombinant Fusion Proteins Thiazoles Thiazolidines Green Fluorescent Proteins cdc42 GTP-Binding Protein latrunculin A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hazan Idit
Department of Biological Chemistry, University of California-Irvine, Irvine, California 92697-1700, USA.
Liu Haoping
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Article Info
Journal
Eukaryotic cell
Abbr.
Eukaryot Cell
ISSN
1535-9778
DOI
10.1128/EC.1.6.856-864.2002
Published
2002-12-00
Pages
856-64
Language
English
Region
United States
NLM ID
101130731
PMCID
PMC138748
Subset
IM
Grants
NIGMS NIH HHS · R01 GM055155 · United States
NIGMS NIH HHS · GM-55155 · United States
External Links
PubMed source DOI: 10.1128/EC.1.6.856-864.2002 PMC Full Text View journal details
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