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PMID: 10477756 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The role of actin in spindle orientation changes during the Saccharomyces cerevisiae cell cycle.

The Journal of cell biology ·Vol. 146 ·No. 5 ·1999-09-06 ·Pages 1019-32

Theesfeld CL, Irazoqui JE, Bloom K, Lew DJ

Abstract

In the budding yeast Saccharomyces cerevisiae, the mitotic spindle must align along the mother-bud axis to accurately partition the sister chromatids into daughter cells. Previous studies showed that spindle orientation required both astral microtubules and the actin cytoskeleton. We now report that maintenance of correct spindle orientation does not depend on F-actin during G2/M phase of the cell cycle. Depolymerization of F-actin using Latrunculin-A did not perturb spindle orientation after this stage. Even an early step in spindle orientation, the migration of the spindle pole body (SPB), became actin-independent if it was delayed until late in the cell cycle. Early in the cell cycle, both SPB migration and spindle orientation were very sensitive to perturbation of F-actin. Selective disruption of actin cables using a conditional tropomyosin double-mutant also led to defects in spindle orientation, even though cortical actin patches were still polarized. This suggests that actin cables are important for either guiding astral microtubules into the bud or anchoring them in the bud. In addition, F-actin was required early in the cell cycle for the development of the actin-independent spindle orientation capability later in the cell cycle. Finally, neither SPB migration nor the switch from actin-dependent to actin-independent spindle behavior required B-type cyclins.

MeSH Terms
Actins/metabolism Anaphase/drug effects Bridged Bicyclo Compounds, Heterocyclic/pharmacology CDC28 Protein Kinase, S cerevisiae/genetics,metabolism Calcium-Binding Proteins/genetics,physiology Cell Cycle/drug effects Cell Cycle Proteins/genetics,physiology Cell Polarity/drug effects Cyclin B/genetics,physiology Dyneins/genetics,physiology Fungal Proteins/genetics,physiology Genes, Fungal/genetics,physiology Hydroxyurea/pharmacology Microfilament Proteins Microtubules/drug effects,metabolism Mutation Nocodazole/pharmacology Nuclear Proteins/genetics,physiology Saccharomyces cerevisiae/cytology,drug effects,genetics,metabolism Saccharomyces cerevisiae Proteins Spindle Apparatus/drug effects,metabolism Temperature Thiazoles/pharmacology Thiazolidines Tropomyosin/genetics,metabolism
Chemicals
Actins Bni1 protein, S cerevisiae Bridged Bicyclo Compounds, Heterocyclic CDC31 protein, S cerevisiae Calcium-Binding Proteins Cell Cycle Proteins Cyclin B Fungal Proteins KAR9 protein, S cerevisiae Microfilament Proteins Nuclear Proteins Saccharomyces cerevisiae Proteins Thiazoles Thiazolidines Tropomyosin CDC28 Protein Kinase, S cerevisiae Dyneins Nocodazole latrunculin A Hydroxyurea
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Theesfeld C L
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Irazoqui J E
Bloom K
Lew D J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.146.5.1019
Published
1999-09-06
Pages
1019-32
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2169477
Subset
IM
Grants
NIGMS NIH HHS · R01 GM032238 · United States
NIGMS NIH HHS · GM53050 · United States
External Links
PubMed source DOI: 10.1083/jcb.146.5.1019 PMC Full Text View journal details
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