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PMID: 12513122 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Rhythmicity of engraftment and altered cell cycle kinetics of cytokine-cultured murine marrow in simulated microgravity compared with static cultures.

In vitro cellular & developmental biology. Animal ·Vol. 38 ·No. 6 ·2002-06-00 ·Pages 343-51

Colvin GA, Lambert JF, Carlson JE, McAuliffe CI, Abedi M, Quesenberry PJ

Abstract

Space flight with associated microgravity is complicated by "astronaut's anemia" and other hematologic abnormalities. Altered erythroid differentiation, red cell survival, plasma volume, and progenitor numbers have been reported. We studied the impact of microgravity on engraftable stem cells, culturing marrow cells in rotary wall vessel (RWV) culture chambers mimicking microgravity and in normal gravity nonadherent Teflon bottles. A quantitative competitive engraftment technique was assessed under both conditions in lethally irradiated hosts. We assessed 8-wk engraftable stem cells over a period spanning at least one cell cycle for cytokine (FLT-3 ligand, thrombopoietin [TPO], steel factor)-activated marrow stem cells. Engraftable stem cells were supported out to 56 h under microgravity conditions, and this support was superior to that seen in normal-gravity Teflon bottle cultures out to 40 h, with Teflon bottle culture support superior to RWV from 40 to 56 h. A nadir of stem cell number was seen at 40 h in Teflon and 48 h in RWV, suggesting altered marrow stem cell cycle kinetics under microgravity. This is the first study of engraftable stem cells under microgravity conditions, and the differences between microgravity and normal gravity cultures may present opportunities for unique future stem cell expansion strategies.

MeSH Terms
Animals Bioreactors Bone Marrow Cells Bone Marrow Transplantation Cell Culture Techniques/instrumentation,methods Cell Cycle/physiology Cell Differentiation/physiology Cell Division/physiology Graft Survival Kinetics Mice Mice, Inbred BALB C Rotation Stem Cells/cytology,drug effects,radiation effects Weightlessness Simulation
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Colvin Gerald A
University of Massachusetts Cancer Center, Worcester, MA 01655, USA.
Lambert Jean-François
Carlson Jane E
McAuliffe Christina I
Abedi Mehrdad
Quesenberry Peter J
References (58)
58 references, click to expand
  1. Structure of the chromosomal gene for murine interleukin 3.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):316-20 PMID: 3918308
  2. Induction of carcinoembryonic antigen expression in a three-dimensional culture system.
    In Vitro Cell Dev Biol Anim. 1997 May;33(5):352-7 PMID: 9196893
  3. The endocrine system in space flight.
    Acta Astronaut. 1988;17(2):161-6 PMID: 11537094
  4. Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant.
    Cancer Genet Cytogenet. 1992 Dec;64(2):145-8 PMID: 1486564
  5. Y-encoded, species-specific DNA in mice: evidence that the Y chromosome exists in two polymorphic forms in inbred strains.
    Cell. 1984 May;37(1):171-7 PMID: 6722869
  6. Hematological measurements in rats flown on Spacelab shuttle, SL-3.
    Am J Physiol. 1987 Feb;252(2 Pt 2):R216-21 PMID: 3812759
  7. Anemia of spaceflight.
    Blood. 1982 Nov;60(5):1059-67 PMID: 7126864
  8. Effect of spaceflight on human stem cell hematopoiesis: suppression of erythropoiesis and myelopoiesis.
    J Leukoc Biol. 1996 Jul;60(1):69-76 PMID: 8699125
  9. Density dependent inhibition of cell growth in culture.
    Nature. 1967 Jul 8;215(5097):171-2 PMID: 6049107
  10. Responses of gravity level variations on the NASA/JSC bioreactor system.
    Physiologist. 1992 Feb;35(1 Suppl):S49-50 PMID: 1589533
  11. Tissue culture in microgravity.
    Sci Med (Phila). 1997 May-Jun;4(3):46-55 PMID: 11540729
  12. Three-dimensional endothelial-tumor epithelial cell interactions in human cervical cancers.
    In Vitro Cell Dev Biol Anim. 1997 Jun;33(6):432-42 PMID: 9201511
  13. Effects of microgravity and increased gravity on bone marrow of rats.
    Aviat Space Environ Med. 1994 Aug;65(8):730-5 PMID: 7980333
  14. Conditions determining initiation of DNA synthesis in 3T3 cells.
    Proc Natl Acad Sci U S A. 1970 May;66(1):204-10 PMID: 5273897
  15. Cell adhesion in animal cell culture: physiological and fluid-mechanical implications.
    Bioprocess Technol. 1995;20:61-110 PMID: 7765637
  16. Control of red blood cell mass in spaceflight.
    J Appl Physiol (1985). 1996 Jul;81(1):98-104 PMID: 8828651
  17. Cultivation of fall armyworm ovary cells in simulated microgravity.
    In Vitro Cell Dev Biol Anim. 1997 May;33(5):332-6 PMID: 9196890
  18. Three-dimensional culture of bovine chondrocytes in rotating-wall vessels.
    In Vitro Cell Dev Biol Anim. 1997 May;33(5):358-65 PMID: 9196894
  19. Select de novo gene and protein expression during renal epithelial cell culture in rotating wall vessels is shear stress dependent.
    J Membr Biol. 1999 Mar 1;168(1):77-89 PMID: 10051691
  20. Erythroid cell growth and differentiation in vitro in the simulated microgravity environment of the NASA rotating wall vessel bioreactor.
    In Vitro Cell Dev Biol Anim. 2001 Feb;37(2):79-83 PMID: 11332741
  21. Use of a photolabeling technique to identify nonviable cells in fixed homologous or heterologous cell populations.
    Cytometry. 1991;12(2):133-9 PMID: 2049970
  22. Fabrication, characterization and evaluation of bioceramic hollow microspheres used as microcarriers for 3-D bone tissue formation in rotating bioreactors.
    Biomaterials. 1999 Jun;20(11):989-1001 PMID: 10378799
  23. Thy-1 antigen expression by murine high-proliferative capacity hematopoietic progenitor cells. I. Relation between sensitivity to depletion by Thy-1 antibody and stem cell generation potential.
    J Immunol. 1984 Dec;133(6):2940-9 PMID: 6149245
  24. Cardiac tissue engineering: cell seeding, cultivation parameters, and tissue construct characterization.
    Biotechnol Bioeng. 1999 Sep 5;64(5):580-9 PMID: 10404238
  25. Neonatal rat heart cells cultured in simulated microgravity.
    In Vitro Cell Dev Biol Anim. 1997 May;33(5):337-43 PMID: 9196891
  26. Cell cycle analysis and synchronization of pluripotent hematopoietic progenitor stem cells.
    Blood. 1997 Sep 15;90(6):2293-9 PMID: 9310480
  27. Cultures of human liver cells in simulated microgravity environment.
    Adv Space Res. 1999;24(6):829-36 PMID: 11542629
  28. Use of microgravity bioreactors for development of an in vitro rat salivary gland cell culture model.
    J Cell Biochem. 1993 Mar;51(3):265-73 PMID: 8501128
  29. Skeletal muscle satellite cells cultured in simulated microgravity.
    In Vitro Cell Dev Biol Anim. 1997 May;33(5):386-91 PMID: 9196898
  30. Microgravity culture conditions decrease immunogenicity but maintain excellent morphology of pancreatic islets.
    Transplant Proc. 2001 Feb-Mar;33(1-2):388 PMID: 11266875
  31. Long term organ culture of human prostate tissue in a NASA-designed rotating wall bioreactor.
    J Urol. 1999 Jan;161(1):290-7 PMID: 10037426
  32. Microgravity tissue engineering.
    In Vitro Cell Dev Biol Anim. 1997 May;33(5):381-5 PMID: 9196897
  33. Cell culture for three-dimensional modeling in rotating-wall vessels: an application of simulated microgravity.
    J Tissue Cult Methods. 1992;14(2):51-7 PMID: 11541102
  34. Hemopoiesis in bone marrow of monkeys after spaceflight.
    J Gravit Physiol. 2000 Jan;7(1):S129-34 PMID: 11543441
  35. The sympathetic nervous system and the physiologic consequences of spaceflight: a hypothesis.
    Am J Med Sci. 1994 Aug;308(2):126-32 PMID: 8042655
  36. Morphologic differentiation of colon carcinoma cell lines HT-29 and HT-29KM in rotating-wall vessels.
    In Vitro Cell Dev Biol. 1992 Jan;28A(1):47-60 PMID: 1730571
  37. The effect of space and parabolic flight on macrophage hematopoiesis and function.
    Exp Cell Res. 1995 Jan;216(1):160-8 PMID: 7813616
  38. Multiparameter analysis of transplantable hemopoietic stem cells: I. The separation and enrichment of stem cells homing to marrow and spleen on the basis of rhodamine-123 fluorescence.
    Exp Hematol. 1985 Nov;13(10):999-1006 PMID: 2865163
  39. Physiology in microgravity.
    J Appl Physiol (1985). 2000 Jul;89(1):379-84 PMID: 10904075
  40. Rotating-wall vessel coculture of small intestine as a prelude to tissue modeling: aspects of simulated microgravity.
    Proc Soc Exp Biol Med. 1993 Feb;202(2):181-92 PMID: 8424108
  41. Flow effects on prostacyclin production by cultured human endothelial cells.
    Science. 1985 Mar 22;227(4693):1477-9 PMID: 3883488
  42. Analysis and separation of murine bone marrow stem cells by H33342 fluorescence-activated cell sorting.
    Exp Hematol. 1983 Sep;11(8):701-8 PMID: 6195006
  43. Effects of spaceflight on rat erythroid parameters.
    J Appl Physiol (1985). 1996 Jul;81(1):117-22 PMID: 8828653
  44. Simulated microgravity conditions enhance differentiation of cultured PC12 cells towards the neuroendocrine phenotype.
    In Vitro Cell Dev Biol Anim. 1998 Apr;34(4):316-25 PMID: 9590505
  45. Decreased production of red blood cells in human subjects exposed to microgravity.
    J Lab Clin Med. 1995 Apr;125(4):442-9 PMID: 7706898
  46. Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage.
    J Orthop Res. 1999 Jan;17(1):130-8 PMID: 10073657
  47. Potential and distribution of transplanted hematopoietic stem cells in a nonablated mouse model.
    Blood. 1997 Jun 1;89(11):4013-20 PMID: 9166840
  48. Utilization of microgravity bioreactors for differentiation of mammalian skeletal tissue.
    J Cell Biochem. 1993 Mar;51(3):252-6 PMID: 8501126
  49. Compartmentation of deoxypyrimidine nucleotides for nuclear DNA replication in S phase mammalian cells.
    J Mol Recognit. 1989 Sep;2(2):75-83 PMID: 2636898
  50. The capacity of connective tissue stromas to sustain myelopoiesis depends both upon the growth factors and the local intercellular environment.
    Biol Cell. 2000 Dec;92(8-9):605-14 PMID: 11374439
  51. The effect of fluid mechanical stress on cellular arachidonic acid metabolism.
    Ann N Y Acad Sci. 1987;516:513-24 PMID: 3439746
  52. Establishment of a three-dimensional human prostate organoid coculture under microgravity-simulated conditions: evaluation of androgen-induced growth and PSA expression.
    In Vitro Cell Dev Biol Anim. 1997 May;33(5):375-80 PMID: 9196896
  53. Induction of three-dimensional assembly of human liver cells by simulated microgravity.
    In Vitro Cell Dev Biol Anim. 1999 Oct;35(9):501-9 PMID: 10548431
  54. The fluctuating phenotype of the lymphohematopoietic stem cell with cell cycle transit.
    J Exp Med. 1998 Jul 20;188(2):393-8 PMID: 9670051
  55. Shear stress effects on human embryonic kidney cells in Vitro.
    Biotechnol Bioeng. 1985 Jul;27(7):1021-6 PMID: 18553772
  56. Adhesion-guided in vitro morphogenesis in pure and mixed cell cultures.
    Microsc Res Tech. 1998 Dec 1;43(5):379-84 PMID: 9858335
  57. Proliferation of human hematopoietic bone marrow cells in simulated microgravity.
    In Vitro Cell Dev Biol Anim. 2001 Feb;37(2):73-8 PMID: 11332740
  58. The anemia of microgravity and recumbency: role of sympathetic neural control of erythropoietin production.
    Acta Astronaut. 1994 Jul;33:137-41 PMID: 11539513
Article Info
Journal
In vitro cellular & developmental biology. Animal
Abbr.
In Vitro Cell Dev Biol Anim
ISSN
1071-2690
DOI
10.1290/1071-2690(2002)038<0343:ROEAAC>2.0.CO;2
Published
2002-06-00
Pages
343-51
Language
English
Region
Germany
NLM ID
9418515
Subset
IM
External Links
PubMed source DOI: 10.1290/1071-2690(2002)038<0343:ROEAAC>2.0.CO;2 View journal details
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